O objetivo desta pesquisa, de delineamento quase-experimental, foi verificar os efeitos da intervenção motora em três diferentes contextos - abordagens individual no domicílio (ID), individual na creche (IC) e grupo na creche (GC) - no desenvolvimento motor e social de crianças com atrasos motores, na faixa etária entre 06 e 18 meses de idade, residentes em bairros da periferia do município de Porto Alegre/RS, assistidas por unidades básicas de saúde vinculadas ao Hospital Moinhos de Vento e amparadas por creches comunitárias conveniadas com a prefeitura. Descrições do comportamento de cada criança antes e após o período interventivo e características do seu ambiente domiciliar foram associadas ao seu desempenho motor. A amostra desta pesquisa foi voluntária, selecionada de forma não-probabilística e intencional. A partir de uma população de 96 crianças avaliadas, 40 apresentaram percentil de desempenho motor abaixo de 25%, e destas, 32 crianças finalizaram as 24 sessões de intervenção motora, no período de 12 semanas. Cada grupo interventivo ficou assim distribuído: ID n=12, IC n=7 e GC n=13. Os instrumentos utilizados neste estudo foram a escala comportamental das Bayley Scales of Infant Development II (BSID-II), a Alberta Infant Motor Scale (AIMS) e o questionário Affordances in the Home Environment for Motor Development Self-Report (AHEMD-SR). O programa de intervenção, realizado entre maio e novembro de 2007, foi constituído por atividades lúdicas com o intuito de alongar e adequar o tônus muscular, estimular o acompanhamento visual e auditivo, controlar as posturas em diferentes circunstâncias, manipular objetos e deslocar-se, a fim de oportunizar às crianças a experimentação de novas condições de movimento e a superação de desafios. Todos os dados coletados foram analisados através do programa estatístico SPSS para Windows, versão 13.0. Para estas análises, o nível de significância adotado foi p≤0,05. O resultado do Teste de Normalidade Shapiro-Wilk, aplicado nos escores de desempenho motor na primeira avaliação, sugere que os dados não se apresentaram normalmente distribuídos (p=0,04). Desta forma, foram utilizados testes não-paramétricos. Na comparação das variáveis nos dois períodos avaliativos (pré e pós intervenção), o Teste de Kruskal-Wallis foi utilizado e, na presença de diferença significativa entre os grupos, o Teste Complementar de Mann-Whitney. Na comparação do percentil de desempenho motor de cada grupo ao longo do tempo (antes e após o período interventivo), com a finalidade de verificar o efeito do programa de intervenção motora, aplicou-se o Teste de Wilcoxon. Na descrição dos resultados, utilizou-se a mediana e a amplitude interquartílica (P25-P75). Os resultados evidenciaram mudanças significativas no desempenho motor nos três diferentes grupos (ID p=0,014, IC p=0,028 e GC p=0,005), e suportam a hipótese de que crianças com atrasos motores participantes do programa de intervenção apresentariam incremento no seu desempenho motor quando comparadas à avaliação inicial. As observações do comportamento das crianças desta amostra corroboram o pressuposto de que elas apresentariam, após a intervenção, incremento no seu desempenho social. A análise da variação dos escores totais de oportunidades de estimulação no lar, utilizando-se o Teste Qui-quadrado de McNemar, não evidenciou mudanças estatisticamente significativas nos diferentes grupos do estudo (p=1,000), rejeitando o pressuposto de que o programa de intervenção teria impacto positivo no contexto familiar, favorecendo mudanças significativas de engajamento nas diferentes atividades infantis rotineiras, bem como nas atitudes familiares frente às restrições destas crianças. Estratégias interventivas vinculadas ao contexto do desenvolvimento de crianças com atrasos motores promovem incremento em seu desempenho motor e social, e a identificação da criança de risco permite o acompanhamento precoce e a compreensão de suas necessidades. Estes achados sugerem a necessidade de programas de intervenção para famílias de baixa renda que otimizem as oportunidades de estimulação também no ambiente domiciliar. / The purpose of the present study, of quasi-experimental design, was to verify the effects of a motor intervention in three different contexts - individual at home (ID), individual at the daycare (IC) and in groups at the daycare (GC) - on motor and social development of children with motor delays, between 06 and 18 month-old, living in the suburbs of the city of Porto Alegre/RS, who were assisted by basic units of health affiliated to Moinhos de Vento Hospital and supported by communitarian daycare centers linked to the City Hall. Descriptions of the behavior of each child before and after the interventive period and characteristics of their home environment were associated to their motor performance. The sample for this research was voluntary, selected in a non probabilistic and intentional form. From a total of 96 assessed children, 40 presented percentile ranking below 25%, and, of those, 32 finished the 24 sessions of motor intervention, in a period of 12 weeks. Each interventive group was thus distributed: ID n=12, IC n=7 and GC n=13. The instruments used in this study were the behavior rating scale of Bayley Scales of Infant Development II (BSID-II), the Alberta Infant Motor Scale (AIMS) and the inventory Affordances in the Home Environment for Motor Development Self-Report (AHEMD-SR). The intervention program, carried out between May and November of 2007, was composed by playful activities which were planned to stretch and adjust the muscular tonus, to stimulate visual and auditory accompaniment, to control postures in different circumstances, to manipulate objects and to move around, in order to allow the children to experiment with new movement conditions and the overcoming of challenges. All the collected data were analyzed through the SPSS statistical program for Windows, version 13.0. In all the analyses, it was considered a significance level of p≤0,05. The result of the Shapiro-Wilk Normality Test, applied in the scores of motor performance in the first evaluation, suggested that the data was a non-normal distribution (p=0,04). Thus, non-parametric tests were used. In the comparison of the variables in the two evaluative periods (pre and post intervention), the Kruskal-Wallis’ Test was used and, when this test demonstrated significant differences between groups, the Mann-Whitney’s U Test was used. In the comparison of the percentile of motor performance of each group throughout time (before and after the interventive period), intending to verify the effect of the motor intervention program, the Wilcoxon Test was applied. In the description of the results, the median and the interquartilic range were used (P25-P75). The results showed significant improvement in the motor performance in the three different groups (ID p=0,014, IC p=0,028 and GC p=0,005), supporting the conviction that children with motor delays taking part in the intervention program would present increase in their motor performance when compared to the initial evaluation. The observations of children’s behavior from this sample corroborate with the conviction that they would show, after the intervention, increase in their social performance. The analysis of the variation of the total scores for opportunities of home stimulation, using McNemar Test, did not show evidence of significant statistical changes in the different study groups (p=1,000), rejecting the conviction that the intervention program would have a positive impact in the home environment, favoring significant changes of engagement in different infant routine activities, as well as in a shift in family attitude towards these children’s restrictions. Interventive strategies tied to the context of development of children with motor delays promote increase in their motor and social performance, and the identification of a risk child allows early accompaniment and the understanding of their necessities. These findings suggest the need of programs of intervention for low income families who also optimize the chances of stimulation in the home environment.

In the thesis on the pro-social traits of workers in helping (assistance-providing) professions I dedicate myself to pro-social behavior, empathy and the basic characteristics of personality issuing from the five-factor personality model {--} The Big Five. Most professionals agree that pro-social behavior and empathy belong among the basic characteristics that assistance-providing professionals should have. An important role is also the personality of the care provider. The listed constructs and connections among them however remain the home environment of empirical research and thereby represent the phenomena regarding which there exist few verified findings. The goal of the paper rests in fining the level of empathy and personal dispositions in accordance with the Big Five model that could influence pro-social behavior in selected groups of assistance-giving professionals. The factors in question are neuroticism, extroversion, and openness to experience, graciousness and conscientiousness. Data collection took place with the help of two psychodiagnostic questionnaires. The IRI questionnaire measures level of empathy and the NEO-FFI questionnaire was used for diagnosing the five personality dimensions. The study was quantitative. The monitored set included respondents from three assistance-giving professions {--} nurses, members of the Czech Republic Fire Rescue Corps, and elementary-school teachers. The results confirmed the hypothesis that care providing workers show higher levels of empathy in comparison to the population average, and the hypothesis that these professionals show a higher level of personality traits that, in an interpersonal context, display positive orientation towards others, that is that they are more extroverted and gracious in comparison with the population average, was refuted. These results make it possible to consider that the relationship between the basic personality dimensions and pro-social tendencies may be mediated by the level of empathy with regard to other psychological constructs and the results of social learning. Conclusions cannot be generalized, due to a relatively small sample group. For this purpose it would be interesting to carry out a professional study, which would exceed, in its scope and possibilities, the usual requirements of a thesis paper.

O concentrado de granulócitos é um importante componente para o tratamento de pacientes oncológicos. A doação deste componente é mais complexa do que outras doações de sangue ou hemocomponentes, porque necessita de maiores esforços e assumção de riscos por parte do doador e, também, o uso de equipamento automatizado de aférese. O presente estudo tem o objetivo de aumentar o conhecimento sobre a população de doadores de granulócitos e avaliar a diferença entre suas motivações para realizarem as doações e seu capital social geral comparando com doadores de plaquetas e doadores altruistas de sangue total. Com esses resultados os hemocentros que realizam a coleta de granulócitos não só poderão ter informações mais objetivas que tornem mais eficaz o recrutamento deste tipo de doador e, consequentemente, aumentar a disponibilidade deste componente como também, identificando um perfil semelhante dos doadores de granulócitos na população geral de doadores o banco de sangue poderá fidelizar estes indivíduos e promover doações de sangue mais seguras. O perfil dos doadores de granulócitos foi avaliado a partir de um estudo caso-controle, sendo o grupo casos formado por estes doadores (n=64) e, os controles por doadores de plaquetas (n-64) e de sangue total (n=68), na razão de um caso para dois controles. Doadores de granulócitos são principalmente do sexo masculino e com idade mais avançada do que os controles. Com referência às motivações para doarem, doadores de granulócitos não se diferem substancialmente dos doadores de plaquetas, mas em relação aos doadores de primeira vez de sangue total há diferenças consideráveis principalmente no que diz respeito ao interesse nos resultados dos testes sorológicos. Doadores de sangue total são quase duas vezes mais motivados a doarem pelo recebimento dos resultados comparados aos doadores de granulócitos, bem como também se motivam mais a doarem para tirar o dia de folga do trabalho o que indica um gesto de menor altruísmo destes daodores. O perfil sócio determinante entre os grupos de doadores também se difere, doadores de granulócitos são pessoas efetivamente mais engajadas socialmente comparados aos controles e têm de uma rede comunitária mais coesa e confiável, concordando cerca de duas vezes mais do que doadores de plaquetas que têm vizinhos conhecidos que os ajudaria financeiramente (OR=2,49) ou concordando quatro vezes mais que doadores de sangue total que têm vizinhos conhecidos que estariam sempre prontos a ajudar outros vizinhos (OR=4,02). Concluimos que os resultados indicam que há a necessidade dos bancos de sangue utilizarem novas estratégias de recrutamento para aumentar a conversão de doadores, passando a utilizar, além do recrutamento interno, comunicações mais efetivas que, consigam atingir a população foco com maior eficiência, em locais onde a probabilidade de encontrar doadores mais engajados socialmente é aumentada, como clubes, centros comunitários ou associações / The concentrate of granulocytes is an important component for oncologic patient\'s treatment. Donation of this component is more complex than other blood components donations, because it claims more efforts and risks assumptions by the donor, and also the use of automated apheresis equipment. The present study is aimed to increase knowledge about the granulocyte donors´ population and evaluate differences among their motivations to accomplish donations and their general social capital, comparing with platelets apheresis donors and altruistic whole blood donors. These results may help blood centers, which carry granulocyte collection, not just to achieve much more objective information about these donors, but also to identify similar granulocyte donors´ profile within the general population. Blood banks may be able to achieve fidelity among these individuals and to promote safer blood donations. Granulocyte donors´ profile was evaluated in a case-control study. Cases were formed by granulocytes donors (n=64) and controls by platelet apheresis donors (n-64) and first time whole blood donors (n=68), in a ratio of one case per two controls. Granulocyte donors are mainly male and older than controls. Regarding the motivation to donate, granulocyte donors are not essentially different from platelets donors, but regarding first time whole blood donors, there are considerable differences, especially in respect to test seeking. Moreover, whole blood donors are almost twice more motivated to donate to take a day off at work, which shows a minor altruistic gesture. The determinant social profile among donors´ groups is also different. Granulocyte donors are effectively more social engaged people when compared to controls, and have a much more coherent and trustful community net, assenting about twice more than platelets donors, which have acquainted neighbors that would be ever ready to help other neighbors (OR=4.02). In conclusion, these findings indicate that is necessary to blood centers to use new recruitment strategies to increase donor´s retention. More effective communications procedures, besides internal recruitment, that achieves the target with more efficiency in locals where the probability to find donors more social engaged is increased, as clubs, community centers or associations must be warranted

O objetivo desta pesquisa, de delineamento quase-experimental, foi verificar os efeitos da intervenção motora em três diferentes contextos - abordagens individual no domicílio (ID), individual na creche (IC) e grupo na creche (GC) - no desenvolvimento motor e social de crianças com atrasos motores, na faixa etária entre 06 e 18 meses de idade, residentes em bairros da periferia do município de Porto Alegre/RS, assistidas por unidades básicas de saúde vinculadas ao Hospital Moinhos de Vento e amparadas por creches comunitárias conveniadas com a prefeitura. Descrições do comportamento de cada criança antes e após o período interventivo e características do seu ambiente domiciliar foram associadas ao seu desempenho motor. A amostra desta pesquisa foi voluntária, selecionada de forma não-probabilística e intencional. A partir de uma população de 96 crianças avaliadas, 40 apresentaram percentil de desempenho motor abaixo de 25%, e destas, 32 crianças finalizaram as 24 sessões de intervenção motora, no período de 12 semanas. Cada grupo interventivo ficou assim distribuído: ID n=12, IC n=7 e GC n=13. Os instrumentos utilizados neste estudo foram a escala comportamental das Bayley Scales of Infant Development II (BSID-II), a Alberta Infant Motor Scale (AIMS) e o questionário Affordances in the Home Environment for Motor Development Self-Report (AHEMD-SR). O programa de intervenção, realizado entre maio e novembro de 2007, foi constituído por atividades lúdicas com o intuito de alongar e adequar o tônus muscular, estimular o acompanhamento visual e auditivo, controlar as posturas em diferentes circunstâncias, manipular objetos e deslocar-se, a fim de oportunizar às crianças a experimentação de novas condições de movimento e a superação de desafios. Todos os dados coletados foram analisados através do programa estatístico SPSS para Windows, versão 13.0. Para estas análises, o nível de significância adotado foi p≤0,05. O resultado do Teste de Normalidade Shapiro-Wilk, aplicado nos escores de desempenho motor na primeira avaliação, sugere que os dados não se apresentaram normalmente distribuídos (p=0,04). Desta forma, foram utilizados testes não-paramétricos. Na comparação das variáveis nos dois períodos avaliativos (pré e pós intervenção), o Teste de Kruskal-Wallis foi utilizado e, na presença de diferença significativa entre os grupos, o Teste Complementar de Mann-Whitney. Na comparação do percentil de desempenho motor de cada grupo ao longo do tempo (antes e após o período interventivo), com a finalidade de verificar o efeito do programa de intervenção motora, aplicou-se o Teste de Wilcoxon. Na descrição dos resultados, utilizou-se a mediana e a amplitude interquartílica (P25-P75). Os resultados evidenciaram mudanças significativas no desempenho motor nos três diferentes grupos (ID p=0,014, IC p=0,028 e GC p=0,005), e suportam a hipótese de que crianças com atrasos motores participantes do programa de intervenção apresentariam incremento no seu desempenho motor quando comparadas à avaliação inicial. As observações do comportamento das crianças desta amostra corroboram o pressuposto de que elas apresentariam, após a intervenção, incremento no seu desempenho social. A análise da variação dos escores totais de oportunidades de estimulação no lar, utilizando-se o Teste Qui-quadrado de McNemar, não evidenciou mudanças estatisticamente significativas nos diferentes grupos do estudo (p=1,000), rejeitando o pressuposto de que o programa de intervenção teria impacto positivo no contexto familiar, favorecendo mudanças significativas de engajamento nas diferentes atividades infantis rotineiras, bem como nas atitudes familiares frente às restrições destas crianças. Estratégias interventivas vinculadas ao contexto do desenvolvimento de crianças com atrasos motores promovem incremento em seu desempenho motor e social, e a identificação da criança de risco permite o acompanhamento precoce e a compreensão de suas necessidades. Estes achados sugerem a necessidade de programas de intervenção para famílias de baixa renda que otimizem as oportunidades de estimulação também no ambiente domiciliar. / The purpose of the present study, of quasi-experimental design, was to verify the effects of a motor intervention in three different contexts - individual at home (ID), individual at the daycare (IC) and in groups at the daycare (GC) - on motor and social development of children with motor delays, between 06 and 18 month-old, living in the suburbs of the city of Porto Alegre/RS, who were assisted by basic units of health affiliated to Moinhos de Vento Hospital and supported by communitarian daycare centers linked to the City Hall. Descriptions of the behavior of each child before and after the interventive period and characteristics of their home environment were associated to their motor performance. The sample for this research was voluntary, selected in a non probabilistic and intentional form. From a total of 96 assessed children, 40 presented percentile ranking below 25%, and, of those, 32 finished the 24 sessions of motor intervention, in a period of 12 weeks. Each interventive group was thus distributed: ID n=12, IC n=7 and GC n=13. The instruments used in this study were the behavior rating scale of Bayley Scales of Infant Development II (BSID-II), the Alberta Infant Motor Scale (AIMS) and the inventory Affordances in the Home Environment for Motor Development Self-Report (AHEMD-SR). The intervention program, carried out between May and November of 2007, was composed by playful activities which were planned to stretch and adjust the muscular tonus, to stimulate visual and auditory accompaniment, to control postures in different circumstances, to manipulate objects and to move around, in order to allow the children to experiment with new movement conditions and the overcoming of challenges. All the collected data were analyzed through the SPSS statistical program for Windows, version 13.0. In all the analyses, it was considered a significance level of p≤0,05. The result of the Shapiro-Wilk Normality Test, applied in the scores of motor performance in the first evaluation, suggested that the data was a non-normal distribution (p=0,04). Thus, non-parametric tests were used. In the comparison of the variables in the two evaluative periods (pre and post intervention), the Kruskal-Wallis’ Test was used and, when this test demonstrated significant differences between groups, the Mann-Whitney’s U Test was used. In the comparison of the percentile of motor performance of each group throughout time (before and after the interventive period), intending to verify the effect of the motor intervention program, the Wilcoxon Test was applied. In the description of the results, the median and the interquartilic range were used (P25-P75). The results showed significant improvement in the motor performance in the three different groups (ID p=0,014, IC p=0,028 and GC p=0,005), supporting the conviction that children with motor delays taking part in the intervention program would present increase in their motor performance when compared to the initial evaluation. The observations of children’s behavior from this sample corroborate with the conviction that they would show, after the intervention, increase in their social performance. The analysis of the variation of the total scores for opportunities of home stimulation, using McNemar Test, did not show evidence of significant statistical changes in the different study groups (p=1,000), rejecting the conviction that the intervention program would have a positive impact in the home environment, favoring significant changes of engagement in different infant routine activities, as well as in a shift in family attitude towards these children’s restrictions. Interventive strategies tied to the context of development of children with motor delays promote increase in their motor and social performance, and the identification of a risk child allows early accompaniment and the understanding of their necessities. These findings suggest the need of programs of intervention for low income families who also optimize the chances of stimulation in the home environment.

INTRODUÇÃO: A vivência de maus tratos na infância é apontada como um fator de risco para o desenvolvimento de comportamentos antissociais e traços psicopáticos. Estudos sugerem alterações estruturais de substância branca (SB) cerebral em vítimas de maus tratos, que podem estar subjacentes a sintomas psiquiátricos e dificuldades cognitivas. OBJETIVO: Investigar o comportamento social de adolescentes vítimas de maus tratos (A-VMT), através da comparação de suas habilidades sociais e traços de psicopatia com um grupo controle (GC), e de possíveis correlações destas medidas com os diferentes tipos de maus tratos e com o volume de SB cerebral. MÉTODO: Foram avaliados 67 A-VMT e 41 adolescentes do GC através do Questionário de Traumas na Infância (QUESI), da Psychopathy Checklist Youth Version (PCL-YV) e do Inventário de Habilidades Sociais para Adolescentes (IHSA). Também foram adquiridas imagens anatômicas cerebrais através de equipamento de ressonância magnética (1,5T Siemens Sonata) e realizadas análises de morfometria baseada em voxels (VBM). RESULTADOS: Os A-VMT apresentaram maior intensidade de traços psicopáticos e mais déficits de habilidades sociais que o GC. A negligência emocional foi a forma de maus tratos que mais influenciou a presença de traços de psicopatia e que mais associou-se a prejuízos de habilidades sociais específicas. O volume de SB de regiões do hemisfério esquerdo (giro angular, precuneus e lobo parietal inferior) correlacionouse negativamente com o fator afetivo da PCL:YV. Além disso, foram encontradas correlações positivas entre o volume de SB de regiões de hemisfério direito com fatores de psicopatia: o volume do lobo parietal superior direito correlacionou-se com características interpessoais e o do giro pré-central com o fator antissocial. CONCLUSÃO: Os A-VMT apresentaram déficits do comportamento social quando comparados ao GC. O volume de SB de áreas cerebrais envolvidas no processamento de informações sociais e reconhecimento de emoções se correlacionou com traços específicos da psicopatia. A vivencia de maus tratos na infância pode contribuir para déficits na cognição social, o que por sua vez, pode predispor esta vulnerável população a alguns comportamentos antissociais / Introduction: Child maltreatment is considered a risk factor for the development of antisocial behaviors and psychopathic traits. Studies suggest that specific white matter tracts may be vulnerable to child maltreatment and their alterations can be associated with psychiatric symptoms and cognitive deficits. Aim: Compare social skills and psychopathic traits between maltreated adolescents (MTA) and a control group, as well as their possible correlations with different types of maltreatment and white matter volumes.. Method: The sample was composed by 67 MTA and 41 youths from the CG. Brain images were acquired by magnetic resonance imaging equipment (1,5T Siemens Sonata) for voxel-based morphometry analyses. The clinical evaluation was carried out using Childhood Trauma Inventory (CTQ), Social Skills Inventory for Adolescents (SSIA) and The Hare Psychopathy Checklist: Youth Version (PCL:YV). Results: MTA presented more psychopathic traits and social skills deficits than the CG. Emotional neglect was the only maltreatment subtype with significant effect on psychopathic traits, and it was correlated with specific social skills deficits. The white matter volumes of left hemisphere regions (angular gyrus, precuneus and inferior parietal lobe) were negatively correlated with PCL:YV affective factor scores. Moreover, positive correlations between white matter volume of right hemisphere areas (superior parietal lobe and precentral gyrus) and specific psychopathic traits (antisocial and interpersonal) were found. Conclusion: MTA presented social deficits when compared to CG. The white matter volume of brain areas associated with social information processing and emotion recognition was correlated with specific psychopathic traits. Child maltreatment may contribute to social cognition deficits and predispose this vulnerable population to psychopathic traits

Cette thèse d’anthropologie a pour ambition d’explorer un objet peu étudié par la discipline. Elle porte un regard critique sur les déchets recyclables dans un contexte spécifique, celui d’un dispositif de tri sélectif en France, à Marseille. Moins étudié que d’autres catégories de déchets à l’instar des déchets organiques, industriels ou énergétiques… cette thèse s’intéressera plus particulièrement aux dimensions sociales et culturelles du déchet recyclable en le reliant et le comparant à celui de l’ensemble des autres déchets domestiques (déchets organiques, toxiques, encombrants…). L’objectif étant de révéler les représentations et pratiques rattachées à ces différents types de déchets, les jeux et logiques des acteurs face au dispositif de collecte en place ainsi que les trajectoires et les mises à distance spatio-temporelles suivies par ces déchets en fonction de leur catégorie. / This thesis Anthropology aims to explore a little-studied subject matter in this field. It takes a critical look at the recyclable waste in a specific context: the sorting device system in Marseille, France. Less studied than other waste categories like organic waste, industrial or energy ... this thesis will focus on the social and cultural dimensions of recyclable waste by connecting and comparing it with all other domestic waste (organic waste, toxic, cumbersome ...). The aim is to reveal the representations and practices associated with these different types of waste; the games and actors’ logic facing the collection device in place; the paths and spatiotemporal storing areas followed by the waste according to their category.

La vie en société offre de nombreux avantages, mais comporte toutefois certains risques comme celui de la prolifération des pathogènes. Cette susceptibilité accrue s'explique notamment par la densité élevée des individus au sein des colonies, la proximité génétique et la fréquence des interactions sociales. Au sein du nid, les cadavres peuvent être porteurs de pathogènes :ils représentent un risque important pour la survie de la colonie entière et doivent donc en être écartés en permanence. L’objectif général de cette thèse est d’améliorer la compréhension des mécanismes individuels et collectifs qui régissent le rejet de ces cadavres à l’extérieur de la colonie chez la fourmi rouge, Myrmica rubra. <p><p>Tout d’abord, nous avons mis en évidence l’importance du rejet des déchets pour la survie des fourmis (Chapitre 1). Le rejet des cadavres, même exempts de pathogènes, permet d’augmenter légèrement la survie des ouvrières. La nécrophorèse prend toute son importance lorsque la colonie est exposée à des cadavres infectés par le champignon Metarrhizium anisopliae. Une limitation du rejet de ces cadavres porteurs de pathogènes entraîne une mortalité des ouvrières relativement importante (jusqu’à 30% après 50 jours) tandis que celle des larves reste très limitée. Nous avons également cherché à identifier les composés chimiques susceptibles de déclencher le rejet des cadavres par leurs congénères (Chapitre 2). Les cadavres fraîchement tués n’étaient pas rejetés rapidement. Par contre, des cadavres vieux de 1 à 6 jours étaient presque toujours éloignés du nid. Sur ces corps « âgés » de 1 à 6 jours, on constate l’apparition de deux composés :les acides oléique et linoléique. L’adjonction sur des cadavres frais d’acide oléique et/ou d’acide linoléique en quantités équivalentes à celles trouvées sur un cadavre de plus de 24h en provoque le rejet par les ouvrières. Nous avons ensuite étudié les facteurs susceptibles d’influencer le lieu de dépôt des cadavres à l’extérieur du nid (Chapitre 3). Les cadavres ne sont pas entassés dans un endroit particulier, mais dispersés autour du nid, relativement loin de l’entrée de celui-ci. De plus, le marquage passif des zones explorées par les ouvrières -qui sont un indice de fréquentation de cette zone par la colonie- n’influence pas la décision des ouvrières d’y déposer le cadavre. Lors du transport des corps, nous avons étudié quels sont les moyens utilisés par les fourmis pour leur orientation (Chapitre 4). Aucune clé chimique n’intervient dans l’orientation des fourmis transporteuses mais celles-ci utilisent leur mémoire spatiale en retournant préférentiellement dans la direction déjà visitée. Enfin, nous avons testé s’il existe une spécialisation à court ou moyen terme des ouvrières dans le transport de cadavres (Chapitre 5). Nous avons pu montrer que les fourmis transporteuses de corps peuvent se spécialiser lors de transports successifs à court terme (de l’ordre d’une heure). Par contre, aucune spécialisation dans les activités de nécrophorèse n’a pu être mise en évidence à moyen terme (de l’ordre de quelques semaines). Les fourmis transporteuses de cadavres sont le plus souvent des ouvrières actives à l’extérieur du nid qui n’ont que peu de contacts avec leurs congénères au sein du nid ou avec les stades particulièrement sensibles aux pathogènes tels que les larves.<p><p>L’ensemble des comportements liés au rejet et au transport des cadavres s’inscrivent dans les stratégies prophylactiques et hygiéniques de la colonie. Nous discuterons des liens entre le rejet des cadavres et l’ensemble des comportements appartenant à l’immunité sociale, qui permettent de limiter la prévalence et la propagation des pathogènes chez les insectes sociaux.<p> / Doctorat en Sciences agronomiques et ingénierie biologique / info:eu-repo/semantics/nonPublished

Extensive serotonin (5-HT) fiber innervation throughout the brain corroborates 5-HT’s modulatory role in numerous behaviors including social behavior, emotion regulation, and learning and memory. Abnormal brain 5-HT levels and function are implicated in Autism Spectrum Disorder (ASD) which often co-occurs with other neuropsychiatric conditions. While 5-HT therapeutics are used to treat ASD, variable improvements in symptomatology require further investigation of 5-HT-mediated pathology. Neurexins (Nrxns) are presynaptic cell adhesion molecules that maintain synapse function for proper neural circuit assembly. Given that aberrant Nrxn and 5-HT function independently contribute to signaling pathology and behavioral impairments, it is critical to understand how Nrxn-mediated 5-HT neurotransmission participates in pathological mechanisms underlying ASD. Using fluorescence in situ hybridization, I found that the three Nrxn genes (Nrxn1, Nrxn2, and Nrxn3) are differentially expressed in 5-HT neurons in the dorsal raphe nucleus (DRN) and median raphe nucleus which contain the primary source of 5-HT neurons in the brain. Our lab generated a mouse model with selective deletion of Nrxns in 5-HT neurons to investigate the function of Nrxns in 5-HT signaling. The loss of Nrxns at 5-HT release sites reduced 5-HT release in the DRN and hippocampus and altered 5-HT innervation in specific brain regions. The lack of 5-HTergic Nrxns also reduced sociability and increased depressive-like behavior in males. This mouse model provides mechanisms to shed new light on 5-HT neurotransmission in the generation of complex behaviors.

Die wissenschaftliche Erforschung des Haushundes (Canis lupus forma familiaris) mit Fokus auf seine sozialen Eigenschaften hat große Relevanz in Anbetracht seiner zunehmenden Bedeutung im Zusammenleben mit dem Menschen als Familienmitglied und Arbeitstier. Ziel der vorliegenden Studie war es, die Relevanz von Dominanz in den Beziehungen von Gruppenmitgliedern einer Meute Englischer Foxhounds darzustellen sowie ihre Auswirkung auf die Sozialstruktur der Hunde. Weitergehend sollte die Analyse von aggressionsanzeigendem Verhalten und sozialen Strategien Aufschluss über Regelungsmechanismen im Zusammenleben der Hunde geben. Speichelcortisolanalysen sollten Einblick über die Stressexposition der Hunde in diesem Zusammenleben geben. Das Neutrophilen/ Lymphozyten Verhältnis im Blut sollte darüber hinaus mögliche Auswirkungen der Cortisolproduktion auf das Immunsystem aufzeigen. Für die Datenerhebung wurden 16 Englische Foxhounds einer Meute von 72 Hunden berücksichtigt. Je 4 Hündinnen und 4 Rüden wurden einer von 2 Gruppen zugeteilt. Über einen Zeitraum von 10 Monaten erfolgten Verhaltensbeobachtungen an jedem Individuum mittels der Methode Focal Animal Sampling. Von Hunden aus Gruppe 1 wurden 17,5 Stunden Beobachtungsmaterial und aus Gruppe 2 17,0 Stunden gesammelt. Die Speichelproben wurden mit der Salivette® der Firma SARSTEDT (Nümbrecht- Rommelsdorf) entnommen und mittels eines speziell für die Speichelcortisol- Analyse entwickelten Immunoassays (double- antibody biotin- linked enzyme immunoassay) analysiert. Ergebnisse: Die als möglicherweise dominanzanzeigend eingestuften Indikatoren, nämlich die Körperhaltung High Posture und die Durchsetzungskraft als Eigenschaft eines Hundes, konnten als solche nicht bestätigt werden. Es konnte keine dominanzbasierte Hierarchie ermittelt werden. Auch konnten von 82 Dyaden nur in 5 eine Dominanz- Subordinanz- Beziehung ermittelt werden, was kein aussagekräftiges Ergebnis darstellt. Aggressive Handlungen wurden in beiden Gruppen pro Stunde durchschnittlich jeweils 12 Mal beobachtet, jedoch meist in der Intensität 1 - 2 (von 5- stufiger Skala). Sie wurden hochsignifikant häufiger unter Rüden als unter Hündinnen beobachtet (p= 0,002). Berechnungen der Aggressionskoeffizienten belegten deutliche Unterschiede in den individuellen Aggressionsniveaus. Die Analyse ausgetragener Konflikte auf Taktiken und Strategien lieferte jedoch keine signifikanten Ergebnisse. Die Foxhounds reagierten situativ und selten gegner- und nie kontextabhängig. Die Speichelcortisolanalysen belegten vielfach höhere Basal- Mittelwerte der Foxhounds verglichen mit Analysen von Hunden anderer Studien. Eine Reduktion des Cortisolwerts erfolgte bei 14 von 16 Hunden nach 20- minütigem Einzelspaziergang mit Menschenkontakt. Allerdings konnte keine Verbindung zwischen dem Speichelcortisolwert und dem soziometrisch errechneten Rangplatz in der Gruppe ermittelt werden. Ebenso bestand keine Korrelation zwischen dem Speichelcortisolwert und Immunstatus sowie Aggressionspotential. Schlussfolgerungen: Dominanz spielte keine erkennbare Rolle in den sozialen Interaktionen und Beziehungen der Hunde. Die Ergebnisse lassen die Schlussfolgerung auf eine egalitäre Sozialstruktur der Foxhounds zu statt einer dominanzabhängigen, was auch durch die situativ geregelten Begegnungen unterstützt wird. Aggressives Verhalten als häufig repräsentiertes Kommunikationselement wurde bei den einzelnen Hunden unterschiedlich oft beobachtet, was auf die Verschiedenheit der Charaktere und ihre Motivationen, Konflikte einzugehen, hindeutet. Die häufiger unter Rüden beobachtete Aggression kann auf ein erhöhtes Konkurrenzpotential hindeuten. Die im Speichel der Foxhounds gemessenen Cortisolwerte lassen die Vermutung auf eine durch das Gruppenleben bedingte Erhöhung des Basalwerts zu, ohne Konsequenzen auf den Immunstatus. Die fehlenden Korrelationen zu den individuellen Rangplätzen und Aggression unterstützt die Vermutung eines lockeren bzw. egalitären Sozialgefüges.

Un nombre croissant d’études indique que les interventions musicales (IMs) ont des effets bénéfiques sur les enfants et adolescents atteints d’un trouble du spectre de l’autisme (TSA). À ce jour, aucune revue systématique utilisant une approche méta-analytique n’a investigué l’efficacité des IMs sur trois des symptômes prédominants du TSA, soit le fonctionnement social, les comportements mal-adaptés et le langage. Dans ce mémoire, tous les 17 articles scientifiques comparant les IMs à des interventions non-musicales (INMs) ont été décrits systématiquement et évalués selon les lignes directrices de CONSORT. Onze études répondaient aux critères d’inclusion des présentes méta-analyses, chacune rapportant des mesures d’évaluations longitudinales ou d’évaluations pendant les sessions. Les résultats des méta-analyses ont démontré un bénéfice des IMs chez le TSA, particulièrement pour les mesures des comportements sociaux mal-adaptés. Une comparaison entre les IMs et les INMs indiquait un avantage pour les IMs comparément aux INMs en ce qui concerne les comportements sociaux, mais aucun avantage pour les comportements mal-adaptés non-sociaux et le langage. La revue systématique a révélé des lacunes méthodologiques des études évaluées, telles que des tailles d’échantillons restreintes, des durées et intensités d’interventions limitées, un manque d’information à propos des échantillons et des critères d’appariement, puis le biais d’attrition. La combinaison de cette revue systématique et des méta-analyses a permis une mise à jour de l’évaluation des preuves de l’efficacité des IMs pour les jeunes ayant un TSA, ainsi que de donner des recommandations aux chercheurs et cliniciens afin d’améliorer la pratique dans ce domaine. / There is considerable interest in using music interventions (MIs) to address core impairments present in children and adolescents with autism spectrum disorder (ASD). An increasing number of studies suggest that MIs have positive outcomes in this population, but no systematic review employing meta-analysis to date has investigated the efficacy of MIs across three of the predominant symptoms in ASD, specifically social functioning, maladaptive behaviors and language impairments. In this thesis, all available peer-reviewed studies (n = 17) comparing active MIs to non-music interventions (NMIs) were systematically summarized and evaluated using quality assessment based on the CONSORT statement. Eleven studies fulfilled inclusion criteria for meta-analysis, which differentiated between within-session and longitudinal outcomes. The quantitative analyses results supported the effectiveness of MI in ASD, and particularly for measures sensitive to social maladaptive behaviors. Comparing between MI and NMI, the results were generally suggestive of benefits of MI over NMI for social outcomes, but did not find benefits for non-social maladaptive behaviors or language outcomes. The systematic review revealed important methodological issues present in these studies, such as small sample sizes, restricted durations and intensities of interventions, missing sample information and matching criteria, and attrition bias. Together, the combined systematic review and meta-analyses provided an up-to-date evaluation of the evidence for MI’s benefits in ASD children and provide key recommendations for future clinical interventions and research about best practice in the domain.