[pt] Uma propriedade notável dos sistemas biológicos é a formação de estruturas espaciais. Estas podem surgir por auto-organização, como consequência das próprias interações entre os indivíduos. Para estudar estas estruturas e como elas emergem, têm sido muito úteis modelos simples para a dinâmica da densidade espacial de uma população, que levam em conta apenas certos processos elementares (como reprodução, competição e dispersão). Em particular, a equação de FKPP (Fisher-Kolmogorov- Petrovski-Piskunov), que inclui simplesmente o crescimento logístico mais a difusão normal, é um modelo clássico para a dinâmica de uma população de uma única espécie. Dentro do quadro minimalista da equação de FKPP e suas variantes, a competição à distância (ou, não local) é a principal responsável por produzir oscilações espaciais na densidade da população. Entretanto, a não localidade pode ocorrer também nos demais processos. Assim, um primeiro objetivo desta tese é investigar como as diferentes escalas espaciais presentes podem interferir entre si, afetando a formação de padrões. Para isso, consideramos uma generalização da equação de FKPP em que todos os termos são não locais, em um ambiente homogêneo com condições de contorno periódicas. Enquanto a competição é o principal processo por trás da formação de padrões, mostramos que os outros dois podem agir de forma construtiva ou destrutiva. Por exemplo, a difusão, que comumente homogeniza, pode favorecer a formação de padrões dependendo do formato e alcance das funções de influência de cada processo. Em um segundo estudo, motivado por resultados experimentais, procuramos entender como a variabilidade da difusividade pode impactar a organização espacial da população dentro e fora de um refúgio (região de alta qualidade imersa em um ambiente hostil). Para tanto, consideramos uma outra generalização da equação de FKPP, com não localidade apenas no processo de competição intra-espécie, e modificada para levar em conta a presença do refúgio. Além da dependência espacial da taxa de crescimento, que é a principal característica distintiva de um refúgio em um ambiente hostil, também consideramos o fato de que a mobilidade pode ser heterogênea no espaço ou depender da densidade populacional. Focamos em dois casos em que a difusividade responde à densidade de indivíduos, diminuindo ou aumentando com a densidade populacional. Para comparação, também abordamos a difusividade dependente do espaço, com valores diferentes dentro e fora do refúgio. Observamos que o limiar da formação de padrões, no espaço de parâmetros, é bastante robusto diante destas heterogeneidades. Por outro lado, a dependência com a densidade pode produzir uma realimentação que está ausente em meios homogêneos, e que afeta a forma dos padrões. Em todos os casos, os resultados foram obtidos mediante a integração numérica das equações integro-diferenciais e realizando considerações analíticas. / [en] A remarkable property of biological systems is the formation of spatial structures. These can arise by self-organization, as a consequence of the interactions between individuals. To study these structures and how they emerge, simple models for the dynamics of the spatial density of a population, which take into account only certain elementary processes (such as reproduction, competition and dispersion) have been very useful. In particular, the FKPP (Fisher-Kolmogorov-Petrovski-Piskunov) equation, which simply includes logistic growth plus normal diffusion, is a classic model for the dynamics of a population of a single species. Within the minimalist framework of the FKPP equation and its variants, distance (or, non-local) competition is primarily responsible for producing spatial oscillations in population density. However, non-locality can also be present in other processes. Then, a first objective of this thesis is to investigate how the different spatial scales which are present in each process can interfere between them, affecting the formation of patterns in a homogeneous environment with periodic boundary conditions. For this purpose, we consider a generalization of the FKPP equation in which all terms are nonlocal. While competition is the main process behind pattern formation, we show that the other two can act constructively or destructively. For example, diffusion, which commonly homogenizes, can favor the formation of patterns depending on the format and range of the influence functions of each process. In a second study, motivated by experimental results, we seek to understand how the variability of the diffusivity can impact the spatial organization of the population inside and outside a refuge (a high-quality region immersed in a hostile environment). Therefore, we consider another generalization of the FKPP equation, with non-locality only in the intra-species competition process, modified to take into account the presence of the refuge. In addition to the spatial dependence of the growth rate, which is the main distinguishing feature of a refuge in a hostile environment, we also consider the fact that mobility can be spatially heterogeneous or depend on population density. We focus on two cases in which diffusivity responds to the density of individuals, decreasing or increasing with population density. For comparison, we also address spacedependent diffusivity, with different values inside and outside the refuge. We observed that the threshold of pattern formation in parameter space is quite robust under the presence of these heterogeneities. On the other hand, density dependence can produce a feedback that is absent in homogeneous media, and that affects the shape of the patterns. In all cases, the results were obtained by numerical simulations of the integro-differential equations and by analytical considerations.

The object of the study was the professional identity of students in the psychological areas of the university. The subject of the study was the relationship between professional identity and personal qualities of students. The master's thesis consists of an introduction, two chapters, a conclusion, a list of references (110 sources) and an appendix, which includes forms of the methods used. The volume of the master's thesis is 88 pages, which contain 11 figures and 11 tables. The introduction reveals the relevance of the research problem, the development of the problem, sets the goal and objectives of the study, defines the object and subject of the study, formulates the main and additional hypotheses, indicates the methods and empirical base, as well as the stages of the study, scientific novelty, theoretical and practical significance of the work. The first chapter includes a review of foreign and domestic literature on the topic of theoretical foundations for the study of student professional identity. Sections devoted to the study of the concepts of identity, psychological well-being, reflexivity, self-organization, resilience are presented. The conclusions on the first chapter are the results of the study of theoretical material. The second chapter is devoted to the empirical part of the study. It presents a description of the organization and methods of the study and the results obtained by all the methods used: the Questionnaire of the professional identity of psychology students by U. S. Rodygina, the scale of psychological well-being by K. Riff, the questionnaire of reflexivity by A. V. Karpov, the questionnaire of self-organization of activity by N. Feather and M. Bond, methodology for studying the motivation of a professional career E. Shane, S. Muddy's hardiness test. The chapter also presents a correlation analysis of the results of the study. Chapter 2's conclusions include the main findings of the empirical study. In conclusion, the results of the theoretical and empirical parts of the work, as well as conclusions on them, are summarized, the practical significance of the study is substantiated, and possible prospects for further development of this issue are described. / Объектом исследования явилась профессиональная идентичность студентов психологических направлений университета. Предметом исследования стала взаимосвязь профессиональной идентичности и личностных качеств студентов. Магистерская диссертация состоит из введения, двух глав, заключения, списка литературы (110 источников) и приложения, включающего в себя бланки применявшихся методик. Объем магистерской диссертации 88 страниц, на которых размещены 11 рисунков и 11 таблиц. Во введении раскрывается актуальность проблемы исследования, разработанность проблематики, ставятся цель и задачи исследования, определяются объект и предмет исследования, формулируются основная и дополнительные гипотезы, указываются методы и эмпирическая база, а также этапы проведения исследования, научная новизна, теоретическая и практическая значимость работы. Первая глава включает в себя обзор иностранной и отечественной литературы по теме теоретических оснований исследования профессиональной идентичности студент. Представлены разделы, посвященные исследованию понятий идентичность, психологическое благополучие, рефлексивность, самоорганизация, жизнестойкость. Выводы по первой главе представляют собой итоги по изучению теоретического материала. Вторая глава посвящена эмпирической части исследования. В ней представлено описание организации и методов проведенного исследования и результатов, полученных по всем использованным методикам: Опроснику профессиональной идентичности студентов-психологов У. С. Родыгиной, шкале психологического благополучия К. Рифф, опроснику рефлексивности А. В. Карпова, опроснику самоорганизации деятельности Н. Физер и М. Бонд, методике изучения мотивации профессиональной карьеры Э. Шейна, тесту жизнестойкости С. Мадди. Также в главе представлен корреляционный анализ результатов исследования. Выводы по главе 2 включают в себя основные результаты эмпирического исследования. В заключении в обобщенном виде изложены результаты теоретической и эмпирической частей работы, а также выводы по ним, обоснована практическая значимость исследования и описаны возможные перспективы дальнейшей разработки данной проблематики.

<p>Optical beams that travel through a material without undergoing divergence are known as self-trapped beams. Self-trapping occurs when a beam induces a suitable index gradient in the medium that is capable of guiding the original beam. An incoherent light consists of femtosecond scale speckles, due to random phase fluctuations and were not thought to self-trap until recently. In 1997, Mitchell et al., showed that white light can self-trap, provided the medium cannot respond fast enough to form index gradients to these speckles individually. However, detailed studies have been hampered by a lack of suitable materials and strategies for enabling such a response. In 2006, our group showed that a photopolymer is suitable for incoherent self-trapping, since the index change is governed by an inherently slow rate of polymerization (of the order of milliseconds). This has enabled further studies of various phenomena with white light self-trapping.</p> <p>The studies here show (i) the first direct experimental evidence of interactions of two incoherent white light self-trapped beams, as well as fission, fusion and repulsion. Existence of dark self-trapping beams with incoherent white light was also shown, counter intuitively in a positive nonlinear medium. (iii) Lattices were formed with multiple ordered bright as well as dark self-trapping filaments using optochemical self-organization. (iv) Woodpile-like 3D lattices with bright and dark beams were also demonstrated and simulations showed theoretical band gaps. (v) Self-trapping of a co-axial beam of incoherent white light was also shown experimentally and through simulations.</p> / Doctor of Philosophy (PhD)

Distributed systems are everywhere. From large datacenters to mobile devices, an ever richer assortment of applications and services relies on distributed systems, infrastructure, and protocols. Despite their ubiquity, testing and debugging distributed systems remains notoriously hard. Moreover, aside from inherent design challenges posed by partial failure, concurrency, or asynchrony, there remain significant challenges in the implementation of distributed systems. These programming challenges stem from the increasing complexity of the concurrent activities and reactive behaviors in a distributed system on the one hand, and the need to effectively leverage the parallelism offered by modern multi-core hardware, on the other hand. This thesis contributes Kompics, a programming model designed to alleviate some of these challenges. Kompics is a component model and programming framework for building distributed systems by composing message-passing concurrent components. Systems built with Kompics leverage multi-core machines out of the box, and they can be dynamically reconfigured to support hot software upgrades. A simulation framework enables deterministic execution replay for debugging, testing, and reproducible behavior evaluation for large-scale Kompics distributed systems. The same system code is used for both simulation and production deployment, greatly simplifying the system development, testing, and debugging cycle. We highlight the architectural patterns and abstractions facilitated by Kompics through a case study of a non-trivial distributed key-value storage system. CATS is a scalable, fault-tolerant, elastic, and self-managing key-value store which trades off service availability for guarantees of atomic data consistency and tolerance to network partitions. We present the composition architecture for the numerous protocols employed by the CATS system, as well as our methodology for testing the correctness of key CATS algorithms using the Kompics simulation framework. Results from a comprehensive performance evaluation attest that CATS achieves its claimed properties and delivers a level of performance competitive with similar systems which provide only weaker consistency guarantees. More importantly, this testifies that Kompics admits efficient system implementations. Its use as a teaching framework as well as its use for rapid prototyping, development, and evaluation of a myriad of scalable distributed systems, both within and outside our research group, confirm the practicality of Kompics. / Kompics / CATS / REST

L'acquisition autonome de representations et de fonctionnalites en robotique pose de nombreux problemes theoriques. Aujourd'hui, les systemes robotiques autonomes sont concus autour d'un ensemble de fonctionnalites. Leurs representations du monde sont issues de l'analyse d'un probleme et d'une modelisation prealablement donnees par les concepteurs. Cette approche limite les capacites d'apprentissage. Nous proposons dans cette these un systeme ouvert de representations et de fonctionnalites. Ce systeme apprend en experimentant son environnement et est guide par l'augmentation d'une fonction de valeur. L'objectif du systeme consiste a agir sur son environnement pour reactiver les representations dont il avait appris une connotation positive. Une analyse de la capacite a generaliser la production d'actions appropriees pour ces reactivations conduit a definir un ensemble de proprietes necessaires pour un tel systeme. Le systeme de representation est constitue d'un reseau d'unites de traitement semblables et utilise un codage par position. Le sens de l'etat d'une unite depend de sa position dans le reseau. Ce systeme de representation possede des similitudes avec le principe de numeration par position. Une representation correspond a l'activation d'un ensemble d'unites. Ce systeme a ete implemente dans une suite logicielle appelee NeuSter qui permet de simuler des reseaux de plusieurs millions d'unites et milliard de connexions sur des grappes heterogenes de machines POSIX. Les premiers resultats permettent de valider les contraintes deduites de l'analyse. Un tel systeme permet d'apprendre dans un meme reseau, de facon hierarchique et non supervisee, des detecteurs de bords et de traits, de coins, de terminaisons de traits, de visages, de directions de mouvement, de rotations, d'expansions, et de phonemes. NeuSter apprend en ligne en utilisant uniquement les donnees de ses capteurs. Il a ete teste sur des robots mobiles pour l'apprentissage et le suivi d'objets.

Distributed systems are everywhere. From large datacenters to mobile devices, an ever richer assortment of applications and services relies on distributed systems, infrastructure, and protocols. Despite their ubiquity, testing and debugging distributed systems remains notoriously hard. Moreover, aside from inherent design challenges posed by partial failure, concurrency, or asynchrony, there remain significant challenges in the implementation of distributed systems. These programming challenges stem from the increasing complexity of the concurrent activities and reactive behaviors in a distributed system on the one hand, and the need to effectively leverage the parallelism offered by modern multi-core hardware, on the other hand. This thesis contributes Kompics, a programming model designed to alleviate some of these challenges. Kompics is a component model and programming framework for building distributed systems by composing message-passing concurrent components. Systems built with Kompics leverage multi-core machines out of the box, and they can be dynamically reconfigured to support hot software upgrades. A simulation framework enables deterministic execution replay for debugging, testing, and reproducible behavior evaluation for largescale Kompics distributed systems. The same system code is used for both simulation and production deployment, greatly simplifying the system development, testing, and debugging cycle. We highlight the architectural patterns and abstractions facilitated by Kompics through a case study of a non-trivial distributed key-value storage system. CATS is a scalable, fault-tolerant, elastic, and self-managing key-value store which trades off service availability for guarantees of atomic data consistency and tolerance to network partitions. We present the composition architecture for the numerous protocols employed by the CATS system, as well as our methodology for testing the correctness of key CATS algorithms using the Kompics simulation framework. Results from a comprehensive performance evaluation attest that CATS achieves its claimed properties and delivers a level of performance competitive with similar systems which provide only weaker consistency guarantees. More importantly, this testifies that Kompics admits efficient system implementations. Its use as a teaching framework as well as its use for rapid prototyping, development, and evaluation of a myriad of scalable distributed systems, both within and outside our research group, confirm the practicality of Kompics. / <p>QC 20130520</p>

La medicina regenerativa és una disciplina que ha guanyat reconeixement en les últimes dècades pel fet que moltes malalties no són tractables amb fàrmacs convencionals. Molts grups de recerca i empreses inverteixen temps i diners en la producció de nous paradigmes per curar malalties com el Parkinson, l'artrosi o la regeneració de medul·la espinal. Aquestes propostes es basen en l'ús de teixits biomimètics per reparar òrgans danyats. En aquesta tesi es presenta un nou model experimental per estudiar la formació d'os i cartílag i eventualment la reparació d'aquests teixits. Hem utilitzat Fibroblasts Embriònics de Ratolí (MEFs) combinats amb diferents materials biomimètics per estudiar os i cartílag in vitro i in vivo. Aquests MEFs es van cultivar in vitro i in vivo en RAD16-I, un pèptid auto-ensamblable amb estructura similar a matrius extracel·lulars genèriques, amb l'objectiu d'estudiar l'evolució dels fibroblasts en aquestes dues condicions. També s'han recobert superficialment micropartícules de hidroxiapatita obtenint càrregues inorgàniques similars a l'os i biològicament actives per a utilitzar-les com a substituts d'os o cartílag. Amb la idea de millorar els recobriments superficials, hem desenvolupat una plataforma que permet dur a terme proves combinatòries amb factors de creixement i altres compostos biològicament actius. Cultius in vitro de MEFs han mostrat que quan fibroblasts embrionaris primaris de ratolí es cultiven en RAD16-I, estableixen una xarxa intercel·lular que causa una contracció cel·lular organitzada, proliferació i migració cel·lulars i culmina amb la formació d'una estructura bilateral i simètrica amb un eix central distingible. Durant aquest procés morfològic, augmenta l'expressió d'un grup de gens mesodèrmics (brachyury, Sox9, Sox5, Sox6, Runx2). L'expressió de brachyury està localitzada primer en l'eix de simetria central i després s'extén als dos costats de l'estructura. Per acabar, la formació espontània d'un teixit similar al del cartílag acompanya l'expressió de Sox9 i Runx2.L'estudi in vivo de MEFs es va fer gràcies a la tècnica de presa d'imatges no invasiva basada en bioluminiscència (BLI) que ha desenvolupat en el grup de recerca del dr. Jerónimo Blanco. Aquests experiments han mostrat que el RAD16-I és una matriu molt permissiva per a la supervivència i proliferació cel·lulars in vivo. A més, sembla que les pobres propietats mecàniques que té el RAD16-I no li suposen cap desavantatge en termes de creixement cel·lular in vivo. Finalment, hem desenvolupat diferents tipus de micropartícules de hidroxiapatita (HA) no recobertes, i recobertes mitjançant polimerització assistida per plasma. Els recobriments permeten afinar les propietats de la HA i produir partícules que satisfacin les necessitats de diferents aplicacions mèdiques en reparació d'os i cartílag. També hem desenvolupat un mètode per produir plataformes basades en plaques de 96 pous que permetin fer proves combinatòries amb compostos biològicament actius per vàries aplicacions en medicina regenerativa. En conclusió, hem aportat noves idees i eines que permetran trobar teixits regeneratius basats en l'ús de fibroblasts i materials biomimètics. / La medicina regenerativa es una disciplina que ha ganado reconocimiento en las últimas décadas porque muchas enfermedades no son tratables con fármacos convencionales. Muchos grupos de investigación y empresas invierten tiempo y dinero en la producción de nuevos paradigmas para curar enfermedades como el Parkinson, la artrosis o la regeneración de médula espinal. Estas propuestas se basan en el uso de tejidos biomiméticos para reparar órganos dañados. En esta tesis se presenta un nuevo modelo experimental para estudiar la formación de hueso y cartílago y tal vez la reparación de estos tejidos. Hemos utilizado Fibroblastos Embrionarios de Ratón (MEFs) combinados con diferentes materiales biomiméticos para estudiar hueso y cartílago in vitro e in vivo. Estos MEFs se cultivaron in vitro e in vivo en RAD16-I, un péptido auto-ensamblable con estructura similar a matrices extracelulares genéricas, con el objetivo de estudiar la evolución de los fibroblastos en estas dos condiciones. También se han recubierto superficialmente micropartículas de hidroxiapatita obteniendo cargas inorgánicas similares al hueso y biológicamente activas para utilizarlas como sustitutos de hueso o cartílago. Con la idea de mejorar los recubrimientos superficiales, hemos desarrollado una plataforma que permite llevar a cabo pruebas combinatorias con factores de crecimiento y otros compuestos biológicamente activos. Cultivos in vitro de MEFs han mostrado que cuando fibroblastos embrionarios primarios de ratón se cultivan en RAD16-I, establecen una red intercelular que causa una contracción celular organizada, proliferación y migración celulares y culmina con la formación de una estructura bilateral y simétrica con un eje central distinguible. Durante este proceso morfológico, aumenta la expresión de un grupo de genes mesodérmicos (brachyury, Sox9, Sox5, Sox6, Runx2). La expresión de brachyury está localizada primero en el eje de simetría central y después se extiende a los dos lados de la estructura. Para terminar, la formación espontánea de un tejido similar al del cartílago acompaña a la expresión de Sox9 y Runx2.El estudio in vivo de MEFs se hizo gracias a la técnica de toma de imágenes no invasiva basada en bioluminiscencia (BLI) que han desarrollado en el grupo de investigación del dr. Jerónimo Blanco. Estos experimentos han mostrado que el RAD16-I es una matriz muy permisiva para a la supervivencia y proliferación celulares in vivo. Además, parece que las pobres propiedades mecánicas que tiene el RAD16-I no le suponen ninguna desventaja en términos de crecimiento celular in vivo. Finalmente, hemos desarrollado diferentes tipos de micropartículas de hidroxiapatita (HA) no recubiertas, y recubiertas mediante polimerización asistida por plasma. Los recubrimientos permiten afinar las propiedades de la HA y producir partículas que satisfagan las necesidades de diferentes aplicaciones médicas en reparación de hueso y cartílago. También hemos desarrollado un método para producir plataformas basadas en placas de 96 pozos que permitan hacer pruebas combinatorias con compuestos biológicamente activos para varias aplicaciones en medicina regenerativa. En conclusión, hemos aportado nuevas ideas y herramientas que permitirán hallar tejidos regenerativos basados en el uso de fibroblastos y materiales biomiméticos. / Regenerative medicine is a discipline that has gained recognition in the last decades because many diseases are not treatable with traditional drugs. Many research groups and companies invest time and money in the production of new paradigms to cure conditions such as Parkinson's, arthrosis or spinal cord injuries. These approaches are based in the use of biomimetic tissues to replace damaged organs. In this work we present a new experimental model to study the formation of bone and cartilage and eventually to repair these tissues. We have used Mouse Embryonic Fibroblasts (MEFs) combined with different biomimetic materials to study bone and cartilage formation in vitro and in vivo. MEFs have been cultured in vitro and in vivo in RAD16-I, a synthetic self-assembling peptide with structure similar to generic extracellular matrix milieu, to study the evolution of these fibroblasts in both conditions. Also, hydroxyapatite microparticles have been surface coated to produce biologically active bone-like inorganic charges for use in cartilage or bone substitutes. In order to improve the particles' coatings, we have developed a platform that allows us to perform combinatorial testing of growth factors and other biologically active compounds. In vitro cultures of MEFs has shown that when primary mouse embryonic fibroblasts are cultured in a soft nanofiber scaffold, they establish a cellular network that causes an organized cell contraction, proliferation, and migration that ends in the formation of a symmetrically bilateral structure with a distinct central axis. A subset of mesodermal genes (brachyury, Sox9, Sox5, Sox6, Runx2) is upregulated during this morphogenetic process. The expression of brachyury was localized first at the central axis, extending then to both sides of the structure. The spontaneous formation of cartilage-like tissue mainly at the paraxial zone followed the expression of Sox9 and Runx2.In vivo study of MEFs was facilitated by a non-invasive bioluminescence imaging (BLI) technique to detect luciferase-expressing cells, developed by Dr. Blanco's research group. These experiments showed that RAD16-I is a very permissive scaffold for cell survival and proliferation in vivo. Furthermore, it seems that the poor mechanical properties of RAD16-I are no disadvantage in terms of cell growth in vivo.Finally, we have developed different types of coated and uncoated hydroxyapatite (HA) microparticles by plasma polymerization. The coatings permit to tune the properties of HA and produce particles that suit the needs of different medical applications in bone and cartilage repair. Moreover, we have developed a method to produce platforms based on 96-well plates that allow the combinatorial testing of biologically active compounds for various applications in regenerative medicine. In conclusion, we have supplied new insights and tools that will enhance the finding of new regenerative tissues based on fibroblasts and biomimetic materials.

Aktive Filament-Systeme, wie zum Beispiel das Zellskelett, sind Beispiele einer interessanten Klasse neuartiger Materialien, die eine wichtige Rolle in der belebten Natur spielen. Viele wichtige Prozesse in lebenden Zellen wie zum Beispiel die Zellbewegung oder Zellteilung basieren auf dem Zellskelett. Das Zellskelett besteht aus Protein-Filamenten, molekularen Motoren und einer großen Zahl weiterer Proteine, die an die Filamente binden und diese zu einem Netz verbinden können. Die Filamente selber sind semifexible Polymere, typischerweise einige Mikrometer lang und bestehen aus einigen hundert bis tausend Untereinheiten, typischerweise Mono- oder Dimeren. Die Filamente sind strukturell polar, d.h. sie haben eine definierte Richtung, ähnlich einer Ratsche. Diese Polarität begründet unterschiedliche Polymerisierungs- und Depolymerisierungs-Eigenschaften der beiden Filamentenden und legt außerdem die Bewegungsrichtung molekularer Motoren fest. Die Polymerisation von Filamenten sowie Krafterzeugung und Bewegung molekularer Motoren sind aktive Prozesse, die kontinuierlich chemische Energie benötigen. Das Zellskelett ist somit ein aktives Gel, das sich fern vom thermodynamischen Gleichgewicht befindet. In dieser Arbeit präsentieren wir Beschreibungen solcher aktiven Filament-Systeme und wenden sie auf Strukturen an, die eine ähnliche Geometrie wie zellulare Strukturen haben. Beispiele solcher zellularer Strukturen sind Spannungsfasern, kontraktile Ringe oder mitotische Spindeln. Spannungsfasern sind für die Zellbewegung essentiell; sie können kontrahieren und so die Zelle vorwärts bewegen. Die mitotische Spindel trennt Kopien der Erbsubstanz DNS vor der eigentlichen Zellteilung. Der kontraktile Ring schließlich trennt die Zelle am Ende der Zellteilung. In unserer Theorie konzentrieren wir uns auf den Einfluß der Polymerisierung und Depolymerisierung von Filamenten auf die Dynamik dieser Strukturen. Wir zeigen, dass der kontinuierliche Umschlag (d.h. fortwährende Polymerisierung und Depolymerisierung) von Filamenten unabdingbar ist für die kontraktion eines Rings mit konstanter Geschwindigkeit, so wie in Experimenten mit Hefezellen beobachtet. Mit Hilfe einer mikroskopisch motivierten Beschreibung zeigen wir, wie &amp;quot;filament treadmilling&amp;quot;, also Filament Polymerisierung an einem Ende mit der gleichen Rate wie Depolymerisierung am anderen Ende, zur Spannung in Filament Bündeln und Ringen beitragen kann. Ein zentrales Ergebnis ist, dass die Depolymerisierung von Filamenten in Anwesenheit von filamentverbindenden Proteinen das Zusammenziehen dieser Bündel sogar in Abwesenheit molekulare Motoren herbeiführen kann. Ferner entwickeln wir eine generische Kontinuumsbeschreibung aktiver Filament-Systeme, die ausschließlich auf Symmetrien der Systeme beruht und von mikroskopischen Details unabhängig ist. Diese Theorie erlaubt uns eine komplementäre Sichtweise auf solche aktiven Filament-Systeme. Sie stellt ein wichtiges Werkzeug dar, um die physikalischen Mechanismen z.B. in Filamentbündeln aber auch bei der Bildung von Filamentringen im Zellkortex zu untersuchen. Schließlich entwickeln wir eine auf einem Kräftegleichgewicht basierende Beschreibung für bipolare Strukturen aktiver Filamente und wenden diese auf die mitotische Spindel an. Wir diskutieren Bedingungen für die Bildung und Stabilität von Spindeln. / Active filament systems such as the cell cytoskeleton represent an intriguing class of novel materials that play an important role in nature. The cytoskeleton for example provides the mechanical basis for many central processes in living cells, such as cell locomotion or cell division. It consists of protein filaments, molecular motors and a host of related proteins that can bind to and cross-link the filaments. The filaments themselves are semiflexible polymers that are typically several micrometers long and made of several hundreds to thousands of subunits. The filaments are structurally polar, i.e. they possess a directionality. This polarity causes the two distinct filament ends to exhibit different properties regarding polymerization and depolymerization and also defines the direction of movement of molecular motors. Filament polymerization as well as force generation and motion of molecular motors are active processes, that constantly use chemical energy. The cytoskeleton is thus an active gel, far from equilibrium. We present theories of such active filament systems and apply them to geometries reminiscent of structures in living cells such as stress fibers, contractile rings or mitotic spindles. Stress fibers are involved in cell locomotion and propel the cell forward, the mitotic spindle mechanically separates the duplicated sets of chromosomes prior to cell division and the contractile ring cleaves the cell during the final stages of cell division. In our theory, we focus in particular on the role of filament polymerization and depolymerization for the dynamics of these structures. Using a mean field description of active filament systems that is based on the microscopic processes of filaments and motors, we show how filament polymerization and depolymerization contribute to the tension in filament bundles and rings. We especially study filament treadmilling, an ubiquitous process in cells, in which one filament end grows at the same rate as the other one shrinks. A key result is that depolymerization of filaments in the presence of linking proteins can induce bundle contraction even in the absence of molecular motors. We extend this description and apply it to the mitotic spindle. Starting from force balance considerations we discuss conditions for spindle formation and stability. We find that motor binding to filament ends is essential for spindle formation. Furthermore we develop a generic continuum description that is based on symmetry considerations and independent of microscopic details. This theory allows us to present a complementary view on filament bundles, as well as to investigate physical mechanisms behind cell cortex dynamics and ring formation in the two dimensional geometry of a cylinder surface. Finally we present a phenomenological description for the dynamics of contractile rings that is based on the balance of forces generated by active processes in the ring with forces necessary to deform the cell. We find that filament turnover is essential for ring contraction with constant velocities such as observed in experiments with fission yeast.

本研究採用質性研究方法，以開放系統理論為架構，從組織運作的角度來探究個案學校─種籽親子實驗國民小學之日常生活和教育實踐，從而描繪出這所另類學校的學校圖像，並分析該校穩健存續的因素。本研究之研究結果如下： 一、本研究分別以「學生學習的制度」和「學校運作的制度」來描繪種籽親子實驗國民小學之學校圖像。前者包含課程學習及生活學習的各項制度，開展出師生自由自主、和諧共融的家校生活；後者敘述親師合作辦學治校，以及攸關教師成長的各項制度，開展出親師互信互賴、共同成長的家校生活。這兩個圖像共同呈現該校特殊的「家校生活」面貌：親師生皆能自在地以其本然面貌所展現的個別性，在學校共同生活。親師生和諧共融、互信互賴的基礎正在於鼓勵個人真實呈現自我的制度與文化。「家校生活」不僅意味著親師生在學校能延續著各自在家庭裡個人化的樣貌，更顯示家庭與學校之間資源互通、成員交流，學校呈現出由一個個學生家庭及教師團成員的家庭所組成的共同體。學校生活能自然融合、連結個人生活的其他面向，個別親師生因而在學校展現出一個個自主自在的完整學習個體的面貌。 二、種籽親子實驗國民小學的內部系統特性及其外部環境關係，如下數端： （一）結構系統呈現低度科層體制特性 （二）討論文化的底蘊是「支持個別差異的開放文化」與「有機體的合作文化」 （三）教師個人系統與家長、學生個人系統彼此協調而互有影響 （四）政治系統顯現個人範疇權力的運用與組織需求的轉化能解決衝突 （五）學校與地方教育主管機關互動良好但疏於經營與所在社區的關係 三、自我組織（self-organization）的組織本質是種籽親子實驗國民小學穩健存續的因素。以內部系統來看，該校的文化系統強韌並與個人系統協調，使其維持低度科層結構並得以化解組織衝突，內部系統顯現調和一致性。以學校組織的整體系統來看，該校是文化系統運作顯著的開放系統，文化系統具自主性的「調節回饋」本質，使學校形成「自我組織」，能因應內部系統與外部環境的擾動或壓力，得以穩健存續。 四、對於種籽親子實驗國民小學的建議： （一）親師生合力建構「種籽學」論述，做為自我改革學校變革的系統性基礎 （二）建立師資培育模式，踐行公共化使命 五、本研究對於國民教育的啟示： （一）朝向彈性的「自我組織」發展，學校更具創新變革能力 （二）建立有利於「自我創化教師」生成的學校環境 （三）親師生共同建構學校信念，形成「信任連結」關係為學校發展基礎 （四）以種籽親子實小的經驗看「實驗教育」的挑戰與因應 1. 「學校評鑑」方式應以協助學校發展為目的，宜更具彈性、因校制宜 2. 主管機關宜促進另類學校與一般學校深度交流或彼此成果分享 3. 種籽親子實驗國民小學的學校運作模式可為偏地小校轉型再生的參考 / This dissertation applies qualitative research method and the “open systems theory” to amplify on an alternative school picture of Seedling Experimental Elementary School, and inquiries the elements on the sustainable development of the alternative school. The results of this dissertation upon the case study on Seedling Experimental Elementary School are as follows. 1. By describing and analyzing the manners and systems of student learning and school operating, this dissertation points out that the school picture of Seedling Experimental Elementary School is home-school life of trustful coupling relationship among parents, teachers, and students. 2. The organizational characteristics of Seedling Experimental Elementary School are as below. (1) It seems as if Seedling Experimental Elementary School is a bureaucratic organization, but just at a modest rate, is not typical bureaucracy. (2) The intrinsic qualities of discussing culture are supporting individual differences and cooperation. (3) The individual systems of parents, teachers, and students are congruent. (4) Invoking individual power and transferring the need for organization can reconcile conflicts in the political system of organization. (5) Seedling Experimental Elementary School has the good relationship with the authorities, but has the aloof relationship with the local community. 3. The innate character of Seedling Experimental Elementary School is “self-organization” that can reconcile the inner systems of the school organization and embrace the challenges or stress come from outer systems. Seedling Experimental Elementary School as a self-organization can be developing steady. 4. This dissertation makes two suggestions to Seedling Experimental Elementary School. First of all, parents, teachers, and students can collectively build the “Seedling knowledge”. Secondly, Seedling Experimental Elementary School can accomplish educational mission for the public by establishing the method and program of teacher education based on “Seedling knowledge”. 5. This dissertation makes some suggestions for national education. (1) The authorities could assist schools to be “self-organization-orientated” school organizations. (2) The authorities could assist schools to cultivate teachers to be “self-creation-orientated” teachers. (3) A school principal could lead parents, teachers, and students to work up the school belief and vision, which may develop trustful coupling relationship that could be the base of school. (4) Three suggestions for experimental education: first, school evaluation for schools which are proceeding with experimental education should be flexible and expediential; secondly, the authorities can boost the communication and promote the cooperation between alternative schools and common schools; finally, the school operating model of Seedling Experimental Elementary School could be the reference and resource for the transformation of small schools in rural.

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