Cette étude traite des propriétés optiques non linéaires (NL) du troisième ordre des tétraphénylporphyrines et de certains de ses dérivées métallisées (Zn, Co) dissoutes dans le chlorobenzène. Les solutions ont été exposées à un laser émettant à 1064 nm, 532 nm et 355 nm dans le régime picoseconde ( ≈ 10 ps) en utilisant la méthode D4σ-Z-scan dans un montage 4f ainsi qu’une nouvelle technique appelée Dark-Field Zscan. Cette dernière se révèle être très fiable pour la détermination directe du signal de réfraction non linéaire en présence d’une forte absorption linéaire et/ou non linéaire. Le comportement des coefficients non linéaires a été étudié en fonction de la concentration et de l’intensité laser. La réponse a été attribuée à une susceptibilité effective du 3eme ordre {χ} rsub {eff} rsup {(3)} = {χ} ^ {(1)} : {χ} ^ {(1)} , dominée par un processus en cascade. Il a été mis en évidence que le comportement optique non linéaire des porphyrines dépend fortement du métal incorporé et de la durée des impulsions par rapport aux durées de vie des états excités. Ces résultats démontrent aussi que l’extension des systèmes électroniques π -conjugués accroît les non linéarités du troisième ordre de ces complexes, ce qui devrait être utile à l’élaboration de nouveaux matériaux. Cette propriété se révèle être intéressante pour la synthèse de porphyrines plus complexes avec d’autres substituants. Les effets importants d'absorption et de réfaction NL donnent aux porphyrines un fort potentiel d’utilisation pour la limitation optique, la commutation optique et pour différents autres applications en optique non linéaire. / This study deals with the third-order nonlinear optical properties (NL) of tetraphenylporphyrins and some of its metallic derivatives (Zn, Co) dissolved in chlorobenzene. The solutions were exposed to a laser emitting at 1064 nm, 532 nm and 355 nm in the picosecond regime ( ≈ 10 ps) using D4σ-Z-scan method in a 4f setup and a new technique called Dark-Field Zscan. The latter provides to be very reliable for the direct determination of the nonlinear refractive signal in the presence of a strong linear absorption and / or nonlinear one. The behavior of nonlinear coefficients has been studied as a function of the concentration and laser intensity. The response has been attributed to an effective 3rd order susceptibility, {χ} rsub {eff} rsup {(3)} = {χ} ^ {(1)} : {χ} ^ {(1)} , dominated by a cascading process. It has been demonstrated that the nonlinear optical behavior of porphyrins strongly depends on the metal incorporated and the pulse duration when compared to lifetimes of the excited states. These results also demonstrate that the extension of π- conjugated electronic systems increases the third order nonlinearities of these complexes, which should be useful in the development of new materials. This property appears to be interesting for the synthesis of more complex porphyrins with other substituents. The high impact on the coefficients related to the NL absorption and refraction gives porphyrins great potential of use for applications in optical limiting, optical switching

Denna studie utgår från en tes om framgångsrik undervisning som baseras på Hatties m.fl. (2017) och Grevholms (2012) forskning. Uppsatsen är en jämförande studie som utgår från tre undervisningsmodeller i matematik, traditionell undervisningsmodell, montessorimodellen och singaporemodellen. Fokus är på matematikundervisning i årskurs 1-3, svensk skola. Studien omfattar dels en mindre litteraturstudie, dels en intervjustudie med tre lärare som arbetar enligt de tre modellerna. Jag utgår från ett sociokulturellt perspektiv på lärande, undervisning och kunskap. Analyserna baseras på en riktad kvalitativ innehållsanalys. Resultatet visar på för och nackdelar med de tre undervisningsmodellerna och hur väl de uppfyller kraven enligt tesen för en framgångsrik undervisning. I resultatet tydliggörs genom lärarnas utsagor problematiken med de olika undervisningsmodellerna. / The proposal in this study define successful teaching from Hattie et al. (2017) and Grevholm (2012) research results. This is a comparative study with three teaching models in mathematics, Traditional model, Montessori model and Singapore model. The focus is on mathematics teaching in primary school, in Sweden.  The study embrace a minor literature study and an interview study with three teachers, who work according the three models in their teaching. I use a socio-cultural perspective on learning, teaching and knowledge. A directed qualitative content analyze is used to analyze both the literature and the interviews.  The results show the pros and cons of the three teaching models, according to the requirements of the proposed ideas of successful teaching. The results of the teachers´ statements clarify and problematize the differences between the three educational models in practice.

The demand for today's material resources for energy storage is rapidly increasing and can result in both environmental and political conflicts that can affect the development of electronic devices due to high prices and limitations of raw materials for batteries. In this study, potential future composite electrodes were synthesised with an ex-situ approach by compositing redox-active 2,6-Diaminoanthraquinone and 1,3,5-Triformylphloroglucinol covalent organic framework (DAAQ-TFP COF) with conductive delaminated Ti3C2Tx MXene to maximise the number of redox-active moieties during cycling. In addition, solvothermal synthesis with the implementation of mechanical grinding as an exfoliation method was used to try to obtain DAAQ-TFP nanosheets to increase both the contact area between the two materials and the number of charge carriers. The sample was analysed with PXRD and BET surface analysis to characterise the crystallinity meanwhile SEM was utilised to study the morphology of the COF and the composite material. The specific capacitance of each electrode was estimated by cyclic voltammetry. The study showed a decrease in reduced specific capacitance with lower MXene content. Hence, this concludes pure Ti3C2Tx sheets have the highest capacitance contribution with a value of 48.79 Fg-1 meanwhile the composite electrode with a ratio of 1:1 was estimated to 32.26 Fg-1 with 0.0928 % of its moieties undergoing a redox reaction. A reduced capacitance with an increased COF-MXene ratio indicates that MXene contributes with more capacity relative to the COF, in combination with a non-successful exfoliation of DAAQ-TFP to single-layered nanosheets, reducing the interactions between the two materials.

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Made available in DSpace on 2014-07-29T15:16:35Z (GMT). No. of bitstreams: 1 patricia de sousa.pdf: 7071029 bytes, checksum: 82c8213b4442879d206467555a97c3c4 (MD5) Previous issue date: 2010-01-29 / Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis (PCM), a systemic disorder geographically restricted to Central and South America, and one of the most important endemic mycoses in these regions, especially among the male rural populations. The disease is most likely caused by the inhalation of asexual spores (conidia) produced by the mycelia form of the fungus, propagules that once in the lungs undergo differentiation towards the parasitic yeast form. The cell wall of P. brasiliensis is a dynamic structure, essentially composed of branched glucan (β-1,3 and β-1,6 glucans), chitin, lipids and mannoproteins. Many enzymes are responsible for cell wall remodeling. One of them is the Beta-1,3- glucanosyltransferase 2 (PbGel2p) presented here. The amino acid deduced sequence of PbGel2p presented similarity to others proteins involved in fungal cell wall biosynthesis and morphogenesis and it was characterized as a member of GH72 family, GH72_ subfamily. The recombinant rPbGel2p was overexpressed in Escherichia coli and the polyclonal antibody was obtained. The PbGel2p mRNA, as well as the protein, were detected at the highest level in the mycelium phase. The potencial role of PbGel2p in cell wall biosynthesis and morphogenesis was analyzed by assessing its ability to rescue the phenotype of the Saccharomyces cerevisiae GAS1Δ. The results indicated that PbGel2p is a cell wall-associated protein that probably works as a β-1,3-glucan elongase capable of mediating fungal cell wall integrity. In addition, predicted protein-protein interactions between PbGel2p and others proteins of the fungus P. brasiliensis were assessed by using a S. cerevisiae two hybrid system and pull-down assay. The proteins that were found to interact with PbGel2p are: anthranilate synthase component 2 (involved in the tryptophan pathway), MYND domain protein SamB (related to fungal morphogenesis), mitotic spindle checkpoint protein Mad2B (required of the organization of the cytoskeleton and control of cell cycle), G protein complex beta subunit CpcB (organize the cytoskeleton of actin), WD repeat protein (involved in the control of cell cycle), phosphatidylinositol-4-phosphate-5-kinase its3 (required of the organization of the actin cytoskeleton), Hsp60 (related of stress conditions like temperature) and ATPase (localized in the plasma membrane / involved in the glucose metabolism). This suggested the relation of PbGel2p in other process to maintenance of cell wall integrity. / Paracoccidioides brasiliensis é o agente causador da paracoccidioidomicose (PCM), prevalente em países da América Central e do Sul sendo considerada uma das mais importantes micoses endêmicas, especialmente entre a população rural masculina. A doença é causada pela inalação dos esporos assexuais (conídios) produzidos pela forma miceliana do fungo. Nos pulmões, os propágulos tendem à diferenciação para a forma leveduriforme parasitária. A parede celular de P. brasiliensis é uma estrutura dinâmica, composta essencialmente por glicanas ramificadas (ligações β-1,3 e β-1,6), quitina, lipídeos e manoproteínas. Muitas enzimas estão envolvidas no seu remodelamento. Uma delas é a Beta-1,3-glicanosiltransferase 2 (PbGel2p), apresentada aqui. A sequência deduzida de aminoácidos de PbGel2p mostrou similaridade com outras proteínas envolvidas na morfogênese e biossíntese da parede celular fúngica e foi caracterizada como parte da família GH72, subfamília GH72_ . A proteína recombinante rPbGel2p foi superexpressa em Escherichia coli e o anticorpo policlonal obtido. Os níveis de transcritos que codificam para PbGel2p, assim como os de proteína, foram detectados em maior teor na fase miceliana do fungo. O papel de PbGel2p na morfogênese e biossíntese da parede celular foi analisado pela habilidade da proteína em resgatar o fenótipo mutante para GAS1Δ de Saccharomyces cerevisiae. Os resultados indicaram que PbGel2p é uma proteína associada à parede celular que provavelmente atua no alongamento da β-1,3- glicana mediando a integridade da parede celular. Ainda, as interações proteína-proteína preditas entre PbGel2p e outras proteínas de P. brasiliensis usando o sistema de duplo híbrido e pull-down foram: componente 2 da antranilato sintase (envolvida na via do triptofano), proteína SamB com domínio MYND (relacionada com a morfogênese fúngica), proteína Mad2B (requerida na organização do citoesqueleto e controle do ciclo celular), subunidade beta da CpcB (organiza o citoesqueleto de actina), proteína com repetição WD (envolvida no controle do ciclo celular), fosfatidilinositol-4-fosfato-5-quinase (requerida para organização do citoesqueleto de actina), Hsp60 (relacionada à condições de estresse como temperatura) e ATPase (localizada na membrana plasmática/ involvida no metabolismo de glicose). Essas interações sugerem a relação de PbGel2p em outros processos celulares para manutenção da integridade da parede celular.

L’Ins(1,4,5)P3 joue un rôle évident dans la signalisation cellulaire :il permet la libération du Ca 2+ des stocks intracellulaires par son action au niveau de récepteurs spécifiques. Pour mettre fin à son action, l’Ins(1,4,5)P3 peut être dégradé par une Ins(1,4,5)P3 5-phosphatase en Ins(1,4)P2, un métabolite inactif. L’Ins(1,4,5)P3 peut aussi être transformé en Ins(1,3,4,5)P4 par une Ins(1,4,5)P3 3-kinase. L’Ins(1,3,4,5)P4 semble posséder des capacités de signalisation propres ou au contraire liées à celles de l’Ins(1,4,5)P3.<p>L’Ins(1,3,4,5)P4 est aussi le point de départ de toute une série d’inositol hautement phosphorylés, dont les rôles ne sont pas clairs. Trois isoformes de l’Ins(1,4,5)P3 3-kinase existent (A, B et C). Ces isoformes possèdent un domaine catalytique carboxy-terminal bien conservé. Par contre, les domaines amino-terminaux sont spécifiques et leur permettraient d’établir des interactions ou de subir des régulations propres. Pour tenter d’élucider le rôle fonctionnel de l’Ins(1,3,4,5)P4, nous avons généré et analysé des souris déficientes pour les isoformes B et C de cette enzyme.<p>Les souris déficientes pour l’Ins(1,4,5)P3 3-kinase C ne présentent pas de phénotype évident, ce qui suggère que son rôle n’est pas crucial ou que son absence peut être compensée par une autre enzyme.<p>Les souris déficientes pour l’Ins(1,4,5)P3 3-kinase B, par contre, présentent une immunodéficience caractérisée par une absence spécifique des lymphocytes T αβ périphériques. Cette absence fait suite à un blocage dans la différenciation du précurseur du lymphocyte, le thymocyte. Les caractéristiques de la signalisation induite par le récepteur de surface (TCR) permettent la sélection des thymocytes, de manière à constituer un pool de lymphocytes T restreints pour le MHC et tolérants pour le soi. Nous avons montré que ces phénomènes de sélection étaient défectueux dans les thymocytes mutants, du fait de leur hyporéactivité à la stimulation par le TCR. Le mécanisme responsable de cette hyporéactivité n’est pas encore élucidé. A première vue, la mobilisation de Ca 2+ ne semble pas altérée dans ces thymocytes mutants en réponse à des stimulations classiques. Cependant, d’autres types de stimulation, se rapprochant plus de celles réellement rencontrées par le thymocyte in vivo, doivent encore être investigués. L’intégrité d’autres voies de signalisation cruciales du lymphocyte T doit aussi être vérifiée.<p>En conclusion, l’isoforme B de l’Ins(1,4,5)P3 3-kinase et l’Ins(1,3,4,5)P4 qu’il produit jouent un rôle crucial dans la différenciation du thymocyte, par un mécanisme qui reste encore à déterminer. / Doctorat en sciences biomédicales / info:eu-repo/semantics/nonPublished

My primary argument in this thesis is that creation theologies significantly influenced early developments in the doctrine of the Trinity, especially in Augustine of Hippo’s theology. Thus this is a work of historical theology, but I conclude with proposals for how Augustine’s theologies of creation and the Trinity can be read fruitfully with modern theology. I critically analyse developments in trinitarian theologies in light of ideas that were held about creation. These include the doctrine of creation ‘out of nothing’ and ideas about other creative acts (e.g., forming or fashioning things). Irenaeus and other early theologians posited roles for God (the Father), the Word / Son, the Spirit, or Wisdom in creative acts without working out formal views on economic trinitarian acts. During the fourth century trinitarian controversies, creation ‘out of nothing’ and ideas about ‘modes of origin’ influenced thinking on consubstantiality and relations within the Trinity. Basil of Caesarea and others also presented ideas about trinitarian acts of creation and the Trinity in hexaemeral works. I will argue that in Augustine’s views of trinitarian acts of creation, he attributes roles to God (the Father), the Word / Son, and the Spirit. In his mature theology, he attributes the giving of formless existence, differentiated existence, and perfected existence to the three Persons respectively, while depicting shared roles. He also attributes to the Spirit the giving of the capability of ‘dynamic abiding’ to creatures, which gives them agency in continuing their existence. Augustine’s theologies of creation and the Trinity were significantly influenced by his exegesis of Gen. 1, John 1. 1-3, Wisdom, and other scriptures, and his ideas resonate with the hexaemeral works of Basil and Philo of Alexandria. I argue that scholars should examine these sources and Augustine’s own hexaemeral commentaries to gain a deeper understanding of his trinitarian theology.

During the race towards the4th generation (4G) cellular-based digital communication systems, a growth in the demand for high capacity, multi-media capable, improved Quality-of-Service (QoS) mobile communication systems have caused the developing mobile communications world to turn towards betterMultiple Access (MA) techniques, like Code Division Multiple Access (CDMA) [5]. The demand for higher throughput and better QoS in future 4G systems have also given rise to a scheme that is becoming ever more popular for use in these so-called ‘bandwidth-on-demand’ systems. This scheme is known as adaptive channel coding, and gives a system the ability to firstly sense changes in conditions, and secondly, to adapt to these changes, exploiting the fact that under good channel conditions, a very simple or even no channel coding scheme can be used for Forward Error Correction(FEC). This will ultimately result in better system throughput utilization. One such scheme, known as incremental redundancy, is already implemented in the Enhanced Data Rates for GSM Evolution (EDGE) standard. This study presents an extensive simulation study of a Multi-User (MU), adaptive channel coded Direct Sequence Spread Spectrum Multiple Access (DS/SSMA) communication system. This study firstly presents and utilizes a complex Base Band(BB) DS/SSMA transmitter model, aimed at user data diversity [6] in order to realize the MU input data to the system. This transmitter employs sophisticated double-sideband (DSB)Constant-Envelope Linearly Interpolated Root-of-Unity (CE-LI-RU) filtered General Chirp-Like (GCL) sequences [34, 37, 38] to band limit and spread user data. It then utilizes a fully user-definable, complex Multipath Fading Channel Simulator(MFCS), first presented by Staphorst [3], which is capable of reproducing all of the physical attributes of realistic mobile fading channels. Next, this study presents a matching DS/SSMA receiver structure that aims to optimally recover user data from the channel, ensuring the achievement of data diversity. In order to provide the basic channel coding functionality needed by the system of this study, three simple, but well-known channel coding schemes are investigated and employed. These are: binary Hamming (7,4,3) block code, (15,7,5) binary Bose-Chadhuri-Hocquenghem (BCH) block code and a rate 1/3 <i.Non-Systematic (NS) binary convolutional code [6]. The first step towards the realization of any adaptive channel coded system is the ability to measure channel conditions as fast as possible, without the loss of accuracy or inclusion of known data. In 1965, Gooding presented a paper in which he described a technique that measures communication conditions at the receiving end of a system through a device called a Performance Monitoring Unit (PMU) [12, 13]. This device accelerates the system’sBit Error Rate (BER) to a so-called Pseudo Error Rate(PER) through a process known as threshold modification. It then uses a simple PER extrapolation algorithm to estimate the system’s true BER with moderate accuracy and without the need for known data. This study extends the work of Gooding by applying his technique to the DS/SSMA system that utilizes a generic Soft-Output Viterbi Algorithm(SOVA) decoder [39] structure for the trellis decoding of the binary linear block codes [3, 41-50], as well as binary convolutional codes mentioned, over realistic MU frequency selective channel conditions. This application will grant the system the ability to sense changes in communication conditions through real-time BER measurement and, ultimately, to adapt to these changes by switching to different channel codes. Because no previous literature exists on this application, this work is considered novel. Extensive simulation results also investigate the linearity of the PER vs. modified threshold relationship for uncoded, as well as all coded cases. These simulations are all done for single, as well as multiple user systems. This study also provides extensive simulation results that investigate the calculation accuracy and speed advantages that Gooding’s technique possesses over that of the classic Monte-Carlo technique for BER estimation. These simulations also consider uncoded and coded cases, as well as single and multiple users. Finally, this study investigates the experimental real-time performance of the fully functional MU, adaptive coded, DS/SSMA communication system over varying channel conditions. During this part of the study, the channel conditions are varied over time, and the system’s adaptation (channel code switching) performance is observed through a real-time observation of the system’s estimated BER. This study also extends into cases with multiple system users. Since the adaptive coded system of this study does not require known data sequences (training sequences), inclusion of Gooding’s technique for real-time BER estimation through threshold modification and PER extrapolation in future 4G adaptive systems will enable better Quality-of-Service (QoS) management without sacrificing throughput. Furthermore, this study proves that when Gooding’s technique is applied to a coded system with a soft-output, it can be an effective technique for QoS monitoring, and should be considered in 4G systems of the future. / Dissertation (MEng (Computer Engineering))--University of Pretoria, 2007. / Electrical, Electronic and Computer Engineering / MEng / unrestricted

Syftet med detta examensarbete är att undersöka hur lärare med erfarenhet av Wittingmetoden beskriver sitt arbete med den samt om det på vetenskaplig grund går att argumentera för användning av metoden. Frågeställningarna är, om och varför lärare med erfarenhet av Wittingmetoden anser den vara lämplig för undervisning i läs- och skrivinlärning i årskurs 1–3 samt om det barn som redan kan läsa med ett visst flyt och förståelse i början av årskurs 1 också har nytta av metoden och i så fall hur. Frågeställningarna har uppstått när uppsatsförfattaren har ifrågasatt beskrivningar av metoden i kurslitteratur under lärarutbildningen. Beskrivningarna hävdar bland annat att metoden är så pass grundlig att den inte är relevant för undervisning av dagens elever då många av dem redan har en del förkunskaper. Underlag till studien utgörs dels av intervjuer med 10 lärare med utbildning i samt längre eller kortare erfarenhet av Wittingmetoden, dels av litteratur om Wittingmetoden, skriven av dess upphovskvinna Maja Witting samt tidigare forskning inom läs- och skrivinlärningsområdet kopplat till vissa karakteristiska delar av Wittingmetoden. Resultatet visar att Wittingmetoden ofta är inkorrekt beskriven samt att den förhåller sig mycket väl till vad den senaste forskningen menar är god och avgörande läs- och skrivundervisning i alfabetiska skriftspråk, nämligen ett arbetssätt med utgångpunkt i fonologisk medvetenhet och sammanljudning av fonem och grafem. Till detta ska sedan läggas olika arbetssätt för att skapa förutsättningar för god läsförståelse såsom utökat ordförråd, lärares högläsning, läsförståelsestrategier och allmänbildning. Sådan undervisning kan också, visar forskning, vara till nytta för den tidiga läsaren, särskilt det som gäller läsförståelse. Informanterna menar att Wittingmetoden är högst relevant och lämplig för läs- och skrivinlärning, liksom för ytterligare utveckling, för alla elever men att det finns lärare som den passar sämre för.