Global ETD Search

261	A reinterpretation of urban space in Pretoria Van der Klashorst, Elsa 02 1900 (has links) Various potential modes of interpreting the urban space in the inner city of Pretoria is evaluated in this study with the purpose of expanding discourse around spatial production in the city. Production of meaning through formal and structural means produced a city that served as administrative capital and ideological base for Afrikaners until the arrival of a democracy in 1994. The contemporary urban space is produced by people through everyday life, as theorised by Henry Lefebvre, rather than through formal means such as name changes. This study evaluates the way that identity and belonging is created by referring to everyday life practices, rhythmanalysis and daily activities as performances. Urban space is evaluated from a phenomenological perspective through the eyes of an artist and resident and expressed in an art exhibition. The way artists Julie Mehretu and Franz Ackermann dealt with urban space in their art is also referenced. / Art History, Visual Arts and Musicology / M.A. (Visual Arts) African city Afrikaner Art and city De Certeau Deleuze & Guattari Everyday life Lefebvre Performance Performativity Post-Apartheid city Post-colonial Pretoria Rhythmanalysis Social space Tshwane Urban geography Urban space 704.94468227 Public spaces in art Architecture -- South Africa -- Pretoria Cities and towns in art Pretoria (South Africa) -- In art Art, South African
262	Designing Cell-Free Protein Synthesis Systems for Improved Biocatalysis and On-Demand, Cost-Effective Biosensors Soltani Najafabadi, Mehran 06 August 2021 (has links) The open nature of Cell-Free Protein Synthesis (CFPS) systems has enabled flexible design, easy manipulation, and novel applications of protein engineering in therapeutic production, biocatalysis, and biosensors. This dissertation reports on three advances in the application of CFPS systems for 1) improving biocatalysis performance in industrial applications by site-specific covalent enzyme immobilization, 2) expressing and optimizing a difficult to express a mammalian protein in bacterial-based CFPS systems and its application for cost-effective, on-demand biosensors compatible with human body fluids, and 3) streamlining the procedure of an E. coli extract with built-in compatibility with human body fluid biosensors. Site-specific covalent immobilization stabilizes enzymes and facilitates recovery and reuse of enzymes which improves the net profit margin of industrial enzymes. Yet, the suitability of a given site on the enzyme for immobilization remains a trial-and-error procedure. This dissertation reports the reliability of several design heuristics and a coarse-grain molecular simulation in predicting the optimum sites for covalent immobilization of a target enzyme, TEM-1 ?-lactamase. This work demonstrates that the design heuristics can successfully identify a subset of favorable locations for experimental validation. This approach highlights the advantages of combining coarse-grain simulation and high-throughput experimentation using CFPS to efficiently identify optimal enzyme immobilization sites. Additionally, this dissertation reports high-yield soluble expression of a difficult-to-express protein (murine RNase Inhibitor or m-RI) in E. coli-lysate-based CFPS. Several factors including reaction temperature, reaction time, redox potential, and presence of folding chaperones in CFPS reactions were altered to find suitable conditions for m-RI expression. m-RI with the highest activity and stability was used to develop a lyophilized CFPS biosensor in human body fluids which reduced the cost of biosensor test by ~90%. Moreover, an E. coli extract with RNase inhibition activity was developed and tested which further streamlines the production of CFPS biosensors compatible with human body fluids. Mehran Soltani cell-free protein synthesis cell-free CFPS in vitro TEM-1 ?-lactamase antibiotics unnatural amino acid protein engineering site-specific covalent immobilization site-directed mutagenesis design heuristics coarse-grain simulation biosensor cell-free biosensor RNase inhibitor murine RNase inhibitor m-RI RNase inhibitor RNase A human body fluids saliva serum urine glutamine lyophilized point of care GroEL/ES folding chaperones E. coli extract Extract with RNase inhibition activity Engineering
263	Autonomie umění mimo instituce umění / Autonomy of an artwork outside of the institutions of art Hauser, Filip January 2022 (has links) This dissertation thesis aims to reflect on the current evolvement of public spaces and increasingly common discussions of the lay and professional public about its impact and importance for a democratic society. The main topic, "Autonomy of an artwork outside of the institutions of art", focuses on searching for the autonomy of artwork in public spaces in the context of how the artwork was placed there. The thesis describes specific artworks which were placed into the public space using different methods, such as art competition, no competition, as a gift, or in a way of temporary art. These methods are examined to find the degree of autonomy. The degree of autonomy of artworks in public spaces is determined based on the applied analysis which, among other things, focuses on how efficient the result is in comparison to the method of placement. The field research resulted in complex, subjective, sculptural and artistic opinion on the current practice of placement of artworks into public spaces. Such professional and relevant opinion was then presented to the responsible authorities which have a significant impact on the creation of public spaces. The author aims to apply the acquired knowledge in practice by offering his professional opinions for the new elaboration of the government strategic document called "Politika architektury a stavební kultury České republiky" (loosely translated as "Politics of Architecture and Building Culture of the Czech Republic"), which is created by Ministry of Regional Development of the Czech Republic. The main results of the thesis describe the efficient approaches which lead to cultivation of public spaces and the significant role of art in them. Such approaches were in the thesis demonstrated by civic influence on the government strategies or civic activism in a form of citizens' initiative called "Je to i tvoje město" (loosely translated as "The city is your too") which actively participates in the public debate in Jindřichův Hradec, town on the South of the Czech Republic.
264	Sites of Passage: Art as Action in Egypt and the US-- Creating an Autoethnography Through Performance Writing, Revolution, and Social Practice La Follette, Tavia 26 April 2013 (has links) No description available. Aesthetics Communication Cultural Anthropology Design Ecology Educational Theory Education Philosophy Epistemology Experiments Fine Arts International Relations Language Linguistics Middle Eastern Studies Metaphysics Multicultural Education Multimedia Communications Museums Peace Studies Pedagogy Performing Arts Spirituality Teaching Technical Communication Theater Theater Studies Egypt Revolution Occupy Movement Auto-ethnography Installation Art Performance Art Site Specific Theatre Theatre Studies Social Practice Social Justice Performance Writing Art Exchange Arab Spring 911 virtual website digital mother
265	Activation of Place : Curating Site-Specific Art at the Luleå Biennial in 2018 and 2020 / Aktivering av Plats : Curering av Platsspecifik Konst vid Luleåbiennalen 2018 och 2020 Nåtoft, Maria January 2023 (has links) This thesis investigates how site-specific art manifest itself in different ways, specifically in the biennial format. The focus is on the Luleå Biennale, one of Scandinavia's oldest biennales that returns every two years. Through a case study of four different works of art from two of the biennale's editions, two from the year 2018 and two from 2020, I want to broaden the understanding of the concept of site specificity and how to curate such an exhibition. The theory is based on Miwon Kwon's One Place to Another: Site-Specific Art and Locational identity (2004) and Nick Kaye's Site-Specific Art: Performance, Place and Documentation (2000) which deal with the concept of site specificity and its various expressions. The questions that arose were centered around research, audience and how art activates the place. Through my investigation, I came to the conclusion that all four artworks are strongly connected to their context, not only the context of the biennial but also the physical location they treated, and that a long process of research made the groundwork for this. One of the works was not newly produced for the place in which it was exhibited, but was realized by translating its original meaning to the place of the biennale with the help of curatorial work. I also come to the conclusion that the audience is of great importance in realizing these site-specific works of art exhibited in places that are otherwise not accessible to the public. The biennale opens up new places and memories. / Denna uppsats är en undersökning av hur platsspecifik konst kan uttrycka sig på olika sätt och specifikt hur det kan se ut i biennalformatet. Fokus ligger på Luleåbiennalen, en av Skandinaviens äldsta biennaler som återkommer vartannat år. Genom en fallstudie på fyra olika konstverk från två av biennalens utgåvor, två från år 2018 och två från 2020, vill jag vidga förståelsen för begreppet platsspecificitet och hur man curerar en sådan utställning. Den grundläggande teorin utgår från Miwon Kwons One Place to Another: Site-Specific Art and Locational identity (2004) och Nick Kayes Site-Specific Art: Performance, Place and Documentation (2000) som behandlar begreppet och dess olika uttryckssätt, vilket ledde till frågor kring research, publik och hur konsten aktiverar platsen. Genom min undersökning kommer jag fram till att alla fyra konstverk är starkt kopplade till sin kontext, inte endast kontexten av en biennal men även den fysiska platsen de behandlar, samt att en lång process av research har lagt grunden för detta. Ett av verken var inte tillverkade för platsen som den ställdes ut på men blev ändå realiserad genom att dess ursprungliga mening översattes till platsen för biennalen, detta med hjälp av curatoriskt arbete. Jag kommer även fram till att publiken utgör en viktig del för att realisera dessa platsspecifika konstverk som ställts ut på platser som annars inte är tillgängliga för allmänheten. Biennalen möjliggör besök av nya platser och minnen. Site specificity site-specific art site-related art Miwon Kwon Nick Kaye performance performance of space interdisciplinary contemporary art curating biennial art biennial Luleå Luleå biennial Norrbotten Karl Sjölund Raqs Media Collective Maria W Horn Kapwani Kiwanga Platsspecificitet platsspecifik konst plats-relaterad konst Miwon Kwon Nick Kaye performance performance of space interdisciplinärt samtidskonst curating biennial konstbiennial Luleåbiennialen Luleå Norrbotten Karl Sjölund Raqs Media Collective Maria W Horn Kapwani Kiwanga Humanities and the Arts Humaniora och konst Arts Konst Art History Konstvetenskap
266	The Secret Writer Chaffe, Tomas January 2013 (has links) This essay reflects a particular method and way of working that I employ when undertaking artistic research. My artworks are rooted and develop from the situation I find myself in as an artist, the very context I exhibit the work within. I do this by trying to understand this position, both on the micro and macro scale. As an artist currently studying at—and subsequently exhibiting in relation to— Konstfack, I base my research with the physical manifestation of the school. An imposing building that was part of a huge headquarters and factory site for the telecommunication company, Ericsson, in south Stockholm. The title of my essay is from the translation of a unique German cipher machine, the Geheimschreiber, made known to me through enquiry into this site. Throughout the Second World War the German army used this machine to send highly encrypted military messages across Swedish telephone cables. Following one of the greatest accomplishments in the history of cryptography, a Swedish mathematician broke this German code and subsequently assisted in designing a deciphering machine on behalf of the Swedish Intelligence branch. This device, known as the App, was secretly developed and manufactured by Ericsson, possibly where I now study. In exploring the theme of secrets, this essay originates from an underpinning desire and subject of my work to reveal what is concealed or overlooked. Through researching and writing this essay I attempt to have a better understanding on the notion of secrets, in both the private and public realms. Introducing the artistic process and situation I am working from, I explore the central role that secrets play within society. In order to understand secrecy today I introduce the intertwined and associated contemporary debates of privacy, (both private and public) and transparency through such subjects as Google’s new privacy policy, mobile phone hacking, WikiLeaks and offshore banking. secrets artistic research artwork context site specific telecommunication Ericsson germany second world war Geheimschreiber cryptography apps Swedish Intelligence reveal privacy private public transparency Google privacy policy mobile phone hacking WikiLeaks offshore banking FRA FRA-law Simmel Telefonplan Midsommarkransen Tomas Chaffe Thomas Chaffe LM Ericsson apparat Vigo Lindstein Arne Beurling Professor of Mathematics Uppsala LM-staden LM-city Försvarets Radioanstalt phonehacking Sissela Bok Georg Simmel Cypherpunk Micah Sifry Wikileaks and the Age of Transparency orwellian freedom contemporary art interactive telephone Visual Arts Bildkonst
267	Site-specific functionalization of antigen binding proteins for cellular delivery, imaging and target modulation Schumacher, Dominik 09 November 2017 (has links) Antikörper und Antigen-bindende Proteine, die an Fluorophore, Tracer und Wirkstoffe konjugiert sind, sind einzigartige Moleküle, welche die Entwicklung wertvoller diagnostischer und therapeutischer Werkzeuge ermöglichen. Allerdings ist der Konjugationsschritt sehr anspruchsvoll und trotz intensiver Forschung noch immer ein bedeutender Engpass. Zusätzlich sind Antigen-bindende Proteine oftmals nicht dazu in der Lage, die Zellmembran zu durchdringen und im Zellinneren nicht funktionsfähig. Daher ist ihre Verwendung auf extrazelluläre Targets beschränkt, was eine bedeutende Anzahl wichtiger Antigene vernachlässigt. Beide Limitierungen bilden Kernaspekte dieser Arbeit. Mit Tub-tag labeling wurde ein neuartiges und vielseitiges Verfahren für die ortsspezifische Funktionalisierung von Biomolekülen und Antigen-bindenden Proteinen entwickelt, und so die Palette der Proteinfunktionalisierungen bedeutend erweitert. Tub-tag wurde erfolgreich für die ortsspezifische Funktionalisierung verschiedener Proteine und Antigen-bindender Nanobodies angewendet, die für konfokale Mikroskopie, Proteinanreicherung und hochauflösende Mikroskopie eingesetzt wurden. In einem weiteren Projekt wurden zellpermeable Antigen-bindende Nanobodies hergestellt und somit das schon lange Zeit bestehende Ziel, intrazelluläre Targets durch in vitro funktionalisierte Antigen-bindende Proteine zu visualisieren und manipulieren, erreicht. Hierzu wurden zwei verschiedene Nanobodies an ihrem C-Terminus cyclischen zellpenetrierenden Peptiden unter Verwendung von Expressed Protein Ligation funktionalisiert. Diese Peptide ermöglichten die Endozytose-unabhängige Aufnahme der Nanobodies mit sofortiger Bioverfügbarkeit. Mit Tub-tag labeling und der Synthese von zellpermeablen Nanobodies konnten wichtige Bottlenecks im Bereich der Proteinfunktionalisierung und Antikörperforschung adressiert werden und neue Tools für die biochemische und zellbiologische Forschung entwickelt werden. / Antibodies and antigen binding proteins conjugated to fluorophores, tracers and drugs are powerful molecules that enabled the development of valuable diagnostic and therapeutic tools. However, the conjugation itself is highly challenging and despite intense research efforts remains a severe bottleneck. In addition to that, antibodies and antigen binding proteins are often not functional within cellular environments and unable to penetrate the cellular membrane. Therefore, their use is limited to extracellular targets leaving out a vast number of important antigens. Both limitations are core aspects of the presented thesis. With Tub-tag labeling, a novel and versatile method for the site-specific functionalization of biomolecules and antigen binding proteins was developed expanding the toolbox of protein functionalization. The method is based on the microtubule enzyme tubulin tyrosine ligase. Tub-tag labeling was successfully applied for the site-specific functionalization of different proteins including antigen binding nanobodies which enabled confocal microscopy, protein enrichment and super-resolution microscopy. In addition to that, cell permeable antigen binding nanobodies have been generated constituting a long thought goal of tracking and manipulating intracellular targets by in vitro functionalized antigen binding proteins. To achieve this goal, two different nanobodies were functionalized at their C-terminus with linear and cyclic cell-penetrating peptides using expressed protein ligation. These peptides triggered the endocytosis independent uptake of the nanobodies with immediate bioavailability. Taken together, Tub-tag labeling and the generation of cell-permeable antigen binding nanobodies strongly add to the functionalization of antibodies and their use in biochemistry, cell biology and beyond. Nanobodies Antigen-bindende Proteine Endozytose-unabhängige Zellaufnahme Ortsspezifische Funktionalisierung Tubulin Tyrosin Ligase Bioorthogonale Konjugation Manipulation intrazellulärer Antigene Protein-Protein-Interaktionen Antigen transport Nanobodies antigen binding proteins site-specific functionalization Tubulin Tyrosine Ligase bioorthogonal conjugation co-transport of antigens manipulation of intracellular antigens protein-protein interactions 572 Biochemie 547 Organische Chemie 570 Biologie WD 5085 WF 9900 VK 8567 ddc:572 ddc:547 ddc:540 ddc:570
268	Economics of nitrogen fertilization: Site-specific application, risk implications, and greenhouse gas emissions Karatay, Yusuf Nadi 18 February 2020 (has links) In Anbetracht des Kompromisses zwischen der Erzielung des höchsten Gewinns und der geringsten Umweltbelastung ist ein tiefes Verständnis der ökonomischen Folgen der Stickstoff (N) Düngung erforderlich. Die vorliegende Doktorarbeit liefert umfassende Einblicke in (i) die Auswirkungen des standortspezifischen N-Managements (SSNM) auf die Rentabilität und Risikominderung, (ii) die Auswirkungen von Unsicherheiten und Risikoeinflüssen auf optimale N-Düngergaben und (iii) das Potenzial und die Kosten der Vermeidung von Treibhausgas (THG) Emissionen durch N-Düngereduktion. Ein Modellierungsansatz wurde entwickelt, um die Wirkung von Ertrag und Proteingehalt, Wirtschafts- und Risikoauswirkungen sowie THG-Emissionen auf die N-Düngung zu simulieren. Die Ergebnisse der Arbeit zeigen, dass SSNM die Wirtschaftlichkeit verbessert, indem es eine höhere Weizenqualität und damit Preisprämien erzielt. SSNM reduziert das Risiko, die Backqualität nicht zu erreichen, und es gibt keine wesentlichen Nachteile beim Verlustrisikomanagement im Vergleich zum einheitlichen Management. Preisprämien für eine höhere Weizenqualität bieten Anreize für höhere N-Düngergaben. Prämien verflachen die Gewinnfunktion weiter, was unzureichende Argumente für eine Absenkung des N-Inputs aus der Wirtschaftlichkeitssicht liefert, selbst bei einer hohen Risikoaversion der Landwirte. Eine moderate Reduzierung der mineralischen N-Düngung kann die THG-Emissionen bei moderaten Opportunitätskosten mindern. Die THG-Vermeidung durch N-Düngereduktion in einer bestimmten Region kann unter Berücksichtigung kultur- und ertragszonenspezifischer Ertragswirkungen optimiert werden. Insgesamt liefert diese Arbeit wichtige Erkenntnisse über die Chancen und Nachteile der Anpassung der N-Düngergaben. Darüber hinaus leistet sie einen direkten Beitrag zur Identifizierung von kosten- und risikoeffizienten N-Managementoptionen und bildet die Grundlage für effektive politische Ansätze zur THG-Vermeidung durch selektive N-Düngereduktion. / Considering the tradeoff between achieving the highest profit and causing the lowest environmental impact, there is a need for a profound understanding of the economic consequences of nitrogen (N) fertilizer application. The present doctoral research provides comprehensive insights into (i) effects of site-specific N management (SSNM) on profitability and risk mitigation; (ii) impacts of uncertainties and risk implications on optimal N fertilizer rates; and (iii) potential and costs of mitigating greenhouse gas (GHG) emissions by N fertilizer reduction. A modelling approach was developed to simulate the response of yield, protein, economic and risk implications, and GHG emissions to N fertilizer application. Findings of the thesis show that SSNM improves profitability by achieving higher grain quality, thus, price premiums. SSNM reduces the risk of not reaching the baking grain quality and poses no considerable disadvantage on downside risk management compared to uniform management. Price premiums for higher wheat quality provide incentives for higher N input rates. Premiums further flatten the profit function, giving insufficient arguments for lowering N input from a farm profitability perspective, even in presence of high risk aversion of farmers. Moderate reduction of mineral N fertilizer can mitigate GHG emissions at moderate opportunity costs. GHG mitigation by N fertilizer reduction in a given region can be optimized considering crop and yield-zone-specific yield responses. Overall, this thesis provides important insights on chances and drawbacks of adjusting N fertilizer rates. Moreover, it makes a direct contribution in identifying cost- and risk-efficient N management options and provides a basis for effective policy approaches to reduce GHG emissions by selective N fertilizer reduction. Reaktiver Stickstoff Stickstoffdünger N-Mineralisation Ertragsfunktion Präzisionsackerbau teilflächenspezifische Düngung variable Düngung Ertragskartierung N-Sensor Wirtschaftlichkeit komparative Kostenvorteile Risikovermeidung Monte Carlo Simulation THG-Emissionen Klimawandel Vermeidungskosten Reactive nitrogen N fertilizer N mineralization yield response function precision farming site-specific application variable rate application yield mapping N sensor profitability comparative advantage risk mitigation Monte Carlo simulation GHG emissions climate change abatement costs ZB 92100 ZC 17800 ZD 69200 ddc:630
269	Beyond the electronic connection : the technologically manufactured cyber-human and its physical human counterpart in performance : a theory related to convergence identities Sharir, Yacov January 2013 (has links) This thesis is an investigation of the complex processes and relationships between the physical human performer and the technologically manufactured cyber-human counterpart. I acted as both researcher and the physical human performer, deeply engaged in the moment-to-moment creation of events unfolding within a shared virtual reality environment. As the primary instigator and activator of the cyber-human partner, I maintained a balance between the live and technological performance elements, prioritizing the production of content and meaning. By way of using practice as research, this thesis argues that in considering interactions between cyber-human and human performers, it is crucial to move beyond discussions of technology when considering interactions between cyber-humans and human performers to an analysis of emotional content, the powers of poetic imagery, the trust that is developed through sensory perception and the evocation of complex relationships. A theoretical model is constructed to describe the relationship between a cyber-human and a human performer in the five works created specifically for this thesis, which is not substantially different from that between human performers. Technological exploration allows for the observation and analysis of various relationships, furthering an expanded understanding of ‘movement as content’ beyond the electronic connection. Each of the works created for this research used new and innovative technologies, including virtual reality, multiple interactive systems, six generations of wearable computers, motion capture technology, high-end digital lighting projectors, various projection screens, smart electronically charged fabrics, multiple sensory sensitive devices and intelligent sensory charged alternative performance spaces. They were most often collaboratively created in order to augment all aspects of the performance and create the sense of community found in digital live dance performances/events. These works are identified as one continuous line of energy and discovery, each representing a slight variation on the premise that a working, caring, visceral and poetic content occurs beyond the technological tools. Consequently, a shift in the physical human’s psyche overwhelms the act of performance. Scholarship and reflection on the works have been integral to my creative process throughout. The goals of this thesis, the works created and the resulting methodologies are to investigate performance to heighten the multiple ways we experience and interact with the world. This maximizes connection and results in a highly interactive, improvisational, dynamic, non-linear, immediate, accessible, agential, reciprocal, emotional, visceral and transformative experience without boundaries between the virtual and physical for physical humans, cyborgs and cyber-humans alike. 700

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