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121	Characterisation of anandamide uptake in resting and activated murine cells Fredriksson Sundbom, Marcus January 2015 (has links) Modifying the metabolism of the body’s own endocannabinoids is a novel approach for analgesia. Two key catabolic enzymes are fatty acid amide hydrolase (FAAH) and inflammation-inducible cyclooxygenase 2 (COX-2). The cellular uptake of the key endocannabinoid anandamide (AEA) has been found to be regulated by its FAAH-catalysed intracellular degradation, but COX-2 has not been investigated in this respect. We aimed to find out whether or not COX-2 in an in vitro inflammation setting would be able to gate AEA uptake. To achieve this, C6 cells and Raw 264.7 cells were stimulated with LPS/INF-γ and lysates then analyzed by immunoblot in order to verify COX-2 expression. AEA cellular uptake was quantified using a radioassay with [3H]-AEA. It was found that COX-2 was not inducible in C6 cells using the LPS/INF-γ conditions studied, while it was inducible in Raw 264.7 cells. AEA uptake in the COX-2-induced Raw 264.7 cells was not reduced by inhibitors of this enzyme. FAAH appeared to be down-regulated in the stimulated Raw 264.7 cells, and this was reflected in an overall lower AEA uptake. Our interpretation of the data points to FAAH as gating AEA uptake. Additional experiments are required to validate our findings by verifying significance. Pharmacology and Toxicology Farmakologi och toxikologi
122	Preventing fatal effects of overworking : Product design solution Adawi, Rahim January 2018 (has links) “Overworking to death” is a phenomenon that has been noticeable in developing countries. The cause of death is mainly through ischemic strokes. While the victims’ occupations differed, they all shared a common characteristic, being positioned in a sedentary work, ranging from IT workers to doctors. This project’s aim was to develop a product that prevented or decreased the strokes that derived from sedentary overwork. This was mainly tackled by preventing one of the three causes of developing blood props, slowed blood flow. In order to gather rich data of the phenomenon, a qualitative study was conducted in China, during two months. By doing an extensive structured sampling, information rich data could be gathered during a short period of time. Data were derived from observations, questionnaires and an interview, which then was interpreted to customer needs and the final product specification. The final product became a trouser with an in built dynamic compression mechanic, that can compress the veins mostly during sitting activities, in order to prevent blood stasis. The compression mechanic works like the Chinese finger trap; compressing the calves while sitting and stretching the legs forward. It is made only out of polysaccharides fibres; cotton and corn. / "Guolaosi" eller död från överarbete är ett fenomen som i regel uppkommer bland utvecklingsländer. Dödsorsaken är huvudsakligen genom stroke. Offrens yrken varierar allt från professorer, IT-arbetare till läkare. De delar dock en sak gemensamt; att arbeta under långa perioder stillasittande. Projektets mål var att utveckla en produkt som minskar dödliga följderna av sedentära överarbete, genom att förebygga en av de tre orsakerna för att utveckla blodproppar; saktad blodström. Målgruppen var då kineser av de yrken som hade tidigare drabbats av fenomenet. För att samla informationsrika data om fenomenet genomfördes en kvalitativ studie i Kina under två månader. Genom att göra en omfattande strukturerad provtagning kunde informationsrika data samlas under en kort tidsperiod. Fältstudien bestod av observationer, frågeformulär och en intervju, som då tolkades till kundbehov och eventuellt produktspecifikationen. Den slutliga produkten kom att bli ett par byxor med en inbyggd dynamisk komprimeringsmekanism, som kan komprimera venerna under sittande aktiviteter, för att förhindra saktad blodström. Kompressionsmekanismen fungerar som den kinesiska fingerfällan. Den komprimerar blodkärlen medan personen sitter och sträcker benen framåt. Produkten är konstruerad på så sätt att den kan tillverkas endast av polysackariders tråd, från bomull och majs. Vilket är lämpligt för Kinas lokala resurser. stroke DVT overworking karoshi guolaosi prolonged inactivity sedentary death product design China Sweden Sida MFS field study trouser pants sampling observation questionnaire interview blood circulation stasis cardiovascular disease cotton corn agenda 2030 Chinese finger trap textile fibre yarn soil erosion muscle pump mechanism gradual compression stockings GECS PECS pressure distribution blood clot veins therapy stool chair PE PTS economy class syndrome methodology plastic sample concept sketch stroke DVT överarbete karoshi guolaosi förlängd inaktivitet stillasittande död produkt design Kina Sverige Sida MFS fältstudie byxa byxor provtagning observation frågeformulär intervju blodcirkulation stasis kardiovaskulär sjukdom bomull majs agenda 2030 kinesisk fingerfälla textil fiber garn jord erosion muskel pump mekanism gradvis komprimering strumpor stödstrumpa tryck kraft GECS PECS tryckfördelning blod blodproppar vener terapi pall stol PE PTS ekonomi klass syndrom metodik plast prov koncept skiss Design Design Pharmacology and Toxicology Farmakologi och toxikologi Occupational Therapy Arbetsterapi Cardiac and Cardiovascular Systems Kardiologi Environmental Sciences Miljövetenskap Medical Ergonomics Medicinsk ergonomi Textile, Rubber and Polymeric Materials Textil-, gummi- och polymermaterial Composite Science and Engineering Kompositmaterial och -teknik Medical Materials Medicinska material och protesteknik

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Characterisation of anandamide uptake in resting and activated murine cells

Preventing fatal effects of overworking : Product design solution