Made available in DSpace on 2014-06-11T19:27:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-02-25Bitstream added on 2014-06-13T18:56:53Z : No. of bitstreams: 1 andre_mr_me_jabo.pdf: 427316 bytes, checksum: 366b68e3f9cae8ab0938b9ab1032ffb9 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Poucos relatos têm sido feitos sobre o diagnóstico da erliquiose e babesiose em felinos domésticos e selvagens brasileiros, os quais são baseados diretamente pela presença de mórulas em leucócitos e piroplasmas em eritrócitos, e indiretamente pela detecção de anticorpos anti-Ehrlichia canis. O presente estudo teve como objetivo realizar a detecção molecular de E. canis e B. canis e a presença de anticorpos da classe IgG contra esses hemoparasitas em amostras de sangue e soro, respectivamente. Neste utilizamos 72 felídeos selvagens brasileiros mantidos em cativeiro em algumas instituições e zoológicos. Pela Reação de Imunofluorescência Indireta (RIFI), dezoito (25%) e cinqüenta e três (73,6%) dos 72 animais amostrados foram sororeagentes frente aos antígenos de E. canis e B. canis, respectivamente. Na PCR para E. canis, onze (15,3%) dos 72 animais amostrados foram positivos. Os amplicons foram confirmados por seqüenciamento e o DNA de E. canis encontrado mostrou grande similaridade genética com amostras de E. canis isoladas no Brasil, México, Portugal, Grécia e Taiwan, com 98% de similaridade. Nenhuma das amostras foi positiva na PCR para B. canis. Destaca-se a importância e a primeira detecção molecular de E. canis e presença de anticorpos anti-E. canis e anti-B. canis em felídeos selvagens brasileiros mantidos em cativeiro. / Few are the reports that have been carried out on ehrlichiosis and babesiosis diagnostic in Brazilian domestic and wild felids, which are based directly on the presence of morulae in leucocytes and piroplasms in erythrocytes, and indirectly by detection of antibodies against E. canis. The aim of this study was to detect molecularly E. canis and B. canis and the presence of anti-E. canis and B. canis IgG antibodies in the blood and sera samples, respectively, from 72 Brazilian wild captive felids maintained in some instituitions and zoos. Eighteen (25.0%) and fifty-three (73.6%) out of 72 animals were seroreagent for E. canis and B. canis antigen, respectively, by IFA (Indirect Immunofluorescent Assay). Eleven (15.3%) of the 72 samples were positive for nPCR E. canis. The amplicons were confirmed by sequencing and the E. canis DNA found appeared to be closely related to E. canis samples from Brazil, Mexico, Portugal, Greece and Taiwan with 98% percent identity. None of the 72 samples were positive for B. canis by PCR. This is the first molecular detection of E. canis and presence of seroreactivity for both B. canis and E. canis in Brazilian wild captive felids.

Made available in DSpace on 2014-06-11T19:27:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-12-14Bitstream added on 2014-06-13T19:36:23Z : No. of bitstreams: 1 sousa_kcm_me_jabo.pdf: 1190164 bytes, checksum: 4d98963d90176b0331075c9dd139f5bd (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Dentre as doenças emergentes causadas por artrópodes em cães destacam-se as infecções por agentes das espécies Leishmania chagasi, transmitida por flebotomíneos, Ehrlichia canis e Babesia canis transmitidas por carrapatos da espécie Rhipicephalus sanguineus. O presente estudo teve como objetivo realizar o diagnóstico sorológico e molecular de parasitos das espécies Leishmania chagasi, Ehrlichia canis e Babesia canis em amostras de soro e de baço, respectivamente. Foram utilizados 60 cães naturalmente infectados, eutanasiados no Centro de Controle de Zoonoses do Município de Campo Grande, Mato Grosso do Sul. Pela Reação de Imunofluorescência Indireta (RIFI), 60 (100%), 39 (65%) e 49 (81%) dos 60 animais amostrados foram sororeagentes frente aos antígenos de L.chagasi, E.canis e B.canis respectivamente. Também foi realizado o ensaio Imunoenzimático Indireto (ELISA-teste) para detecção de anticorpos da classe IgG anti-L. chagasi, onde sessenta cães (100%) mostraram-se soropositividade. Cinquenta e quatro cães (90%) mostraram-se positivos na reação em cadeia pela polimerase (PCR) para o gênero Leishmania sp e destes cinquenta e três animais, 53 (88%) foram positivos para o complexo L.Donovani. Vinte e sete animais (45%) foram positivos para Ehrlichia e destes animais todos foram positivos para E.canis pelo nested PCR e 2 (3,33%) cães foram PCR positivos para Babesia sp. Os amplicons foram confirmados por seqüenciamento e os DNAs mostraram 99% de similaridade genética para L.chagasi, E.canis e B.canis. Este estudo mostrou que os cães residentes em Campo Grande/MS, podem albergar mais de um hemoparasita, As co-infecções por agentes patogênicos intracelulares de células do sistema monocítico fagocitário, como é o caso da Ehrlichia e Leishmania, associadas ou não a Babesia... / Among emerging diseases caused by arthropods in dogs stand out infections by agents of the species Leishmania chagasi, transmitted by sandflies, Ehrlichia canis and Babesia canis transmitted by ticks of the species Rhipicephalus sanguineus. The present study aims to perform serological and molecular diagnosis of parasites of the species Leishmania chagasi, Ehrlichia canis and Babesia canis in samples of sera and spleen, respectively. A total of 60 naturally infected dogs were euthanized at the Zoonosis Control Center of the city of Campo Grande, Mato Grosso do Sul Through Immunofluorescence Assay (IFA), sixty (100%), 39 (65%) and 49 (81%) of the 60 animals sampled were reactive serum against the antigens L.chagasi, E.canis and B. canis, respectively. We also performed indirect immunosorbent assay (ELISA test) for detection of IgG antibodies anti-L. chagasi, where sixty dogs (100%) were seropositive. Fifty-four dogs (90%) were positive by PCR for the genus Leishmania, and of these fifty and three animals, 53 (88%) were positive for the complex L.Donovani. Twenty-seven dogs (45%) were positive for Ehrlichia sp and these animals were all positive for E.canis by nested PCR and two dogs (3%) were PCR positive for Babesia sp. The amplicons were confirmed by DNA sequencing and showed 99% genetic similarity to L.chagasi, E.canis and B. canis. This study showed that dogs living in Campo Grande / MS, can accommodate more than one haemoparasite. The co-infections by intracellular pathogens monocytic phagocyte system cells, as is the case with Ehrlichia and Leishmania, associated or not with Babesia (which multiplies in red blood cells), causing serious damage to the health of animals. The decline in physical condition and immune co-infections resulting from increases parasitemia, which in the case of agents transmitted by... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:27:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-31Bitstream added on 2014-06-13T19:15:43Z : No. of bitstreams: 1 kawanami_ae_me_jabo.pdf: 824530 bytes, checksum: e2cb2bd3bc907bc0688a009fdfc6fefd (MD5) / Os cervídeos têm sido acometidos por doenças hemorrágicas virais, tais como, doença epizoótica hemorrágica (DEH), língua azul (LA) e doença hemorrágica por adenovírus (DHA). Como as lesões macroscópicas, entre elas, enterite hemorrágica, edema pulmonar, petéquias e sufusões em diversos órgãos, são observadas nas três doenças, há necessidade de técnicas acuradas para realizar o diagnóstico definitivo. A partir do material de arquivo (blocos de parafina) existente no Departamento de Patologia Veterinária da FCAV – Unesp, 42 cervídeos brasileiros, tanto de vida livre como de cativeiro, foram selecionados, por apresentarem sinais clínicos e/ou lesões macroscópicas sugestivas de doenças virais hemorrágicas. Das amostras analisadas, utilizando técnica de imunoistoquímica, todas apresentaram resultado negativo para adenovírus. Utilizando técnica de RT-PCR em tempo real para vírus da doença epizoótica hemorrágica, os resultados foram também negativos. A mesma técnica aplicada para vírus da língua azul revelou sete animais positivos (16,66%) confirmados com eletroforese em gel de agarose 4% e sequenciamento. Todos os casos positivos foram de animais provenientes de cativeiro, sendo três fêmeas (duas jovens, uma adulta) e quatro machos jovens. As principais alterações macroscópicas observadas nesses animais foram conteúdo intestinal hemorrágico, mucosas avermelhadas do trato gastrointestinal, úlceras em língua e petéquias em diversos órgãos. Na histologia observou-se principalmente infiltrado inflamatório, hemorragia e congestão em diversos órgãos. Os vírus da DHA e DEH não estão envolvidos nos óbitos dos cervídeos estudados. A relevância deste trabalho está no fato de ser a primeira descrição de material genético do vírus da LA em cervídeos brasileiros / Cervids have been affected by viral hemorrhagic diseases, such as Epizootic hemorrhagic disease (EHD), Bluetongue (BT), and Adenoviral hemorrhagic disease (AHD). Once that gross lesions, among them, hemorrhagic enteritis, pulmonary edema, petechiae and suffusions in several organs, are similar, it is necessary to use accurate techniques to the definitive diagnosis. From the archival material (paraffin blocks) available in the Department of Veterinary Pathology of FCAV – Unesp, 42 Brazilian deer, both free living and captive, were selected because they had lesions suggestive of hemorrhagic viral disease. The samples analyzed, using Immunohistochemistry, were all negative for adenovirus. Using real time RT-PCR for EHD, the results were also negative. The same technique applied to BT virus revealed seven positive animals (16,66%) confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in these animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Mostly histological changes observed were inflammatory infiltrate, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. The AHD and EHD virus are not involved in the deaths of the deers studied in this research. The significance of this study is due to the fact that it was the first time the genome of BT virus was identified in Brazilian cervids

Made available in DSpace on 2014-06-11T19:23:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-06Bitstream added on 2014-06-13T19:49:57Z : No. of bitstreams: 1 oliveira_fm_me_araca.pdf: 788257 bytes, checksum: 350efb1f930e09b50897c9123c540f6f (MD5) / A leishmaniose visceral é uma doença em franca expansão no Brasil e que tem a participação de algumas espécies animais na manutenção de seu ciclo. Este trabalho objetivou investigar a presença de Leishmania spp. em morcegos de área endêmica para leishmaniose visceral e investigar a participação destes animais como possíveis reservatórios desta zoonose. Avaliou-se a presença de DNA dos parasitas, por meio da PCR, em amostras de baço e pele de 375 quirópteros originários de 19 cidades do Estado de São Paulo, Brasil, áreas endêmicas e com transmissão intensa para leishmaniose visceral. A amplificação de kDNA de Leishmania spp. por PCR foi positiva em 4,53% dos quirópteros, sendo que a maior positividade foi observada em amostras de baço (94,1%) e dois animais foram positivos tanto para amostras de pele como de baço; a presença de DNA do gene SSU 18S rRNA de Trypanosoma spp., também investigada, foi positiva em 4,53% das amostras sendo 23,5% positivas somente na amostra de baço, 76,5% somente na amostra de pele. Um animal apresentou positividade tanto para Leishmania spp. como para Trypanosoma spp. Os resultados indicam a ocorrência de Leishmania spp. em quirópteros da área endêmica para leishmaniose visceral, indicando a possibilidade de serem reservatórios desta zoonose / Visceral leishmaniasis is a disease with increasing incidence in Brazil and that has some animal species contributing to its maintenance. This study aimed at investigating the presence of Leishmania spp. in bats from an endemic area for visceral leishmaniasis, in order to assess its participation as possible potential reservoirs for this zoonosis. We collected spleen and skin samples of 375 bats from 19 cities in the State São Paulo, Brazil, areas of intense visceral leishmaniasis transmission. kDNA Leishmania ssp. amplification by PCR was positive in 4.53% bats and a higher positivity was seen in spleen samples (94.1%); two bats were positive for both skin and spleen samples. Trypanosoma spp. genomic DNA amplification was also investigated and 4.53% of the samples were positive, 23.5% of these were positive only for spleen, 76.5% for skin. One bat was positive for both Leishmania spp. and Trypanosoma spp. DNA amplification. These results demonstrate the occurrence of Leishmania spp. in bats from endemic area for visceral leishmaniasis, indicating the possibility of these being reservoirs of this zoonosis

Made available in DSpace on 2014-06-11T19:23:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-04-23Bitstream added on 2014-06-13T19:29:09Z : No. of bitstreams: 1 gouveia_gm_me_araca.pdf: 550439 bytes, checksum: 076629ee6a296748de89f110784f058d (MD5) / Os tumores mamários são as mais freqüentes neoplasias em cadelas, representando cerca de 50% dos tumores diagnosticadas nesta espécie e tem maior incidência em fêmeas de meia idade a idosas. Têm sido utilizadas como modelo de estudo para neoplasias mamárias de mulher devido às várias características comuns como as similaridades histológicas e imuno- histoquímicas. Na última década, estudos baseados nos perfis moleculares das neoplasias mamárias, tornaram possível a identificação de algumas células neoplásicas com características de células tronco – as células tronco tumorais (CTT). Uma das moléculas putativas para marcação das CTTs é o CD44. A telomerase, que vem sendo relatada como marcador tumoral, é potencialmente expressa também em CTTs. Estudos recentes estabeleceram um elo crucial entre a transição epitelial-mesenquimal (EMT) e a aquisição de propriedades moleculares e funcionais de células-tronco. Por este motivo analisamos a expressão das proteínas CD44, Citoqueratinas AE1/AE3 e Vimentina, e da enzima telomerase, em tumores mamários de cadelas a fim de investigar o potencial de marcação de CTTs e sua relação com a EMT. Para tal foram utilizados métodos de imuno-histoquímica em tecidos parafinizados tanto in vivo quanto in vitro, fazendo-se uso de técnicas como Tissue MicroArrays (TMA), citoinclusão de tecidos tumorais e cultivos celulares. A expressão da telomerase nas células de tumores mamários cultivadas foi analizada por PCR em tempo real. Nas amostras de tecido parafinizado in vivo, a imunomarcação da citoqueratina demonstrou diferença significativa entre tumores benignos e malignos (p˂0,05), mostrando-se mais intensa em tumores malignos. Já a vimentina apresentou maior intensidade de marcação em tumores benignos, porém não apresentando diferença significativa (p˂0,05)... / Mammary tumors are the most frequent cancers in dogs, representing about 50% of tumors, and have a higher incidence in females of middle aged and elderly. These tumors have been used as a model for breast cancer in women due to several common characteristics such as histological and immunohistochemical similarities. In the last decade, studies based on molecular profiles of breast cancer, made possible the identification of some neoplastic cells with characteristics of stem cells – cancer stem cells (CSC). One of the putative molecules of CSCs is CD44. Telomerase, which has been reported as a tumor marker, is potentially also expressed in CSCs. Recent studies have established a crucial link between the epithelial- mesenchymal transition (EMT) and the acquisition of molecular and functional properties of stem cells. For that reason we analyzed the expression of proteins CD44, Cytokeratins AE1/AE3 and Vimentin, and the telomerase enzyme, in dogs mammary tumors, to investigate the potencial for CSC markers, and its relation with the EMT using immunohistochemistry in paraffin embedded tissues in both in vivo and in vitro, making use of techniques such as Tissue MicroArrays (TMA), cell blocks from tumors and cell cultures. Besides that, we analyzed the telomerase expression in mammary tumor cultivated cells by quantitative real time PCR. Immunostaining of cytokeratin had no significant difference between benign and malignant tumors (p ˂ 0,05), being more intense in malignant tumors. However vimentina showed higher staining intensity in benign tumors, but with no significant difference (p ˂ 0,05). The expression of CD44 was higher in malignant tumors that have greater proliferative and metastatic potencial, however its relation with EMT was not detected in the analyzed tumors. Telomerase expression was positive in the neoplastic cultivated cells, however without significant difference... (Complete abstract click electronic access below)

Made available in DSpace on 2016-06-02T19:22:03Z (GMT). No. of bitstreams: 1 2193.pdf: 1048976 bytes, checksum: 7425ebc2647d9f4d59a0843f3908283e (MD5) Previous issue date: 2008-10-12 / Universidade Federal de Minas Gerais / Many studies have demonstrated that infection by Bartonella vinsonni subspecies berkhoffii may cause cardiac arrhythmia, myocarditis and infectious endocarditis in humans. There is strong evidence that Bartonella vinsonni is an important cardiac pathogen in both humans and dogs The Bartonella sp has been associated with several human diseases, being responsible for a number of zoonoses. The aim of this work was to identify and correlate the Bartonella vinsonni subsp. berkhoffii with the cardiac disease by using specific oligonucleotides as Bh16SF and Bh16SR, which amplify a 185-bp fragment of 16S rRNA. The PCR technique was used for detection of this bacterium in human leukocytes. The amplification products were analyzed in 1.5% agarose gel in order to confirm the 185-bp fragment, which in turn indicates the presence of the bacterium. Thirty eight (39%) out of the 96 humans with cardiopathies as well as 16 (15%) out of the 104 humans from the control group showed positive PCR amplification for Bartonella vinsonni subsp. berkhoffii, thus suggesting a positive correlation of this subspecies with the cardiopathies. Sequencing of the amplified product confirmed the presence of Bartonella vinsonni subspecies berkhoffii. / Estudos comprovam a existência de casos de arritmia cardíaca, miocardite e endocardite infecciosa causados por infecção pela bactéria Bartonella vinsonni subespécie berkhoffii em humanos. Há uma grande evidência que a espécie da Bartonella vinsonni é um patógeno cardíaco importante em humanos e cães. A Bartonella sp está associada a uma variedade de doenças humanas, sendo considerado uma zoonose. O objetivo deste trabalho foi identificar e correlacionar a bactéria Bartonella vinsonni subespécie berkhoffii com a patologia cardíaca utilizando oligonucleotídeos específicos Bh16SF e Bh16SR, que amplificam um segmento de 185 pb para o gene 16SrRNA e averiguar, por PCR, a presença de bactérias em leucócitos humanos. Os produtos de amplificação foram analisados em gel de agarose 1,5% para confirmação da amplificação do fragmento de DNA de 185 pb que indica a presença da bactéria. Trinta e oito (39%) dos 96 humanos cardiopatas e dezesseis (15%) dos 104 humanos do grupo controle apresentaram amplificação positiva por PCR para a bactéria Bartonella vinsonni subespécie berkhoffii, sugerindo uma correlação entre a bactéria e a cardiopatia. Análise por sequenciamento do material amplificado confirmou a presença da Bartonella vinsonni subespécie berkhoffii. Estes achados confirmam a existência de uma correlação entre a presença da bactéria Bartonella vinsonni subespécie berkhoffii e problemas cardíacos em humanos.

Made available in DSpace on 2014-06-11T19:23:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-03-07Bitstream added on 2014-06-13T18:19:52Z : No. of bitstreams: 1 fernandes_sc_me_jabo.pdf: 542693 bytes, checksum: f141575a6f8b47f554e2e0bf4870259f (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Visando à contribuição ao estudo da oncologia e ao aumento da sobrevida de cadelas com neoplasias mamárias, o objetivo deste trabalho é investigar as possíveis alterações no gene supressor de tumor p53 em tumores de mama relacionando-as com a idade, raça, tipo histológico, número e localização da neoplasia, tempo de progressão e característica macroscópica do tumor (tamanho e presença de úlcera). Foram avaliadas 30 amostras de neoplasias de mama de cadelas. Este material foi distribuído em cinco grupos, de acordo com a classificação histopatológica do tumor (adenoma, tumor misto benigno, carcinoma, tumor misto maligno e sarcoma). O Grupo Controle compreendeu cinco amostras de tecido mamário sem alterações patológicas. As possíveis mutações existentes nos exons 5 a 8 do gene p53 foi analisado por meio da técnica de PCR (reação em cadeia de polimerase) e seqüenciamento. Foram observadas cinco mutações em neoplasias malignas e uma mutação em neoplasia benigna. Dessas mutações, todas eram “missense” e três delas eram do tipo “frameshift”. Comparando as mutações com os outros parâmetros clínicos, concluiu-se que alterações em p53 devem ser originadas em um estágio inicial na carcinogênese mamária canina e mutações em p53 podem estar relacionadas com a malignidade do tumor. / Aiming at the contribution for oncology’s study and the improvement of survival time of bitches with mammary neoplasm, the purpose of this research is investigate the possible alterations in tumor suppressor gene p53 in mammary neoplasm, relating then with age, breed, tumor histologic classification, neoplasm location (which mammary gland and if they are unique or multiples), progression time and neoplasm macroscopic characteristic (size and ulceration presence). Thirty canine mammary neoplasm patterns were evaluated. This material was distributed in five groups according to histologic classification of the tumor (adenoma, benign mixed tumor, carcinoma, malignant mixed tumor and sarcoma). The Control Group included five mammary tissue patterns with no pathological alterations. Possible mutations at 5 to 8 exons within p53 gene was analyzed by the PCR technique (“Polymerase Chain Reaction”) and sequencing. Five mutations were observed in malignant mammary neoplasm and one mutation in benign neoplasm. About these mutations, all were missense and three of them were frameshift. Comparing the mutations with the other clinical parameters, it was concluded that p53 alterations must be started in an initial stage in canine mammary carcinogenesis and p53 mutations can be related with the tumor malignancy.

Made available in DSpace on 2016-09-27T13:39:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-10-29. Added 1 bitstream(s) on 2016-09-27T13:45:07Z : No. of bitstreams: 1 000870610.pdf: 912715 bytes, checksum: fd85044be54e4bd25cdd15279986ef8a (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Until now, the disease caused by the canine coronavirus (CCoV) was not fully elucidated, the CCoV role in the pathogenesis of the illness is not quite established. Only after the SARS (Severe Acute Respiratory Syndrome) appearance in human beings caused by a coronavirus in 2002, isolated of a wild mammal, many studies were developed with coronavirus of different species in search of circulating variants. Few studies had reported the presence of CCoV and his variability's in Brazil. Viral enteritis are responsible by high morbidity taxes and mortality in the small animals clinic and, although there are several etiologic agents described, the CCoV and the CPV are not yet considered a major cause of acute gastroenteritis pathogens in young dogs. For the present study, were collected 100 stool samples from dogs attended in a veterinary hospital routine with clinical signs of gastroenteritis (hemorrhagic or not). The CCoV and CPV RNA were amplified in the tested samples. Some of the by-products from the CPV amplification were purified, sequenced, aligned and subjected to the GenBank for the detection of CPV-2c and characterization of CCoV. The CCoV was present in 6% of the analyzed samples, while CPV-2a was identified in 43% of samples and CPV-2b in 18% of the total. A sample pool submitted to the sequencing and filogenetic analysis identified the CPV-2c, reveling that the new variant is already present in Araçatuba - SP. The phylogenetic analyses for CCoV revealed that the studied samples belong to the Subtype CCoV-IIa (pantropic), strengthening the agent identification importance in the canine population, once that the actual available vaccines don't have the new variants so that the dogs from Brazil are susceptible to severe outbreaks, as described in other countries. / FAPESP: 2013/249514

Made available in DSpace on 2016-09-27T13:39:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-01. Added 1 bitstream(s) on 2016-09-27T13:45:08Z : No. of bitstreams: 1 000870641.pdf: 755047 bytes, checksum: 904fea33f34db16ae9b0d792a4458f19 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / The present study investigated the occurrence of T. foetus in cats from Brazil. T. foetus is a protozoan that in cats it colonizes the colon resulting in colitis. The major clinical sign is chronic large bowel diarrhea. Infected cats may have spontaneous resolution of diarrhea, but they can remain infected for all life. In veterinary medical practice, there are three most widespread diagnostic methods that identify T. foetus in animal feces: direct examination of feces under the microscope, culture and PCR. In the present study, it was compared two diagnosis methods, direct examination of feces and PCR. And also it was done the genetic sequencing. Fecal samples from 129 cats were collected by rectal flush technique. Each sample was examined by optical microscopy (direct examination). The presence of T. foetus DNA was verified using PCR by amplification of 347 bases pairs from the primers TFR3 and TFR4. Amplicons of positive cases were sequenced. T. foetus was observed in one sample by direct microscopic examination of feces while PCR was positive in five cats (6.45%). Statistical analyses showed no significant associations between T. foetus infection and sex, age, breed, presence of diarrhea, history of diarrhea, previous treatment, lifestyle, environment, origin of the animals, and co-infections. The isolate of T. foetus showed 100% identical sequences with other T. foetus isolates from cats around the world and revealed a single nucleotide polymorphism (T>C) when compared with T. foetus isolate from cattle. Due the increasing interest in this parasite in cats, the present study contributes in further reporting the worldwide occurrence of T. foetus in cats because it is the first study about its occurrence in Brazil. / FAPESP: 2011/112436

Made available in DSpace on 2016-09-27T13:40:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-30. Added 1 bitstream(s) on 2016-09-27T13:45:09Z : No. of bitstreams: 1 000870502.pdf: 1430602 bytes, checksum: eba1658f28dab4763efaa8eafcf24462 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / The eimeriosis is an important parasitic disease in rabbits, that can host 11 species of Eimeria. Cryptosporidiosis is a zoonotic disease that can be transmitted through food, drinking water and by contact with infected animals and people. The objective of this study was to verify the occurrence of Eimeria spp. and Cryptosporidium spp. in fecal samples of rabbits, perform their molecular classification and relate the presence of the parasites to the different categories in the Brazilian farms. Fecal samples (n = 514) were collected from 21 farms. The oocysts were purified and visualized by microscopy. Fifty five samples positive for Eimeria spp. using microscopy were subjected to polymerase chain reaction (PCR) for amplification of a partial fragment of the ITS1 region of the rRNA gene of Eimeria spp. and the 18S rRNA and the gp60 glycoprotein genes of Cryptosporidium spp.. The microscopy revealed positivity of 19.45% (100/514) for Eimeria spp. and 1.56% (8/514) for Cryptosporidium spp.. The PCR identified E. exigua (14.5%), E. flavescens (61.8%), E. intestinalis (16.36%), E. irresidua (16.4%), E. magna (50.9 %), E. media (3.6%), E. perforans (36.4%), E. piriformis (20.0%), E. stiedai (7.3%) and E. vejdovskyi (7.3 %). Higher positivity was observed in mini rabbits 33.17% (69/208), young rabbits 46.67% (35/75) and in lactating females 24.47% (23/94). Seven samples were positive by PCR (12.73%; 7/55) for Cryptosporidium spp.. Molecular analysis revealed Cryptosporidium cuniculus (18S rRNA) and C. cuniculus subtype VbA21 (gp60) in young rabbits and in does