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I det här projektet extraherades värdefulla komponenter från äpple-pomace som är en restprodukt från cidertillverkning. Restprodukten var tillhandahållen av en svensk cidertillverkare. Extraktionen fokuserades mot pektin och fenolföreningar som uppvisar antioxiderande aktivitet. Extraktionsprocessen designades ur ett miljövänligt perspektiv, inga skadliga kemikalier användes. Fenolära substanser extraherades initialt från pomacen med en vattenlösning innehållande 50% etanol. Därefter studerades kompositionen och den antioxiderande aktiviteten i dessa extrakt. Fokuseringen i projektet låg främst på pektin. Pektin extraherades med subkritiskt vatten vid tre olika pH (3,5 och 7) samt vid två skilda temperaturer (120°C och 140°C) i antingen 5, 10 och 15 minuters sekvenser. Pektinet karakteriserades med avseende på extraktionsutvinningsgrad, sockerkomposition, molekylvikt och antioxiderande aktivitet. Resultaten jämfördes för att undersöka effekten av pH, temperatur och extraktionstid. Gelningsegenskapen hos olika pektin studerades som ett bevis på koncept i ett empiriskt experiment där hög-viskösa vätskor bestående av 5% pektin och 60% sackaros framställdes. Extraherade fenolföreningar har potential att fungera som naturligt utvunna antioxidanter i kosmetiska produkter och pektin kan tänkas användas som reologimodifierare i vattenbaserade formuleringar med låga pH-värden utan behov av kemiska modifieringar. / In this project, apple pomace from a Swedish cider making factory as a by-product was used as a raw material to extract valuable compounds. The extraction was focused on pectin and phenolic compounds with antioxidant activity. For the extraction procedures environmentally friendly processes were chosen without using any harsh chemicals. Phenolic compounds wereinitially extracted from the pomace using 50% aqueous ethanol and then the composition, total phenolic content and antioxidant activity were studied in these extracts. The main focus was on pectins, which were extracted by subcritical water at three different pH conditions (pH 3, 5 and 7) and two different temperatures (120°C and 140°C) in 5-, 10- and 15 minute sequences. Then the pectins were characterized in terms of extraction yield, sugar composition, molecular weight and antioxidant activity and the results were compared in terms of the effect of pH, temperatur eand extraction time. The gelling properties of the different pectins were also studied as a proof of concept in an empirical experiment, where highly viscous liquids were obtained at 5% pectinand 60% sucrose content. In summary, the extracted phenolic compounds have potential to function as naturally derived antioxidants in cosmetics and the pectin may be used as a rheology modifier in water-based formulations of low pH without any additional chemical modifications.

<i>Cryptosporidium parvum</i> is a protozoan parasite historically associated with waterborne and more recently foodborne outbreaks of diarrheal illness. Contamination of certain foods, such as unpasteurized apple cider, with infective oocysts may occur as oocysts are shed in the feces of common ruminants like cattle and deer that graze in and around orchards. Cryptosporidiosis can result in a severe illness for previously healthy individuals and a life-threatening illness in immunocompromised individuals. Disease occurs after the ingestion of small infective oocysts (4 to 5 mm in size). The relatively thick membrane of the oocysts allows them to be resistant to chlorine and many other environmental pressures, making oocysts difficult to inactivate. In this study, alternative treatments to pasteurization were evaluated for their ability to inhibit <i>C. parvum</i> oocyst viability in fruit juices. Oocyst viability was analyzed with a cell culture infectivity assay, using a human illeocecal cell line (HCT-8) that is most similar to human infection. The percent inhibition of infection by each treatment was determined along with the corresponding log reduction for the treatments found to be most effective. Infection by treated oocysts was compared to that of control untreated oocysts. Cell monolayers were infected with 10⁶ treated oocysts or a series of 10-fold dilutions. Parasitic life stages were visualized using an immunohistochemistry system and 100 microscope fields counted per monolayer. Organic acids and H₂O₂ were added on a wt/vol basis to apple cider, orange juice, and grape juices. Malic, citric, and tartaric acids at concentrations from 1%-5% inhibited <i>C. parvum</i> infectivity of HCT-8 cells by up to 88%. Concentrations ranging from 0.025%-3% H₂O₂ were evaluated where addition of 0.025% H₂O₂ to each juice resulted in a >5 log reduction of C. parvum infectivity as determined with an MPN-based cell culture infectivity assay. Treating apple cider, orange juice, and grape juice with ozone for a time period of 30 seconds up to 15 minutes at 6° and 22°C (0.9 g/L flow rate) inhibited C. parvum viability to > 90% as monitored in the cell culture assay. It is hypothesized that oocyst wall proteins that are necessary for infection are oxidized by the reactive oxygen species generated from the decomposition of the ozone and hydrogen peroxide treatments. These treatments or combinations thereof may offer potential alternatives to traditional pasteurization for fruit juices to successfully inhibit <i>C. parvum</i> viability. / Ph. D.

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is a polyphagous, invasive, and highly significant pest of mid-Atlantic fruit orchards. Its capacity to invade orchards season-long is believed to be facilitated by the availability of wild host plants near orchards and the high dispersal capacity of adults and nymphs. Interactions between H. halys and its hosts were investigated via laboratory and field experiments. Rearing H. halys nymphs in the laboratory on several of its known wild and tree fruit hosts used alone and in combination showed that peach was much more suitable for nymphal development and survival than was apple, tree of heaven, or catalpa and that nymphal performance was enhanced on mixed host diets. Nymphs reared on different host plant diets yielded adults that differed in protein, sugar or lipid content, offering new insights into plant diet effects on H. halys nutrition. The majority of tree species identified from unmanaged woodlands next to apple orchards in Virginia and West Virginia were known hosts of H. halys. Passive trunk traps were developed to capture H. halys nymphs walking up and down tree trunks and used to measure their temporal patterns of movement onto and off of wild and tree fruit hosts at the orchard-woodland interface via weekly assessments during two consecutive seasons. More upward- than downward-walking nymphs were captured, which may be partially explained by the positively phototactic and negatively geotactic behavior of H. halys nymphs demonstrated in laboratory studies. Most nymphs captured while walking up and down trunks were second instars, although the data also reflected temporal changes in the distribution of instars and temporal variation in the numbers of nymphs captured. A field study to characterize injuries to apple and peach fruit at harvest from feeding by young or older nymphs early or late in the season revealed that feeding by young nymphs early in the season resulted in least injury to both crops. In combination, these studies greatly improve our understanding of the biology and ecology of H. halys in eastern fruit orchards and provide relevant data that will inform the development of ecologically-based management programs for it. / Ph. D.

2001年，消聲已久的蘋果公司推出iPod，改寫了音樂產業的風貌，造成全球搶購的熱潮，自此之後，蘋果公司陸續推出許多產品，在全世界屢獲好評，在台灣亦如是。然而，台灣目前沒有Apple Store，因此欲於實體店面購買蘋果產品的消費者，會至蘋果在台的各大經銷商購買，而目前在台灣有四大優質經銷商，分別是Youth、德誼、Studio A和i Store，此四家的業績近乎佔盡台灣蘋果銷售市場。因此，本研究以蘋果公司最重要的兩項元素「服務創新」及「體驗行銷」作為本研究內涵，以此檢視台灣蘋果四大優質經銷商在「顧客滿意度」與「顧客忠誠度」上是否獲得消費者的認同。 　　本研究以調查法為主，於2011年4月9日至2011年5月7日間透過網路平台發放問卷，總計填答者471位，剔除無效問卷31份，有效問卷共440份，有效回收率為93.4 %，並以深度訪談為輔，訪問台灣蘋果四大優質經銷商於台北旗艦店之店經理與副店經理。 　　透過量化與質化的研究結果可得以下四大結論，第一，服務場景與流程創新是讓消費者辨別經銷商之關鍵；其二，消費者感受到的五感體驗將烙印於消費者心中；其三，顧客滿意度由服務創新與體驗行銷共同決定；最後，顧客忠誠度的表現是為企業獲利指標。另外，透過本研究長期觀察與實證結果，提出下列幾點建議予台灣蘋果公司參考：爭取優先上市權、舉辦活動並增加露出、播送電視廣告、提高服務品質、遵守蘋果總公司服務條款、建立專門蒐集消費者意見之管道。 / In 2001, the Apple Inc. which had been quiet for a long time announced their product – iPod, and soon rewrote the style of global music industry. The representing product – iPod also raised up shopping rush all over the world. Henceforward, Apple Inc. brought lots of products and became popular around the world, so did Taiwan. Even though, Apple Inc. didn’t institute any Apple Stores in Taiwan, the only way to buy Apple’s products personally is to visit dealers which acquire authorization. In addition, there are four APRs (Apple Premium Reseller) in Taiwan, include Youth, Dataexpress, Studio A, and istore, they also hold overwhelming majority of Apple products selling in Taiwan. As a result, this research is based on service innovation and experiential marketing that two factors the Apple Inc. emphasize most, and examine whether those four APRs had obtain identification from consumers in customer satisfaction degree and customer loyalty or not. 　This research used survey procedure to gather feedbacks from consumers during 9th April to 7th May in 2011 by uploading internet survey platform. There were 471 answer sheets on the platform above, 31 of them were invalid and 440 were valid, achieve the scale at 93.4%. We also used depth interview and had interviews with shop managers and vice-shop managers of those four APRs. 　Combining the two dimensions, this research had four main conclusions. First, Services cape and Procedures innovations are key points for consumers to recognize resellers. Second, five source of sense experience that consumers truly experience will make deep impressions in their mind. Third, customer satisfaction degree depends on service innovation and experiential marketing. The last but not least, the certain appearance of customer loyalty is as the index of company’s profit. According to long term observation and the conclusions of empirical research, we recommend few suggestions for the branch in Taiwan of Apple Inc. as follow. First, try to fight for the position of debut product, try to hold more selling activities and increase exposure, try to broadcast more TV commercials, try to raise service quality, try to obey service principles and guidelines established by Apple Inc., and try to set up a special channel to collect opinions from consumers.

<p>This study aims to compare two ethnographies of organizational culture – one from the discipline of Social Anthropology and one from the discipline of Organizational Studies – considering their purpose, their method, and their concept of culture. Despite big similarities, or perhaps thanks to the similarities, some fundamental differences are made visible. The discipline of Organizational Studies has, compared to that of Social Anthropology, regarding the research method being used, less focus on, and less participation in, the informal social life of the organizational culture of the study. Regarding the concept of culture the Organizational Studies consider culture containing more of unity, stability and clear boundaries, compared to Social Anthropology. Further – the Organizational Studies has more focus on power and structure, while Social Anthropology has more focus on meaning and interpretation. Finally – the research purpose of studies in Organizational Studies is a bit more normative in its characteristic, than that of Social Anthropology. The study is concluded with a discussion of the future of Social Anthropology and its studies of organizational culture. An argumentation is conducted intended to get the two disciplines to learn from each other in the area of organizational culture, and also intended to show the discipline of Social Anthropology a road to relevant studies in the area of organizational culture.</p> / <p> </p><p>Denna studie syftar till att jämföra två etnografier inom organisationskultur - en från disciplinen socialantropologi och en från disciplinen organisationsteori - med fokus på deras respektive syfte, metod och kulturbegrepp. Trots stora likheter, eller kanske tack vare likheterna, syns några grundläggande skillnader. Organisationsteorin har, jämfört med socialantropologin, angående forskningsmetod, mindre fokus på och mindre deltagande i det informella sociala livet i den studerade organisationskulturen. Angående kulturbegreppet så ser organisationsteorin kultur som något enhetligt, stabilt och tydligt avgränsat i större utsträckning än socialantropologin. Vidare har organisationsteorin större fokus på makt och struktur, medan socialantropologin har mer fokus på mening och tolkning. Slutligen är organisationsteorins syfte något mer normativ i sin karaktär än hos socialantropologin. Studien avslutas med en diskussion om socialantropologins framtid på området organisationskultur. En argumentation drivs i syfte att de två disciplinerna ska kunna lära från varandra och i syfte att visa en väg för socialantropologin att bedriva relevanta studier på området organisationskultur.</p>

This study aims to compare two ethnographies of organizational culture – one from the discipline of Social Anthropology and one from the discipline of Organizational Studies – considering their purpose, their method, and their concept of culture. Despite big similarities, or perhaps thanks to the similarities, some fundamental differences are made visible. The discipline of Organizational Studies has, compared to that of Social Anthropology, regarding the research method being used, less focus on, and less participation in, the informal social life of the organizational culture of the study. Regarding the concept of culture the Organizational Studies consider culture containing more of unity, stability and clear boundaries, compared to Social Anthropology. Further – the Organizational Studies has more focus on power and structure, while Social Anthropology has more focus on meaning and interpretation. Finally – the research purpose of studies in Organizational Studies is a bit more normative in its characteristic, than that of Social Anthropology. The study is concluded with a discussion of the future of Social Anthropology and its studies of organizational culture. An argumentation is conducted intended to get the two disciplines to learn from each other in the area of organizational culture, and also intended to show the discipline of Social Anthropology a road to relevant studies in the area of organizational culture. / Denna studie syftar till att jämföra två etnografier inom organisationskultur - en från disciplinen socialantropologi och en från disciplinen organisationsteori - med fokus på deras respektive syfte, metod och kulturbegrepp. Trots stora likheter, eller kanske tack vare likheterna, syns några grundläggande skillnader. Organisationsteorin har, jämfört med socialantropologin, angående forskningsmetod, mindre fokus på och mindre deltagande i det informella sociala livet i den studerade organisationskulturen. Angående kulturbegreppet så ser organisationsteorin kultur som något enhetligt, stabilt och tydligt avgränsat i större utsträckning än socialantropologin. Vidare har organisationsteorin större fokus på makt och struktur, medan socialantropologin har mer fokus på mening och tolkning. Slutligen är organisationsteorins syfte något mer normativ i sin karaktär än hos socialantropologin. Studien avslutas med en diskussion om socialantropologins framtid på området organisationskultur. En argumentation drivs i syfte att de två disciplinerna ska kunna lära från varandra och i syfte att visa en väg för socialantropologin att bedriva relevanta studier på området organisationskultur.

Sverige anses vara en av världens mest avancerade IT-nationer, där användningen av IT ökar inom både den offentliga och privata sektorn. Företag inom IT branschen expanderar i en starkt föränderlig bransch. En rad olika faktorer har varit grunden till Sveriges position som IT-nation, bland annat en ingenjörs- och innovationstradition över en lång tid, även faktorer som den utbreda användningen av IT i samhället och en snabbhet att implementera nya innovationer. IT-branschen i Sverige utgör 4 % av de sysselsatta i Sverige där omsättning, såväl som antalet anställda, faktiskt ökat även under konjunktursvackor. Syftet med studien är att undersöka om svenska nyhetsmedier i större utsträckning publicerat nyheter om Apple än om deras konkurrenter på smartphone marknaden. For att lättare kunna genomföra studien ska dessa frågeställningar besvaras: Hur har Apple lyckats bygga upp en hajp kring sitt varumärke i Sverige? Har Apple exponerats mer i svenska nyhetsmedier än konkurrenterna på smartphone marknaden? På vilket sätt påverkar svensk nyhetsmedia bilden av Apple? De teorier som används i studien är innovationsteorin, dagordningsteorin samt konsumentbeteendeteorin. Inledningsvis sker en fallstudie där det via sökmotorn på DN och SvD nätupplagor har summerats ihop antalet ordträffar på utvalda ord. Baserat på resultaten av ordanalys utvecklas en semistrukturerad telefonintervju som genomförs med DN. Parallellt sker en semistrukturerad intervju med en anställd på en reklambyrå inriktat mot hur specifikt Apple har gått tillväga för att skapa hajp. Utifrån vår undersökning och analys kom vi fram till att svenska nyhetsmedier inte styrs av bakomliggande faktorer när de offentliggör nyheter om företag. Publiceringar sker baserat på deras uppfattning om hur det allmänintresset ser ut hos läsarna. Apple är väldigt selektiva med att släppa ut information gällande företaget och runt deras produkter, detta resulterar i ett intresse från pressen att först publicera information från Apple, motiverat utifrån ett allmänintresse i samhället.

Les composés phénoliques sont impliqués dans les propriétés organoleptiques majeures des aliments dérivant d’une matrice végétale. Ils sont notamment responsables de l’amertume, de l’astringence et de la couleur des produits cidricoles. Le brunissement observé lors de la transformation des fruits est lié à la formation de pigments jaunes-orangés. Il est initié par l’activité d’une enzyme plastidiale, la polyphénol oxydase, qui catalyse l’oxydation des polyphénols en présence d’oxygène. Cela aboutit à la formation de quinones, espèces chimiques très réactives qui sont rapidement impliquées dans des voies réactionnelles ultérieures. Certains produits néoformés sont colorés. Un profilage des molécules colorées présentes dans quatre jus de pomme monovariétaux aux couleurs contrastées a été réalisé en CLHP-DBD-SM. Il a mis en évidence l’existence de tannins oxydés colorés et de composés jaunes-orangés non tannants. Parmi ces derniers, des produits d’oxydation de la phloridzine, dérivant de la classe des dihydrochalcones, ont été clairement identifiés (POPj, XGPOPj). Des approches couplées en milieu réel et solution modèle ont en outre permis d’identifier une nouvelle structure colorée dérivant d’un couplage oxydatif entre une dihydrochalcone et un flavanol monomère. Enfin, des déhydrotrimères d’épicatéchine ont pu être caractérisés en solution synthétique mais n’ont pas été détectés directement dans les jus. Par ailleurs, la couleur exprimée par les pigments formés peut subir des modulations en fonction de l’environnement physico-chimique. A titre d’exemple, la fermentation cidricole génère classiquement une décoloration des moûts ; or la composition du milieu fermentaire évolue sous l’influence de différents paramètres tels que le pH ou le potentiel redox. L’impact d’un milieu réducteur sur la couleur du POPj et d’une déhydrotriépicatéchine de type A, sélectionnés comme pigments modèles des jus de pomme, a été exploré par voltammétrie cyclique et spectroélectrochimie UV-visible. En outre, les changements de propriétés spectrales du POPj liés à son état de protonation ont été rationalisés par des approches de TD-DFT. / The phenolic compounds are involved in the main organoleptic properties of plant-derived foods. They are especially responsible for the bitterness, the astringency and the color of apple cider products. The browning observed during fruit processing is related to the formation of yellow-orange pigments. By its catalytic activities, the polyphenol oxidase, a plastidial enzyme, initiates the oxidation of polyphenols in the presence of oxygen. It leads to the formation of quinones, very reactive species which are quickly involved in further chemical reactions. Some of the neoformed products are colored. Four apple juices, showing contrasted colors, were analyzed by reversed-phase HPLC-DAD-MS in order to obtain their profiles in colored compounds. The presence of yellow-orange oxidized tanning and non-tanning compounds was highlighted. Within the latter compounds, some phloridzin oxidation products, deriving from dihydrochalcones, were clearly identified (POPj, XGPOPj). In parallel, coupled approaches in real media and model solutions led to the identification of a new colored structure deriving from an oxidative coupling between a dihydrochalcone and a flavanol monomer. Finally, catechin trimers were also characterized in synthetic medium but they were not detected directly in apple juices. Moreover, the color expressed by the yellow-orange pigments can suffer some variations as a function of the physical-chemical environment. For instance, the cider fermentation results generally in a loss of color in apple juices; but the composition of the fermentative medium evolves under the influence of several parameters, such as pH and redox potential. The impact of reducing conditions on the color of POPj and A-type dehydrotriepicatechin, both selected as model pigments of apple juices, was explored by cyclic voltammetry and UV-visible spectroelectrochemistry. Besides, the changes in spectral properties of POPj, related to its protonation state, were rationalized by TD-DFT calculations.

The main objective of this work is to analyze current trends in contactless payments and consequently implement it on the Czech market. On this base this thesis deals with several wearable devices. Furthermore on this base is also working in the application part, where is one of the secondary objectives of the work to determine consumer preferences on the market of the Czech Republic. One of the secondary objectives is create a brief summary of the history of payment cards and within the sociological aspect to find a certain analogy in introducing innovations in the field of payments towards current trends in contactless methods. Within the secondary objectives there are simultaneously defined key assumptions forms of payment for success on the market. The following section focuses on electronic payments. Specifically, it deals with trends in electronic payment services, where for its implementation aren´t required the physical presence of the card or chip. Furthermore there are analyzed alternative paths in the current payment methods, namely peer-to-peer platforms. On these platforms is based shared economy, which also represents an interesting potential for the possible development of payment field. Chapter with alternative methods for cashless is finished by analyzing digital currencies. The hypothesis of this thesis is whether Apple can have an impact on the area of contactless payments and how it can affect the entire sector. Part of the hypothesis is the claim that Apple manages to change the area of contactless payments, such as happenede in the music industry with iTunes and then in the telecommunications industry with a revolutionary iPhone. Potential benefit of Apple is defined by the method of description. Potential contribution of Apple Pay is characterized by logical deduction base on previous relationships. Conclusion is by quantitative research method, namely through the consumer research, assessed consumer preferences in the Czech Repulic and is also evaluated the hypothesis of potential benefits of Apple contactless payments.