Det moderna arbetslivet genomgår snabba och stora förändringar och ändrar  örutsättningarna för många yrkesgrupper i hur arbetet ska bedrivas på bästa och mest effektiva sätt. Detta får konsekvenser för olika yrkesgruppers arbetssituationer. En yrkesgrupp som inte har fått ett allt för nämnvärt fokus i forskningen kring förändrade arbetssituationer är HR-medarbetare. Syftet med den här studien är att öka förståelsen för hur HR-medarbetare upplever och hanterar sin arbetssituation utifrån arbetskrav och arbetsresurser. Tre frågeställningar har utformats för att omfamna och konkretisera syftet ytterligare. Studien baseras på fem utvalda respondenter, som arbetar som HR-generalister eller HR Business Partners. Studien har genomförts med en kvalitativ forskningsmetod i form av intervjuer och har utgått från en abduktiv ansats. Studien påvisar att det finns olika uppfattningar mellan HR-medarbetare om vilka resurser som anses viktiga i arbetet. Studien har även visat på att HR-medarbetare ställs inför höga arbetskrav i form av framför allt hög arbetsbelastning och stora rollkonflikter. Resultatet  visar att HR-medarbetarna vanligtvis hanterar svåra arbetssituationer genom problemfokuserade hanteringsstrategier. / The modern working life is going through big and rapid changes, which change the prerequisites for a lot of professions in how work should be conducted and utilized in best manners. This leads to consequences in the work situation for different professions. One profession that hasn’t a cquired a lot of focus in the research about changing work situations, is the HR Practitioners. The aim of this study is to increase the understanding in how HR Practitioners experience and manage their work situation from the basis of work demands and work resources. Three research questions have further been formed, with the aim to embrace and concrete the purpose of the study. The study is based on five selected candidates, who works as HR Generalists or HR Business Partners. The study has been conducted with a qualitative research method in the form of interviews and an abductive approach. The study demonstrates different opinions among the HR Practitioners about which kind of work resources that is considered most important at work. Results also show that HR Practitioners faces high work demands, mostly in the form of high work load and big role conflicts. Finally, the results show that HR Practitioners mostly apprehend and manage the severe work situations they face with a problem focused coping strategy.

Orientador: Mirian Cristina dos Santos / Resumo: O mexilhão dourado (Limnoperna fortunei) tem provocado transtornos ambientais e econômicos nas localidades onde foi introduzido. Dessa forma, o uso do seu resíduo moído como reparador da defasagem de nutrientes e de acidez em solos agrícolas pode vir a ser uma boa aplicação para esse molusco bioinvasor. Entretanto, apesar dessa ação reparadora, é necessária uma averiguação prévia da ausência de elementos potencialmente tóxicos no resíduo. Neste contexto, o presente trabalho foi realizado com o intuito de quantificar, em amostras do Limnoperna fortunei, os elementos chumbo, cádmio, alumínio, ferro e manganês, os quais dependo de suas quantidades podem ser tóxicos aos vegetais e aos demais seres vivos ao longo da cadeia alimentar. Para quantificação desses analitos foi utilizada a Espectrometria de absorção atômica com fonte contínua e de alta resolução. Foram desenvolvidos e otimizados programas de aquecimento para a determinação dos elementos traço chumbo e cádmio, empregando amostragem direta de sólidos e atomização com forno de grafite. A determinação direta de chumbo só foi possível com a utilização da calibração com o material de referência certificado para tecido de mexilhão (Mussel Tissue – 2976), empregando 5 µL da mistura 0,1% (m/v) Pd (NO3)2 + 0,05% (m/v) Mg (NO3)2 em presença de 0,05% (m/v) de Triton X-100 como modificador químico. As temperaturas de pirólise e atomização foram 1000°C e 2200°C, respectivamente. O estudo de massa permitiu a utilização de massas de am... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The golden mussel (Limnoperna fortunei) has caused environmental and economic disruption in the locations where it was introduced. Thus, the use of their ground waste as repairer of the gap of nutrients and acidity in soils may prove to be a good application for this mollusk bio invader. However, despite of this remedial action, a prior test of the absence of potentially toxic elements in the residue is required. In this context, the present study was performed in order to quantify, in samples of the Limnoperna fortunei, the elements lead, cadmium, aluminum, iron and manganese, which depending on their quantities they can be toxic to plants and other living beings throughout the food chain. To quantify these analytes was used high-resolution continuum source atomic absorption spectrometry. Heating programs were developed and optimized for the determination of lead and cadmium trace elements, using direct solid and graphite furnace atomization. The direct determination of lead was only possible with the use of calibration with certified reference material for mussel tissue (Mussel Tissue - 2976), using 5 uL of the mixture 0.1% (m/v) Pd (NO3)2 + 0.05% (w/v) Mg (NO3)2 in the presence of 0.05% (w/v) Triton X-100 as a chemical modifier. The temperatures of pyrolysis and atomization were 1000 ° C and 2200 ° C respectively. The mass study allowed the use of samples of mass within the range of 0.150 to 0.850 mg. For cadmium it was only performed calibration with standard 12.5 g/L sol... (Complete abstract click electronic access below) / Mestre

Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2015-11-30T13:23:21Z No. of bitstreams: 2 Dissertação - Flávia Carneiro Gonçalves - 2015.pdf: 3718226 bytes, checksum: 3024f921d7aec7030599eee358d0593f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-01T06:45:31Z (GMT) No. of bitstreams: 2 Dissertação - Flávia Carneiro Gonçalves - 2015.pdf: 3718226 bytes, checksum: 3024f921d7aec7030599eee358d0593f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2015-12-01T06:45:31Z (GMT). No. of bitstreams: 2 Dissertação - Flávia Carneiro Gonçalves - 2015.pdf: 3718226 bytes, checksum: 3024f921d7aec7030599eee358d0593f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-07-30 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This study objectives to evaluate the effect of an inappropriate night cooling condition, it is practiced by some shops with processed mozzarella cheese and conventional mozzarella chemical profiles, using nuclear magnetic resonance technique (NMR). The simulation of inappropriate cooling was performed using a Styrofoam box with ice, not covered, and leaving the system to reach thermal equilibrium with the ambient temperature during the night. To make studies with processed mozzarella cheese was acquired two packages of the same batch, at local shops. Subjecting the spectral data, coming from the analysis made with HR-MAS, in the chemometric treatment was observed a tendency of discrimination in the samples exposed to different cooling conditions, and signals concerning lactose and some of fatty acids had greater influence in the observed separation. For the mozzarella cheese, in addition to NMR studies, it was also performed physical and chemical characteristics studies. For these studies, it has been acquired twenty-two trays containing sliced cheese, in the local market, which were divided into two sets with eleven trays each. Samples were analyzed in nature, using HR-MAS probe, and the crude extract using TBI probe. The identification of metabolites was done from the HSQC experiments, J-resolved and NMR ¹H, the last one being also used in the relative quantification of twelve metabolites. For the mozzarella cheese maintained under improper cooling conditions, there was a reduction in the galactose content and an increasing of amino acids and organic acids contents. The physicochemical analysis of pH, saturated fat and protein showed no significant differences that could be correlated with the different conditions of sample cooling. Finally, it has been concluded that NMR technique and chemometric tools are promising in identify the cheese samples submitted to different cooling conditions that has been studied, which could not be done using the physical and chemical parameters commonly used in cheese quality analysis. / Neste estudo vislumbrou-se avaliar a o efeito da condição de refrigeração noturna inadequada, praticada por alguns estabelecimentos comerciais, no perfil químico dos queijos tipo processados sabor mozarela e tipo mozarela convencional, fazendo uso da técnica de Ressonância Magnética Nuclear (RMN). A simulação da refrigeração inadequada foi realizada utilizando-se uma caixa de isopor contendo gelo, sem tampa, deixando o sistema atingir o equilíbrio térmico com o ambiente no decorrer da noite. Para os estudos com o queijo tipo mozarela processado adquiriu-se dois pacotes, pertencentes a um mesmo lote, no comércio local. Submetendo-se os dados espectrais, advindos das análises fazendo uso da sonda HR-MAS, ao tratamento quimiométrico, observa-se uma tendência de discriminação das amostras submetidas a diferentes condições de refrigeração, sendo que os sinais referentes a lactose e de alguns ácidos graxos tiveram maior influencia na separação observada. Já para o queijo tipo mozarela convencional, além dos estudos de RMN, realizou-se também estudos de características físico-químicas. Para isso, foram adquiridas no comércio local vinte e duas bandejas contendo queijo fatiado, as quais foram divididas em dois conjuntos com onze bandejas cada. Foram realizadas análises da amostra in natura, fazendo uso da sonda HR-MAS, e do extrato bruto, fazendo uso da sonda TBI. A identificação de metabólitos foi feita a partir dos experimentos HSQC, J-resolved e RMN de ¹H, sendo este último utilizado também na quantificação relativa de doze metabólitos. Para o queijo tipo mozarela mantido sob condições inadequadas de refrigeração observou-se uma redução no teor de galactose e aumento no teor de aminoácidos e ácidos orgânicos. As análises físicoquímicas de pH, gordura total e proteína total não mostraram diferenças significativas que pudessem ser correlacionadas com as diferentes condições de refrigeração das amostras. Nesse sentido, conclui-se que a técnica de RMN e ferramentas quimiométricas mostraram-se promissoras na identificação das amostras de queijo submetidas às diferentes condições de refrigeração estudadas, o que não pôde ser feito utilizando-se os parâmetros físico-químicos rotineiramente empregados em análises da qualidade de queijos.

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-27T11:23:07Z No. of bitstreams: 2 Tese - Rodolpho de Campos Braga - 2015.pdf: 15048949 bytes, checksum: 8c5fe5ec8f286e33101963872c33d8d5 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-27T11:26:29Z (GMT) No. of bitstreams: 2 Tese - Rodolpho de Campos Braga - 2015.pdf: 15048949 bytes, checksum: 8c5fe5ec8f286e33101963872c33d8d5 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-09-27T11:26:29Z (GMT). No. of bitstreams: 2 Tese - Rodolpho de Campos Braga - 2015.pdf: 15048949 bytes, checksum: 8c5fe5ec8f286e33101963872c33d8d5 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-09-18 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / In view of the alarming scenario of visceral leishmaniasis in Brazil and in the world, especially due to the increasing number of cases of drug resistance and due to the few drugs available, it is essential to search for new therapeutic alternatives for this parasitosis. The complete sequencing of the genome of the main species of Leishmania opened great possibilities in understanding these diseases and initiated the post-genomic era of drug discovery against kinetoplastids. In this context, the enzyme 14 a-sterol demethylase (CYP51) of Leishmania is especially involved in the biosynthesis of ergosterol, the main sterol membrane and vital to the parasite. Furthermore, it was recently shown that inhibition of CYP51 of L. donovani is essential for the growth of the parasite, and therefore, is a validated target for the development of new leishmanicidal drugs. The aim of this work was the development and implementation of integrated strategies in medicinal chemistry to identify new bioactive compounds against L. infantum using CYP51 enzyme as molecular target. For this, we compiled, integrated and prepared the largest publicly available data sets related to CYP51 and phenotypic assays for Leishmania infantum amastigotes. Virtual screening models (VS) were constructed and extensively validated and applied to filter over 1 million of commercial compounds. The best models for VS were ROCS (LBDD) and pharmacophore (SBDD), with an area under the ROC curve (AUC) values of 0.86-0.90. The consensus between the two models had greater performance, with AUC of 0.93 and high recognition ability active ligands in the top 1% hits. Quantitative structure-activity relationship (QSAR) models robust and predictive were generated and validated for L. infantum (amastigote forms). The models were able to discriminate inactive active compounds with correct classification rate (CCR) values of 0.77 to 0.95 when evaluated for the external validation set. After the virtual screening, QSAR models were used to assist in the final selection of the compounds to be experimentally evaluated. This strategy allowed the identification of 12 compounds that were selected and acquired for in vitro assays against Leishmania (L.) infantum (MHOM / BR / 1972 / LD) in promastigote and amastigote forms, and determination of selectivity/cytotoxicity in NCTC in mammalian cells. Of the twelve compounds tested, six of them showed 50% inhibitory concentration (IC50) values raging from 3.48 to 58.94 μM and were more potent than the standard drug meglumine antimoniate, which is the drug of choice for the treatment of all forms of leishmaniasis. Three most promising compounds (LabMol-007, LabMol-009 and LabMol-012), had activity in leishmanicidal as amastigote and selectivity index promising (IC50 < 5.21 μM and selectivity index > 6.8) and were selected as new hits. We analyzed the parasite metabolic changes in the presence of known CYP51 inhibitors and new inhibitors using HR-MAS NMR 1H . We observed major changes in the energetic metabolism, amino acids catabolism, sterol biosynthesis, purine biosynthesis and thiol-redox system of the parasite. The three hit compounds identified in this work will continue in the drug development process, being necessary to carry out in vivo studies, elucidation of the mechanism of action, hit optimization, as well as the study of pharmacokinetic properties and toxicity. / Tendo em vista o quadro alarmante da leishmaniose visceral no Brasil e no mundo, especialmente devido ao crescente número de casos de resistência e aos poucos medi- camentos disponíveis, é imprescindível a busca de novas alternativas terapêuticas para esta parasitose. O sequenciamento completo do genoma das principais espécies de Leish- mania abriu grandes possibilidades no entendimento desses organismos e iniciou a era pós-genômica da descoberta de fármacos contra tripanossomatídeos. Nesse contexto, destaca-se a enzima 14 a-esterol desmetilase (CYP51) de Leishmania, que é especial- mente envolvida na biossíntese do ergosterol, principal esterol de membrana e vital para o parasito. Além disso, recentemente foi demonstrado que a inibição da CYP51 de L. donovani é essencial para o crescimento do parasito, sendo, portanto, um alvo validado para o desenvolvimento de novos fármacos leishmanicidas. O objetivo geral deste trabalho foi o desenvolvimento e a aplicação de estratégias integradas em Química Medicinal para a identificação de novas substâncias bioativas contra L. infantum, utilizando a enzima CYP51 como alvo molecular. Para isso, foram compilados, integrados e preparados os maiores conjuntos de dados disponíveis publicamente relacionados a CYP51 e a ensaios fenotípicos para Leishmania spp. Modelos de triagem virtual (VS) foram construídos, exaustivamente validados e aplicados para filtrar uma quimioteca com mais de 1 milhão de compostos comerciais. Os melhores modelos para a VS foram o ROCS (LBDD) e o farmacofórico (SBDD), com valores de área sob a curva ROC (AUC) de 0,86-0,90. O consenso entre esses dois modelos foi superior com valor de AUC de 0,93 e alta capacidade de reconhecimento de ligantes ativos no topo de 1% hits. Modelos de relação quantitativa entre estrutura e atividade (QSAR) robustos e preditivos foram gerados e validados para um conjunto de dados de compostos com atividade contra L. infantum (formas amastigota). Os modelos de QSAR foram capazes de discriminar compostos ati- vos de inativos com uma taxa de acerto (CCR) de 0,77-0,95, quando avaliados utilizando o conjunto de validação externa. Após a triagem virtual, os modelos de QSAR foram usados para auxiliar na seleção final dos compostos a avaliados experimentalmente. Essa estratégia permitiu a identificação de 12 compostos que foram selecionados e adquiridos para realização de ensaios de atividade biológica in vitro contra Leishmania (L.) infantum (MHOM/BR/1972/LD) em formas promastigotas e amastigotas, e determinação de sele- tividade/citotoxidade em células mamíferas NCTC. Dos doze compostos testados, seis deles apresentaram valores de concentração inibitória de 50% (IC50) entre 3,48-58,94 μM e foram mais potentes que o fármaco padrão antimoniato de meglumina, que é o fármaco de primeira escolha para o tratamento de todas as formas de leishmaniose. Três compostos mais (LabMol-007, LabMol-009 e LabMol-012) tiveram atividade em leishmanicida na forma amastigota e índice de seletividade bastante promissores (IC50 < 5,21 μM e índice de seletividade > 6,8) e foram selecionados como novos hits. Analisou-se as alterações metabólicas do parasito na presença inibidores conhecidos de CYP51 e dos novos inibi- dores, empregando-se RMN HR-MAS 1H. Foram observadas alterações principais nas vias de metabolismo energético, catabolismo de aminoácidos, biossíntese de esteróis, metabolismo purinas e no sistema tiol-redox do parasito. Os três hits identificados neste trabalho prosseguirão no processo de desenvolvimento de novos fármacos, sendo necessá- ria a realização de estudos in vivo, elucidação do mecanismo de ação, otimização dos hits, além do estudo de propriedades farmacocinéticas e toxicidade.

La principale limite en chirurgie hépatobiliaire est représentée par l’insuffisance hépatocellulaire (IHC) posthépatectomie (Hx) ou la dysfonction du greffon (EAD) après transplantation (TH). Peu d’études ont évalué le métabolisme du foie dans son ensemble, du fait du manque de technique utilisable en clinique. La métabolomique HR-MAS-RMN pourrait pallier à ce manque. Le but de cette thèse était d’évaluer l’apport de cette technique en chirurgie hépatobiliaire.En TH (n=42), le profil métabolique (PM) prédisait le risque d’EAD et identifiait le lactate et la phosphocholine comme biomarqueurs permettant d’envisager un matching métabolique. Après Hx majeure (n=45), le PM prédisait la survenue d’un décès par IHC. Ce PM différait du profil cirrhotique en décompensation et était compatible avec celui de système cellulaire prolifératif. Une étude préliminaire montrait que le PM prédisait la récidive à 1 an après hépatectomie. Ce travail montre l’intérêt de la métabolomique par HR MAS RMN pour prédire l’issue d’une Hx ou d’une TH dans un temps compatible avec la clinique. Ces données orientent vers la piste de l’intervention métabolique en chirurgie hépatique. / One of the main limits in liver surgery is the risk of liver failure (LF) after hepatectomy (Hx) or graft dysfunction (EAD) after liver transplantation (LT). Few studies have evaluated global liver metabolism, probably due to the lack of clinically relevant techniques. HR-MAS-NMR metabolomics may fulfill this lack and the goal of this work was to evaluate its capacity to predict early outcomes after hepatectomy and LT. In LT (n=42), metabolic profile predicted EAD and lactate and phosphocholine were potent biomarkers providing means for metabolic matching. In liver biopsies harvested at the end of major Hx (n=45), metabolic profile predicted PHLF. The profile at risk of LF differed from that of decompensated cirrhosis but correlated to that of proliferative multicellular systems. A preliminary study showed that the metabolic profile predicted the risk of liver metastases recurrence at 1 year. This work underlines the potential value of HR-MAS-NMR metabolomics in the prediction of short-term outcomes in liver surgery. It provides clues to be further investigated for future evaluation of metabolic intervention in the field of liver surgery.

Cette thèse présente une approche multicompartimentée en métabolomique appliquée au diagnostic des tumeurs indéterminées de la thyroïde et à la caractérisation de l’effet du fructo-oligosaccharide sous condition de régime hyperlipidique chez la souris. L’objectif était de montrer qu’une telle approche permettrait de visualiser de façon plus globale des modulations du métabolisme induites, et par conséquent, d’améliorer la pertinence des marqueurs discriminants identifiés. Dans le cas de l'analyse nutritionnelle, l’analyse mono-compartimentée des différentes organes/segments intestinaux des souris ont permis de mettre en évidence les voies métaboliques affectées par le régime hyperlipidique mais pas d’observer un effet significatif du prébiotique FOS. Dans le cas du cancer de la thyroïde, l’étude multicompartimentée n’a pas pu être mise en place dans de bonnes conditions (problème de prélèvement d’échantillons). Cependant, l’analyse métabolomique monocompartimentée basée sur l’analyse HR-MAS des ponctions prélevées à l’aiguille fine a mené à une discrimination significative entre les lésions bénignes et malignes avec une prédictivité similaire aux tests moléculaires actuellement disponibles. En parallèle, nous avons exploré la technique HR-MAS à rotation lente dans le but de préserver l’intégrité des tissus au cours des expériences. L’utilisation de cette technique s’accompagne d’un certain nombre d’inconvénients que nous avons contournés en utilisant des séquences RMN particulières, et en mettant en place un protocole robuste de préparation d’échantillons. Enfin, nous avons évalué le filtre T1ρ et son application en métabolomique comme alternative au filtre T2. / This thesis presents a multicompartmental metabolomics approach applied to the diagnosis of indeterminate thyroid tumours and to the characterization of the effect of fructo-oligosaccharide (FOS), a prebiotic, under high fat diet condition in a mouse model. The aim of this project is to show that such approach could lead to a more global visualization of the induced metabolic modulations, and therefore, improve the identified discriminant markers relevance. Regarding the diet study, the mono-compartmental analysis of the different mouse organs/intestine segments enabled us to identify metabolic pathways affected by the high fat diet whereas the effect of FOS could only be characterized for the feces samples collected at day 28. Regarding the thyroid cancer study, the multi-compartmental approach could not have been continued due to sample handling issues. However, the classical metabolomics analysis of the fine-needle aspiration biopsies (FNAB) from patients with benign or malignant tumours led to a clear discrimination between both groups with a predictivity similar to that of commercial diagnosis tests. In the meantime, we explored the slow-spinning NMR HR-MAS technique in order to preserve the integrity of the tissues during the experiments. The use of this technique is accompanied by a number of drawbacks that we have avoided using special NMR sequences, and putting in place a robust protocol for sample preparation. Finally, we evaluated the T1ρ filter and its applications to metabolomics as an alternative to T2 filter.

Ce travail a pour finalité d'analyser l'évolution de la GRH au sein des entreprises marocaines vers l'avènement d'une nouvelle tendance « Talents », en prenant le cas du secteur bancaire. Ce secteur connaît actuellement un contexte concurrentiel rude, notamment en présence d'une conjoncture économique difficile, une crise financière internationale, et un turn-over important. Nous avons choisi une méthodologie qualitative qui fait appel à des échanges, des entretiens en face à face, et aussi téléphoniques, réalisés sur le terrain. Cette thèse vise à comprendre l'approche « Talents » non seulement du point de vue de l'entreprise, mais également de la discerner telle que nous pouvons l'apercevoir chez le salarié. En effet, l'évolution actuelle de la mondialisation peut être repérée dans plusieurs domaines, et principalement dans les marchés financiers. Cela entraine certes des changements dans le fonctionnement de ce secteur. D'où l'apparition des nouvelles pratiques RH. L'objectif principal de la présente recherche est de voir si la gestion des talents peut s'imposer au sein des entreprises marocaines comme une nouvelle pratique de la GRH. Pour ce faire, nous allons tout d'abord déterminer la politique actuelle mise en place par les banques en termes de GRH, puis nous allons essayer de discerner la place de la nouvelle tendance « Talents » au sein de celles-ci, et enfin, nous allons analyser les résultats obtenus, et confirmer ou infirmer les hypothèses formulées à la lumière de notre étude empirique. Les résultats de ce travail peuvent faire l'objet de recommandations pratiques aux banques marocaines, afin de revoir leur politique RH, et de faire face aux enjeux actuels. / The purpose of this work is to analyze the evolution of HRM in the Moroccan companies to the advent of a new trend "Talents", taking the case of the banking sector. This sector is currently experiencing a severe competitive environment, particularly in the presence of a difficult economic environment, international financial crisis, and high turnover. We chose a qualitative methodology that involves exchanges, face-to-face discussion or phone interview. This thesis aims to understand the "Talents" not only in terms of business approach, but also to discern it as we can perceive it from the employee. Indeed, the current evolution of globalization can be spotted in several areas, especially in the financial markets. This certainly leads to changes in the functioning of this sector. Hence the appearance of new HR practices. The main objective of this research is to see if the talent management may be required in Moroccan companies as a new practice of HRM. To do this, we will first determine the current policy implemented by banks in terms of HRM, then we will try to discern the place of the new trend "Talents" in them, and finally, we will analyze the results and confirm or refute the hypotheses that have been mad of our empirical study. The results of this work may be of practical recommendations to Moroccan banks to review their HR policies, and cope the current issues.

The theoretical part of this thesis describes internal and external ways that can be of use while appointing employees. The thesis is focused on using direct systematic search (Executive Search) in the environment of consulting company CSP Partners Ltd. and its aim is to show the specific features of this method usually used to appoint specialists or managers. Commercial and executive processes of the company are depicted within the text. Using analytical tools this thesis examines the whole HR consulting industry in the context of Czech labor market. Historical development and recent trends are included, ethical dimension as well as motivation of candidates approached using this method are described as well. Many practical notes are involved and the final part describes one of CSP Partners' Ltd. projects in detail.

This thesis deals with talent management. Its aim is to identify the best practices in talent management based on secondary and primary research of selected talent management programs. The theory is focused on definition of strategic HR management and especially talent management as one of its priorities. It is followed by explanation of the basic concepts of talent (such as high-potential, high performing and key employees) and talent management. Further descriptions of talent management process as well as TM challenges are included. The practical part is divided into two main sections. The first one is related to research in four companies and evaluation of their current talent management programs. Due to the fact that Generation Y is still more and more included in talent pool, the second part covers description of work life from its point of view. The final section contains a summary and the best practices.

This diploma thesis concentrates on relationship between employee satisfaction and usage of new methods in Human Resources Management, particularly coaching and talent management. As employee satisfaction becomes one of the most important sources of influence on company's performance, it is also discussed in the theoretical background the relationship between employee satisfaction and company performance. The goal of the thesis is to find relationship between usage of new HRM methods (coaching and talent management) and employee satisfaction. The thesis has four chapters. First two chapters "Measurement Systems in an Organization" and "Methods in HR Management" set a theoretical background for the research. The third chapter concentrates on the research itself: employees of five Russian companies both with and without coaching and talent management, are tested. In the last chapter are compared research outcomes and theoretical approach, as well as, recommendations are given.