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Targeting novel soil glycosyl hydrolases by combining stable isotope probing and metagenomicsVerastegui Pena, Yris Milusqui 14 February 2014 (has links)
Soil represents the largest global reservoir of microbial diversity for the discovery of novel genes and enzymes. Both stable-isotope probing (SIP) and metagenomics have been used to access uncultured microbial diversity, but few studies have combined these two methods for accessing the biotechnological potential of soil genetic diversity and fewer yet have employed functional metagenomics for recovering novel genes and enzymes for bioenergy or bioproduct applications. In this research, I demonstrate the power of combining functional metagenomics and SIP using multiple plant-derived carbon substrates and diverse soils for characterizing active soil bacterial communities and recovering glycosyl hydrolases based on gene expression. Three disparate Canadian soils (tundra, temperate rainforest and agricultural) were incubated with five native carbon (12C) or stable-isotope labelled (13C) carbohydrates (glucose, cellobiose, xylose, arabinose and cellulose). Sampling at defined time intervals (one, three and six weeks) was followed by DNA extraction and cesium chloride density gradient ultracentrifugation. Denaturing gradient gel electrophoresis (DGGE) of all gradient fractions confirmed the recovery of labeled nucleic acids. Sequencing of original soil samples and labeled DNA fractions demonstrated unique heavy DNA patterns associated with all soils and substrates. Indicator species analysis revealed many uncultured and unclassified bacterial taxa in the heavy DNA for all soils and substrates. Among characterized taxa, Salinibacterium (Actinobacteria), Devosia (Alphaproteobacteria), Telmatospirillum (Alphaproteobacteria), Phenylobacterium (Alphaproteobacteria) and Asticcacaulis (Alphaproteobacteria) were the bacterial ???indicator species??? for the heavy substrates and soils tested. Both Actinomycetales and Caulobacterales (genus Phenylobacterium) were associated with metabolism of cellulose. Members of the Alphaproteobacteria were associated with the metabolism of arabinose and members of the order Rhizobiales were strongly associated with the metabolism of xylose.
Annotated metagenomic data suggested diverse glycosyl hydrolase gene representation within the pooled heavy DNA. By screening only 2876 inserts derived from the 13C-cellulose heavy DNA, stable-isotope probing and functional screens enabled the recovery of six clones with activity against carboxymethylcellulose and methylumbelliferone-based substrates.
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以變異數比率法檢定指數選擇權之買賣權平價理論——馬可夫狀態轉換模型之應用秦秀琪 Unknown Date (has links)
本研究目的在於探討Put-Call Parity(PCP)所隱含的買權、賣權與標的資產間的價格變動關係。藉由探討PCP偏差程度的動態行為,推論若PCP的偏差為隨機漫步過程,則無法達到長期穩定,隱含PCP的廣義關係無法成立;反之,若PCP的偏差具有回歸平均特性,表示長期會達到穩定狀態,則PCP的廣義關係成立。
在研究方法上本文以變異數比率法檢定指數選擇權的PCP偏差是否為隨機漫步過程,採用隱含利率和實際無風險利率的差代表PCP的偏差程度,利用馬可夫轉換模型描繪PCP偏差的動態行為,並使用Gibbs Sampling演算法說明參數的不確定性。
本文以S&P500和DAX為研究標的,並探討股利不確定性是否影響PCP廣義關係,得到下列結論:
1、 對於S&P 500指數選擇權而言,不論是以日資料或週資料估計VR,S&P 500的PCP偏差都無法提供回歸平均的證據,隱含S&P 500的PCP廣義關係無法成立。
2、 對於DAX指數選擇權而言,檢定日資料的結果發現,DAX之PCP偏差在長期時(40~50日)有明顯的回歸平均的證據;而在檢定週資料時,使用原始資料法在90%信心水準下,不論取任何lag都可拒絕虛無假設,使用標準化資料則無法提供明顯的回歸平均證據。
3、 比較S&P 500和DAX,檢定日資料與週資料的結果都發現,DAX的p-value都比S&P 500小,並且S&P 500的PCP偏差都無法提供回歸平均的證據,而DAX有明顯回歸平均現象,隱含在消除股利的不確定性後,指數選擇權PCP的廣義關係式成立之證據較強烈。
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En undersökning av kvantiloptioners egenskaperLundberg, Robin January 2017 (has links)
Optioner säljs och köps idag flitigt av många olika anledningar. En av dessa kan vara spekulation kring framtida händelser för aktiepriser där optioner har fördelar jämfört med aktier i form av en hävstångseffekt. En annan anledning för optionshandel är för att hedga (säkra) risker vilket ställer krav på att innehavet av optionen ska kompensera den negativa effekt som riskerna bidrar till. Med andra ord, om det finns en risk för ett negativt framtida scenario som man inte vill riskera att utsätta sig för så kan optioner vara rätt verktyg att använda sig av. Risker finns idag överallt, i olika former, vilket har bidragit till att efterfrågan av optioner har ökat enormt de senaste årtiondena. Dock kan risker vara både komplexa och varierande vilket har lett till att mer komplexa optioner har utvecklats för att mätta den efterfrågan som utvecklats på marknaden. Dessa, mer komplexa optioner, kallas exotiska optioner och de skiljer sig från de vanliga europeiska och amerikanska köp- och säljoptionerna. Däribland hittar vi bland annat lookback-optioner i form av bland annat köpoptioner på maximum och kvantiloptioner vilka är två av de huvudsakliga optionerna som diskuteras i uppsatsen. Det har länge varit känt hur man prissätter europeiska köp- och säljoptioner via Black-Scholes-Mertons modell men desto fler komplexa optioner som tillkommer på marknaden desto mer komplicerade prissättningsmodeller utvecklas. Till skillnad från europeiska köp- och säljoptioner vars utdelning beror på aktiepriset på lösendagen så är lookback-optioner beroende av aktieprisets rörelse under hela kontraktstiden. Detta medför att prissättningen av dessa beror av fler parametrar än i Black-Scholes-Mertons modell, bland annat ockupationstiden för den stokastiska process som beskriver aktiepriset, vilket bidrar till andra prissättningsmodeller. Uppsatsen har som syfte att redogöra för modellen som används vid prissättningen av kvantiloptioner samt presentera hur deras egenskaper förhåller sig till andra typer av lookback-optioners egenskaper. Det presenteras i rapporten att kvantiloptioner liknar vissa typer av lookback-optioner, mer bestämt köpoptioner på maximum, och att kvantiloptioners egenskaper faktiskt konvergerar mot köpoptioner på maximums egenskaper då kvantilen närmar sig 1. Utifrån detta resonemang så kan det finnas fördelar i att använda kvantiloptioner snarare än köpoptioner på maximum vilket investerare bör ta i hänsyn när, och om, kvantiloptioner introduceras på marknaden. / Options are today used by investors for multiple reasons. One of these are speculation about future market movements, here ownership of options is advantageous over usual ownership of shares in the underlying stock in terms of a leverage effect. Furthermore, investors use options to hedge different kinds of risks that they are exposed to, this demands that the option compensates the possible negative effect that the risk brings to the table. In other words, if there is a risk of a future negative scenario which the investor is risk averse to, then owning specific options which neutralize this risk could be the perfect tool to use. Risks are today seen all over the market in different shapes which have created a great demand for options over the last decades. However, since risks can be both complex and range over multiple business areas, investors have demanded more complex options which can neutralize the risk exposures. These, more complex options, are called exotic options, and they differ from the regular American and European options in the way they behave with respect to the underlying stock. Amongst these exotic options, we can find different kind of lookback options as well as quantile options which are two of the main options that are discussed in this thesis. It has been known for a while how to price European call and put options by the Black-Scholes-Merton model. However, with more complex options also comes more complex pricing models and unlike the European options’ payoff which depend on the underlying stock price at time of maturity, the lookback option’s and quantile option’s payoff depend on the stock price movement over the total life span of the option contract. Hence, the pricing of these options depends on more variables than the classic Black-Scholes-Merton model include. One of these variables is the occupation time of the stochastic process which describes the stock price movement, this leads to a more complex and extensive pricing model than the general Black-Scholes-Merton’s model. The objective of this thesis is to derive the pricing model that is used for quantile options and prove that the properties of quantile options are advantageous when compared to some specific lookback options, viz. call options on maximum. It is concluded in the thesis that quantile options in fact converges to the call option on maximum for quantiles approaching 1. However, quantile options come with some different properties which potentially makes them a good substitute for the call option on maximum. This is a relevant factor for investors to consider when, and if, quantile options are introduced to the market.
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Pilgrimage in war : the influence of the Second World War and the theme of vocation in Evelyn Waugh's later novels / 戦火の歴拝 : イーヴリン・ウォーの後期小説における第二次世界大戦の影響と召命のテーマ / センカ ノ レキハイ : イーヴリン ウォー ノ コウキ ショウセツ ニオケル ダイニジ セカイ タイセン ノ エイキョウ ト ショウメイ ノ テーマ / 戦火の歴拝 : イーヴリンウォーの後期小説における第二次世界大戦の影響と召命のテーマ有為楠 香, Kaori Wicks 13 September 2018 (has links)
本論文はイギリス20世紀のカトリック作家イーヴリン・ウォー(Evelyn Waugh)(1903-66)の後期作品、主に1940-1960年代に書かれた小説について論じるものであり、とりわけ、彼の最後の作品である『名誉の剣』三部作(the Sword of Honour trilogy)を中心に考察する。本論の考察の目的は、作品が書かれた時代のイギリス社会とウォーの作品との関連性、そして彼が希求した、キリスト教徒としての召命のテーマを探ることである。 / This dissertation is on Evelyn Waugh's (1903-66) later novels, written from 1942, through the Second World War, to 1965, especially on his last ones, the Sword of Honour trilogy. With discussions focusing on the relationship of Waugh's works with British society of the same period, this thesis clarifies the theme of vocation, which is observed in most of his novels. / 博士(英文学) / Doctor of Philosophy in English Literature / 同志社大学 / Doshisha University
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FauxtopiaKampf, Raymond William 01 January 2004 (has links)
To all who come to this fictitious place:Welcome.Fauxtopia is your land. Here, age relives distorted memories of the past, and here, youth may savor the challenge of trying to understand the present. Fauxtopia is made up of the ideals, the dreams and the fuzzy facts which have re-created reality... with the hope that it will be a source of edutainment for all the world.Ray KampfFauxtopia DedicationApril 1st, 2004
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A promise kept: the mystical reach through lossCollins, Jody 04 October 2019 (has links)
The meaning of loss is love. I know this through attention to experience. Whether loss or love is experienced in abundance or in absence, the meaning is mystical with an opening of body, mind, heart and soul to spirit. And so, in the style of a memoir, in the way of contemplative prayer, I contemplate and share my soul as a promise kept in the mystical reach through loss. With the first, initiating loss, the loss of my nine-year-old nephew, Caleb, I experience an epiphany that gives me spiritual instructions that will not be ignored. I experience loss as an abundance of meaning that comes to me as gnosis, as “knowledge of the heart” according to Elaine Pagels or divine revelation in what Evelyn Underhill calls mystical illumination in the experience of “losing-to-find” in union with the divine. Then, with gnostic import, in leaving the ordinary for the extraordinary, I enter the empty room in the painful yet liberating experience of the loss of my self. In the embrace of emptiness, I proceed to the first wall, the second wall, the third wall, the dark corner of denial, the return to centre, and, finally, to breaking the fourth wall in the empty room so as to keep my promise to you. Who are “you”? You are God. You are Caleb. You are spirit. You are my higher soul or self. And, you are the reader. You are my dear companion in silence. And then, through a series of broken promises and more loss, within what John of the Cross calls, “the dark night of the soul,” I am stopped by the ineffability of the dark corner of denial, the horror of separation and the absence of meaning, which is depicted as the grueling gap between the spiritual abyss and the breakthrough. What does it mean to keep going through a solemn succession of losses? I don’t know. In going into the empty room, I simply put pain to work in order to reach you. Through loss, though there are infinite manifestations, there is only one way: keep going. And so, in a triumph of the spirit, I keep going so as to be: a promise kept in the mystical reach through loss. As for you, through my illumined and dark experiences of loss, what is my promise to you? I keep going to reach the unreachable you. In the loss of self, with embodied emptiness, in going into the dark corner of denial, with a return to the divine centre of my emptied self, in an invitation to you, I give my soul to you in union with you. / Graduate / 2020-06-25
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Incidencia del derecho en la creación y funcionamiento de las Joint Ventures. Análisis de la organización jurídica de las Joint Ventures en la Unión Europea, con especial referencia a EspañaPauleau, Christine 17 November 2000 (has links)
La trascendencia práctica del tema del régimen jurídico de las joint ventures es indudable. En los Estados miembros de la Unión Europea, y más especialmente en España, las joint ventures remiten a una idea de mayor eficiencia económica, permitiendo a las empresas formar alianzas entre ellas, y así mejorar su competitividad, adaptando sus estructuras a las dimensiones crecientes de los mercados europeos y mundiales.Las joint ventures no están reguladas como tales en ninguna legislación nacional europea. El derecho comunitario se concentra por su parte en el análisis de los efectos de estas operaciones sobre la competencia. Las joint ventures adquieren en la práctica, únicamente, su contenido jurídico.Este estudio tiene por objetivo presentar un análisis a la vez teórico y práctico del régimen jurídico de las joint ventures, intentando definir el "valor añadido" del trabajo del jurista profesional cuando interviene en la creación y el funcionamiento de las joint ventures. La adopción de esta óptica funcionalista permite integrar el análisis de los diversos aspectos jurídicos de las joint ventures, sus estructuras contractuales y societarias así como su tratamiento en derecho de la competencia, que se abordan tradicionalmente en estudios separados, e insistir en la interelación existente entre las diversas ramas del derecho afectadas. Este estudio se concentra esencialmente en las cuestiones planteadas por las joint ventures en el ámbito del derecho de las obligaciones y del derecho societario.El jurista profesional desempeña, en primer lugar, una función de naturaleza organizativa, creando un conjunto de reglas obligatorias (reglas jurídicas) para todas las empresas participantes en la operación, de acuerdo con sus intereses estratégicos. El resultado puede ser, por ejemplo, la conclusión de un simple contrato, la constitución de una AEIE o la de una sociedad de capital. La complejidad de la organización jurídica de la joint venture impide estudiar de manera separada, como dos piezas aisladas, el llamado acuerdo de base de la joint venture por un lado, y la sociedad eventualmente constituida, la llamada filial común, por otro. El hecho de no entender la filial común como parte de un todo unitario y más complejo no permite describir correctamente las especialidades del régimen aplicable a dicha sociedad frente a sociedades constituidas en otros contextos económicos. La organización compleja de las joint ventures se diferencia de otros negocios jurídicos complejos por razón de su finalidad económica específica, la de establecer una alianza entre empresas. Tal como indica el derecho comunitario de la competencia, la joint venture es una operación en la que: (i) participan dos o más empresas independientemente activas en el mercado; (ii) se agrupan recursos necesarios al desarrollo de una actividad económica común en el seno eventualmente, pero no obligatoriamente, de una entidad separada (la llamada empresa común"); (iii) las empresas participantes ejercen un control conjunto sobre dicha actividad económica común. Estos tres elementos forman el núcleo de la definición de la joint venture en derecho.La cuestión de la validez y eficacia jurídica de los pactos presentes en el contrato complejo de joint venture, cuando implica la existencia de una sociedad de capital, es la que más dificultades plantea. El problema respecto a la joint venture, como respecto a muchos otros contratos complejos, es organizar un discurso sobre el uso de la libertad y sus consecuencias en el ámbito del derecho de sociedades.Mientras el jurista profesional organiza la joint venture, atrae también la atención del legislador sobre las necesidades jurídicas de las empresas que deciden "aliarse" en el seno de una joint venture. Los pactos presentes en los contratos complejos de joint venture no dependen únicamente del contenido del derecho aplicable a la sociedad "filial común" por ejemplo, sino también de la creatividad de los juristas profesionales. Las finalidades a cubrir por el contrato lo serán en regla general mediante combinaciones originales y complejas de mecanismos jurídicos, demostrándose una vez más el papel relevante de la práctica en la formación del derecho. Como consecuencia, puede observarse una tendencia a la flexibilización del derecho aplicable a las sociedades de capital, sobre todo en los países europeos continentales. Esta adaptación del derecho a la realidad práctica se observa también, y de manera significativa, en otras ramas del derecho, como en derecho de la competencia donde el legislador intenta introducir un tratamiento cada vez más pragmático de los efectos producidos por las joint ventures sobre los mercados. El análisis de la organización jurídica de las joint ventures en la práctica demuestra, sin embargo, la falta de utilidad y de oportunidad de una ordenación legal de la joint venture como tal en Europa. Como en el caso de la organización de la empresa, basta con que los operadores "candidatos" a la alianza encuentren en el derecho positivo los legal tools, o instrumentos jurídicos, adaptados a los objetivos económicos legítimos que persiguen ( por ejemplo, diversas formas sociales, la AIE, la UTE, la cuenta en participación etc), y sobre la base de los cuales puedan conservar y reforzar el sentimiento de confianza que necesitan tener en la operación y en su "aliado" para alcanzar con éxito estos objetivos. / This study provides a comprehensive analysis of both practical and theoretical legal issues raised by joint ventures in Europe. In the European Union member states and especially in Spain, joint ventures are viewed as a high efficient strategic operation - allowing alliances between firms, which may improve their competitiveness and adapt them to the growing dimensions of European markets and to the new global economy. Joint ventures are not organised as such by any statute law in Europe. European law focuses on the analysis of the effects they may produce on competition. Joint ventures obtain a legal content only in practice.The aim of this study is to define the "added value" of the practical work of lawyers, when they take part in processes of creation of joint ventures. This question allows to integrate the analysis of different legal aspects of joint ventures - their contractual and corporate structure as well as their legal treatment under competition law - which are traditionally treated in separate studies, and to point out the existence of on-going interfaces between them. This study focuses essentially on the contractual and corporate aspects of joint ventures. Practitioners set up, first of all, mechanisms which are compulsory - i.e. legal rules - for the firms involved in the operation according to their strategic interests. The result may be, for instance, the creation of a mere "contractual joint venture", of an European Economic Interest Grouping, or the incorporation of a "joint venture company". The complexity of the joint venture legal organisation prevents from studying separately the so-called "joint venture agreement" on which is based the operation, and the company which may be incorporated within it. The joint venture company is only a part of a more complex ensemble, which needs to be understood as such in order to be correctly analysed.The complex legal organisation of joint ventures differs from any other complex legal organisations because of its specific purpose, ie. establish a strategic alliance between firms. It aims to organise an operation in which: (i) participate two or more firms independently active on the market; (ii) are gathered the resources necessary to carry out a common business activity, possibly but not obligatory within a separate entity; and (iii) the participating firms jointly control the common business activity. Those three features are at the basis of the legal definition of joint ventures.The question of the legal validity and efficiency of the terms and conditions present in incorporated joint venture agreements is the more problematic one for the practitioners. The main difficulty in relation with joint ventures like in relation with many other complex agreements is to determine the limits of use of contractual freedom in company law.While organising the operation, the practitioners are also drawing the attention of the legislator on the legal needs of the firms willing to enter into alliances. The terms and conditions present in joint venture agreements depend not only on the content of the statute law applicable to the joint venture company for example, but also on the creative skills of the practitioners. The joint venture agreement needs to cover different purposes generally through original and complex combinations of legal mechanisms, proving once again the relevant role of practice in the generation of law.As a result, it is possible to observe a certain "flexibilization" of the rules in company law, especially in continental Europe. This new tendency develops in parallel with the movement observed in competition law towards a more realistic and pragmatic analysis of joint venture effects on the markets.The analysis of the legal organisations of joint ventures existing in practice demonstrates however the lack of necessity for a statutory regulation of this (strategic) operation in Europe. Like in the case of the organisation of a firm, the economic players willing to enter into strategic alliances only need to find in statute law different "legal tools" which may adapt their legitimate economic interests - such as different company forms, the Economic Interest Grouping, the partnership - and on the basis of which they can conserve and reinforce the confidence they need to have in their partner and in the operation in order to achieve successfully their purposes.
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Bullying: Out Of The School Halls And Into The WorkplaceCooney, Lucretia 01 January 2010 (has links)
The primary purpose of this study is to identify those people at most risk of being bullied at work. While much research is being conducted on school bullying, little has been conducted on workplace bullying. Using data gathered from a 2004 study conducted by the National Opinion Research Center for the General Social Survey, which included a Quality of Work Life (QWL) module for the National Institute for Occupational Safety and Health (NIOSH), linear regressions indicated significant findings. As predicted, workers in lower level occupations, as ranked by prestige scoring developed at National Opinion Research, are more likely to be victimized. Data also suggest that being young, Black, and relatively uneducated may contribute to being bullied in certain situations. Future research is needed to examine influences of socio-economic, legal, and other demographic factors that may predict the chance of being bullied.
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INTERTEXTUALIZAÇÃO NA OBRA DE MARINA COLASANTI: O TEAR E O TECIDOCosta, Ivonete Ferreira da 23 March 2016 (has links)
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Previous issue date: 2016-03-23 / The text brings the analysis of aspects of the literary discourse as the processes of
construction of the scenes and the magical universe, in which the narratives of
Marina Colasanti are realized, having as it shows the tales of the works Doze reis e a
moca no labirinto do vento (2006): "The woman ramada", Uma ideia toda azul
(2006): "Beyond the frame", "Between the leaves of green ó" and "Yarn after yarn".
The general and specific objectives are to highlight and distinguish the
encompassing and generic scenes present in the narratives, to identify the nature of
the verbal sign in its relation to the nonverbal sign, and to analyze intertext
resources, paratext, among others, as an artistic procedure. The narrative plans are
approached, in which the characters are realized mimically, starting from the initial
assumption formulated by Dominique Maingueneau. Non-verbal language is an
invitation to read verbal language and vice versa. Both are associated with the signs
that are constructed through the textual writing: loom and fabric. They can be seen
now either explicitly or implicitly, and put in the service of a power that is realized by
the act of reading. Thus, in the narrative text, there are traces of a speech in which
the text is staged. / O texto traz a análise de aspectos do discurso literário como os processos de
construção das cenas e o universo mágico, em que se realizam as narrativas de
Marina Colasanti, tendo como mostra os contos das obras Doze reis e a moca no
labirinto do vento (2006): “A mulher ramada”, Uma ideia toda azul (2006): “Além do
bastidor”, “Entre as folhas do verde ó” e “Fio após fio”. Os objetivos geral e
específicos são destacar e distinguir as cenas englobante e genérica presentes nas
narrativas, identificar a natureza do signo verbal na sua relação com o signo não
verbal e analisar recursos de intertexto, paratexto, entre outros, como procedimento
artístico. Abordam-se os planos narrativos, nos quais se dá a realização dos
personagens mimeticamente, partindo do pressuposto inicial formulado por
Dominique Maingueneau. A linguagem não verbal é um convite à leitura da
linguagem verbal e vice-versa. Ambas se associam aos signos que se constroem
por meio da escritura textual: tear e tecido. Elas podem ser vistas ora de modo
explícito, ora implícito, e se colocam a serviço de um poder que se realiza pelo ato
de leitura. Assim, no texto narrativo, há rastros de um discurso em que o texto é
encenado.
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Pomocná brzda pro eskalátor / Auxiliary brake of escalatorLacheta, Lukáš January 2008 (has links)
Purpose of engaged task is elaborate a functional mechanism to emergency brake for escalators company Otis. To this purpose is gradually reached up in several phases. In primary phase was taken view on similar products from competitive company and do not get in conflict with patent right, in second phase was needed delimitate working space for brake and exact specification of straining. In next steps the proposal and test phase is blending together. In this moment emergency brake has own shape and first tests are showing the functions, but as well necessity modification of same elements on brake and on escalator.
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