Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T13:30:02Z No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T13:30:22Z (GMT) No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-06T13:30:40Z (GMT) No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-01-06T13:30:40Z (GMT). No. of bitstreams: 2 inae_marie_araujo_silva.pdf: 2900695 bytes, checksum: b0f455759c609f430fbe59626102155a (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2013 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Atualmente, devido as limita??es de expans?o das tradicionais ?reas de plantio de eucalipto, v?rios grupos de pesquisa t?m buscado estrat?gias para ampliar as fronteiras florestais brasileiras. Entretanto, a baixa disponibilidade h?drica dessas regi?es fronteiri?as tem limitado o desenvolvimento de alguns gen?tipos de eucalipto. ? vista disso, objetivou-se: i) avaliar as respostas morfofisiol?gicas de dois gen?tipos de eucalipto contrastantes quanto ? suas capacidades de tolerar o d?ficit h?drico e ii) construir uma biblioteca g?nica diferencial do gen?tipo tolerante ? seca, de maneira a possibilitar ? compreens?o dos mecanismos subjacentes ? toler?ncia ao d?ficit h?drico do ponto de vista gen?tico-molecular. O experimento foi conduzido, em casa de vegeta??o na UFVJM, em Diamantina, MG. As plantas dos dois gen?tipos, clones 953 (Eucalyptus camaldulensis vs. Eucalyptus grandis) e 224 (Eucalyptus grandis vs. Eucalyptus urophylla), modelo de toler?ncia e sensibilidade ao d?ficit h?drico, respectivamente, foram aclimatadas em casa de sombra, com irriga??o, durante 30 dias. Ap?s esse per?odo, foram submetidos a dois diferentes regimes h?dricos (irrigado e n?o irrigado), quando, ent?o, foram avaliados, ao longo do per?odo experimental, o crescimento em altura e di?metro, ?rea foliar, ?ndice de clorofila total, vari?veis da fluoresc?ncia da clorofila a, vari?veis de trocas gasosas, mat?ria seca de todos os compartimentos da planta e suas rela??es derivadas (rela??o mat?ria seca raiz e parte a?rea, raz?o de massa foliar, ?rea foliar espec?fica e raz?o de ?rea foliar), al?m da sintomatologia do estresse. O experimento foi conduzido em um Delineamento Inteiramente Casualizado, em esquema fatorial 2 (gen?tipos) x 2 (regimes h?dricos), totalizando quatro tratamentos (25 repeti??es cada). As an?lises estat?sticas foram realizadas mediante an?lise de vari?ncia. Para o estudo da express?o g?nica, concomitantemente a aplica??o do estresse h?drico, folhas foram coletadas, congeladas em N2 l?quido e armazenadas a - 80 ?C. Em seguida, procedeu-se com as extra??es e purifica??es do RNA total e, posterior, constru??o da biblioteca subtrativa. A defici?ncia h?drica n?o limitou o crescimento em altura dos gen?tipos estudados, embora, tenha reduzido o di?metro e a produ??o de mat?ria seca total. As demais vari?veis morfofisiol?gicas e todas as vari?veis de trocas gasosas foram negativamente afetadas pelo d?ficit h?drico. O clone 224 mostrou-se menos eficiente em termos fotossint?ticos e mais sens?vel ? restri??o h?drica para a maioria das vari?veis analisadas. Em contrapartida, o 953 apresentou maior toler?ncia ? falta d'?gua. Possivelmente, ?rea foliar reduzida, aumentos no teor de clorofila e aparelho fotossint?tico mais eficiente podem ter sido determinantes nessa maior toler?ncia encontrada no clone 953. Essas estrat?gias, podem, portanto, serem alvos em programas de sele??o de materiais gen?ticos mais tolerantes ? seca. Nesse sentido, a efici?ncia qu?ntica do Fotossistema II mostrou-se uma caracter?stica a ser considerada num processo de sele??o para toler?ncia ? falta d'?gua. A biblioteca de cDNA constru?da permitir? identificar quais genes est?o envolvidos nesse processo de toler?ncia no gen?tipo 953. Futuramente, esses genes candidatos poder?o ser transferidos para gen?tipos de interesse econ?mico, por meio de transgenia. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Currently, due to the limitations of expanding areas traditional of eucalyptus, several research groups have pursued strategies to expand the boundaries Brazilian forest. However, the low water availability of these border regions has limited the development of some genotypes of eucalyptus. Therefore, we aimed to: i) evaluate the morphological and physiological responses of two contrasting genotypes of eucalyptus as to their ability to tolerate drought and ii) build a library differential gene genotype drought tolerant, in order to enable the understanding molecular mechanisms underlying tolerance to water deficit. The experiment was conducted in a greenhouse at UFVJM in Diamantina, MG. The plants of the two genotypes, 953 clones (Eucalyptus camaldulensis vs. Eucalyptus grandis) and 224 (Eucalyptus grandis vs Eucalyptus urophylla) model of tolerance and sensitivity to water stress, respectively, were acclimated in the shade, irrigated for 30 days. After this period, underwent two different water regimes (irrigated and non-irrigated), when then were evaluated over the experimental period, the growth in height and diameter, leaf area, total chlorophyll index, chlorophyll a fluorescence variables, gas exchange variables, dry matter of all plant parts and their relations derived (shoot/root dry weight ratios, leaf weight ratio, specific leaf area and leaf area ratio) and the symptoms of stress. The experiment was conducted in a completely randomized design in a factorial 2 (genotypes) x 2 (water regimes), totaling four treatments (25 replicates each). Statistical analyzes were performed by analysis of variance. For the study of gene expression, concomitant application of water stress, leaves were collected, frozen in liquid N2 and stored at - 80 ?C. Then proceeded with the extraction and purification of total RNA and subsequent construction of the subtractive library. Water stress did not limit the growth in height of the genotypes studied, although it reduced the diameter and total dry matter production. The other morphophysiological variables and all gas exchange variables were negatively affected by drought. Clone 224 was less efficient photosynthetic and more sensitive to water restriction for most variables. In contrast, the 953 was more tolerant to lack of water. Possibly, reduced leaf area, increases in chlorophyll content and photosynthetic apparatus more efficient may have been determinant to increase tolerance found in clone 953. These strategies can be targeted in selection programs of genetic materials more drought tolerant. In this sense, the quantum efficiency of Photosystem II showed a characteristic to be considered in the selection process for tolerance to water shortage. A cDNA library constructed will allow to identify which genes are involved in the process of tolerance on the genotype 953. In the future, these candidate genes may be transferred to genotypes of economic interest through transgenesis.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:44Z No. of bitstreams: 5 patricia.pdf: 1377961 bytes, checksum: 55ce7c1439d8b1123461e7f1f40d7a17 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T10:38:49Z (GMT) No. of bitstreams: 5 patricia.pdf: 1377961 bytes, checksum: 55ce7c1439d8b1123461e7f1f40d7a17 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T10:38:49Z (GMT). No. of bitstreams: 5 patricia.pdf: 1377961 bytes, checksum: 55ce7c1439d8b1123461e7f1f40d7a17 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / Os estudos sobre o desenvolvimento de defeitos de esmalte e a associa??o com as condi??es natais e p?s-natais ainda s?o escassos. Assim, este estudo teve como objetivo avaliar a preval?ncia de defeitos de esmalte em dentes dec?duos e verificar se prematuridade, peso ao nascimento e vari?veis sociodemogr?ficas podem estar associados a tais defeitos. Foi realizado um estudo transversal com 381 crian?as com idade entre 3 e 5 anos. Os dados foram coletados atrav?s de exame cl?nico e entrevistas com os pais durante Campanha Nacional de Vacina??o. O diagn?stico dos defeitos de esmalte foi realizado utilizando-se o Developmental Defects of Enamel Index (DDE Index). Atrav?s de entrevista foram coletadas informa??es sobre aspectos sociodemogr?ficos, gesta??o, peso ao nascimento, prematuridade da crian?a e amamenta??o. A an?lise dos dados foi realizada utilizando-se o programa SPSS for Windows 17.0 e incluiu a distribui??o de frequ?ncia, testes qui-quadrado e Exato de Fisher e regress?o de Poisson. A preval?ncia de defeito de esmalte foi de 29,9% e a opacidade demarcada foi o tipo de defeito mais frequente. Crian?as com peso muito baixo ao nascimento apresentam mais chance de desenvolver defeitos de esmalte [RP (IC95%) = 2,7 (1,66-4,61)]. A prematuridade e as vari?veis sociodemogr?ficas n?o foram fatores associados aos defeitos de esmalte. Crian?as com peso muito baixo ao nascimento apresentaram maior preval?ncia de defeitos de esmalte nos dentes dec?duos. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT Studies regarding the development of enamel defects and association with natal and postnatal conditions are scarce. Thus, the aim of this study was to evaluate the prevalence of enamel defects in primary teeth and determine whether prematurity, birth weight and sociodemographic variables may be associated with such defects. This was a cross-sectional study with 381 children aged between 3 and 5 years. Data were collected through clinical examination and interviews with parents during the National Immunization Day. The diagnosis of enamel defects was performed using the Development Defects of Enamel Index (DDE Index). During interviews, information were collected on sociodemographic aspects, pregnancy, birth weight, child prematurity and breastfeeding. Statistical analysis was performed using the SPSS software for Windows 17.0 and involved descriptive analysis, the Fisher?s test, qui-square test and Poisson regression. The prevalence of enamel defects was 29.9% and demarcated opacity was the most frequent type of defect. Children with low birth weight presented greater prevalence of enamel defects [PR (CI 95%) = 2,7 (1.66-4.61)]. Prematurity and the sociodemographic variables were not factors associated with enamel defects. In this study, children with very low birth weight had higher prevalence of enamel defects in deciduous teeth.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T16:14:42Z No. of bitstreams: 5 carlos.pdf: 1077384 bytes, checksum: 83eb6b248c6848161b2017a683c19682 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-10T11:09:38Z (GMT) No. of bitstreams: 5 carlos.pdf: 1077384 bytes, checksum: 83eb6b248c6848161b2017a683c19682 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-10T11:09:38Z (GMT). No. of bitstreams: 5 carlos.pdf: 1077384 bytes, checksum: 83eb6b248c6848161b2017a683c19682 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2013 / O objetivo do presente estudo foi realizar uma avalia??o comparativa do efeito da dexametasona e da metilprednisolona no controle da dor, edema e limita??o da abertura bucal ap?s a extra??o de terceiros molares impactados. Dezesseis pacientes saud?veis (3 homens e 13 mulheres) com m?dia de 20,3 (DP= 1,25) anos de idade foram submetidos ? extra??o bilateral dos terceiros molares com intervalo de tr?s a quatro semanas entre as duas cirurgias. Uma hora antes de cada procedimento cir?rgico foi administrada em dose ?nica, por via oral, dexametasona 8 mg ou metilprednisolona 40 mg. Durante os intervalos p?s-operat?rios de 24, 48 e 72 horas e sete dias, o edema linear foi determinado utilizando medidas na face e o trismo atrav?s da abertura bucal m?xima. A dor p?s-operat?ria foi auto-registrada pelo paciente utilizando escala anal?gica visual durante as 72 horas iniciais, em intervalos de oito horas. A an?lise estat?stica envolveu an?lise descritiva, teste McNemar, Teste T pareado e Wilcoxon (p<0,05). A dexametasona apresentou melhor controle do edema em todos os per?odos p?s-operat?rios (p <0,02) e da abertura bucal dois dias ap?s a cirurgia (p = 0,029). Com rela??o ? dor n?o foi observada diferen?a estatisticamente significativa entre os medicamentos. A administra??o pr?-perat?ria de 8 mg de dexametasona apresentou melhor controle do edema e da limita??o da abertura bucal do que a metilprednisolona na dose de 40 mg, muito embora os medicamentos n?o diferiram no controle da dor. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The?aim of this study?was to make a comparative assessment of the effect of?dexamethasone and methylprednisolone?to control pain, swelling?and limitation?of mouth opening after extraction of?impacted third molars.?Sixteen healthy patients (3 men and 13 women)?with a mean age?of 20.3 (SD = 1,25) years underwent bilateral extraction?of?third molars with na interval of three to four weeks between the two surgeries. One hour?before each?surgical procedure?a single oral dose?of?dexamethasone 8?mg or methylprednisolone?40?mg was administered.?During the postoperative?intervals?of 24,?48?and 72?hours and?seven days, the swelling was determined using?linear?measures?in the?face and trismus?by?maximum mouth opening. The?postoperative pain?was self-recorded?by the patient using?visual analogue scale?in?72 hours with an interval of eight hours. Statistical analysis involved descriptive analysis, McNemar test, Wilcoxon?and paired T test (p?<0.05). Dexamethasone showed better control of swelling in all?postoperative periods (p?<0.02) and of mouth opening two days after surgery (p = 0.029). Regarding pain there was no statistically significant difference between the drugs. Preemptive dexamethasone?8 mg showed better control of swelling?and limitation?of mouth opening than methylprednisolone 40 mg, but there was no difference?in pain control.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:03:28Z No. of bitstreams: 5 crisley.pdf: 2081607 bytes, checksum: 90ca0fb9cd507118e713cc82f093637a (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T09:27:10Z (GMT) No. of bitstreams: 5 crisley.pdf: 2081607 bytes, checksum: 90ca0fb9cd507118e713cc82f093637a (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-20T09:27:10Z (GMT). No. of bitstreams: 5 crisley.pdf: 2081607 bytes, checksum: 90ca0fb9cd507118e713cc82f093637a (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-12-17 / A busca por energias alternativas, sustent?veis e economicamente vi?veis, colocou em destaque o uso dos biocombust?veis. Os estudos recentes apontam o uso de biomassas residuais como candidatas potenciais para produ??o de bioenergia. Nesse contexto, a presente disserta??o teve por objetivo ava-liar a torta de dend? como insumo para produ??o de etanol de segunda gera??o por meio de estudos que envolveram etapas de pr?-tratamento ?cido e alcalino, sacarifica??o e fermenta??o. Inicialmente foi determinada a composi??o centesimal da torta de dend?, indicando a presen?a de 53,2% de car-boidratos totais, dos quais 32% referente a fra??o de celulose. Para otimiza??o do pr?-tratamento ?cido foi aplicado um Planejamento Fatorial atrav?s de delineamento composto central rotacional. O programa STATISTICA Vers?o 8.0 (Statsoft Inc., Tulsa,) foi utilizado para an?lise dos dados. O pr?-tratamento com ?cido sulf?rico dilu?do resultou na remo??o de pelo menos 82% da hemicelulose presente na torta de dend?. O pr?-tratamento alcalino subsequente foi capaz de remover pelo menos 72% da lignina da biomassa, um interferente do processo fermentativo. Na sequ?ncia o processo de sacarifica??o foi otimizado com o uso de delineamento composto central rotacional com 3 fatores, 4 pontos centrais e 6 pontos axiais, onde foram avaliadas a concentra??o de celulases, a raz?o s?lido-l?quido e o tempo. O hidrolisado enzim?tico obtido em condi??o otimizada, com 80% de convers?o da celulose a a??cares redutores, foi submetido ? fermenta??o com Saccharomyces cerevisiae apre-sentando um YP/S de 0,49 com 5,4%(v/v) de ?lcool no mosto fermentado. Os dados obtidos indicaram que a torta de dend? avaliada apresenta elevado potencial para produ??o de bioetanol de segunda gera??o. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT The search for alternative energies, sustainable and economically viable, highlighted the use of biofuels. Recent studies indicate the use of residual biomass as potential candidates for bioenergy production. In this context, the present dissertation aimed at evaluating the palm cake as a feedstock for the production of second generation ethanol through studies involving acid and alkaline pretreatment steps, saccharification and fermentation. Initially it was determined the chemical composition of palm cake, indicating the presence of 53.2% of total carbohydrates, of which 32% related to cellulose. To optimize the pretreatment was applied a Factorial Design by central composite rotational design. The program STATISTICA version 8.0 (Statsoft Inc., Tulsa,) was used for data analysis. Pretreatment with dilute sulfuric acid resulted in the removal of at least 82% of the hemicellulose present in palm cake. The subsequent alkaline pretreatment was able to remove at least 72% of the lignin from the biomass, an interfering of fermentation. Further the saccharification process was optimized by the use of central composite design with three factors, fourth central points and six axial points, evaluating the concentration of cellulase, the solid-liquid ratio and the time. The enzymatic hydrolyzate obtained under optimized conditions with 80% conversion of cellulose to sugars was subjected to fermentation with Saccharomyces cerevisiae showing an YP/S of 0.49 based on 5.4% (v/v) of alcohol in the fermented must. The data indicated that the palm kernel cake evaluated presents high potential for production of second generation bioethanol.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-20T12:56:36Z No. of bitstreams: 0 / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: n?o da para inserir licen?a on 2015-01-20T16:55:25Z (GMT) / Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T11:32:34Z No. of bitstreams: 2 bethania_alves_avelar1.pdf: 1374426 bytes, checksum: 09450a40db624b58e0a45e591d96b5d9 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T11:33:27Z (GMT) No. of bitstreams: 2 bethania_alves_avelar1.pdf: 1374426 bytes, checksum: 09450a40db624b58e0a45e591d96b5d9 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-02-20T11:33:27Z (GMT). No. of bitstreams: 2 bethania_alves_avelar1.pdf: 1374426 bytes, checksum: 09450a40db624b58e0a45e591d96b5d9 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2010 / O uso de plantas da fam?lia Euphorbiaceae, principalmente do g?nero Euphorbia, tem sido popularmente difundido para o tratamento de uma variedade de doen?as de natureza infecciosa, tumoral e inflamat?ria. Entre as esp?cies desse g?nero, a Euphorbia tirucalli ? uma planta de uso difundido no Brasil e em algumas regi?es, tal como a do Vale do Jequitinhonha. H? ind?cios de que o l?tex de E. tirucalli tenha atividade antitumoral e antiviral, por?m, pouco se sabe sobre os mecanismos envolvidos nessa a??o. ? prov?vel que o mecanismo de a??o para tais atividades envolva a ativa??o de leuc?citos e a produ??o de citocinas para o direcionamento de uma resposta antiviral e antitumoral efetivas. Diante disso, o presente trabalho avaliou a produ??o de citocinas TNF-?, IFN-?, IL-4 e IL-10 nas popula??es e subpopula??es leuc?cit?rias do sangue perif?rico estimulados com o l?tex bruto da referida planta. Para tal, leuc?citos do sangue perif?rico de vinte indiv?duos h?gidos foram incubados por 4 horas com o l?tex bruto de E. tirucalli dilu?do em Dimetilsulfoxido (DMSO) e constituiu o grupo l?tex (Lt). Culturas celulares incubadas com salina e DMSO, utilizando-se o mesmo per?odo de incuba??o, constitu?ram as culturas controle n?o estimuladas (CC) e controle de solvente (DMSO), respectivamente. Ap?s o per?odo de incuba??o, as c?lulas foram marcadas com anticorpos monoclonais espec?ficos para receptores de superf?cie celular CD4, CD8 e CD14, bem como para as citocinas intracitoplasm?ticas TNF-?, IFN-?, IL-4 e IL-10. A aquisi??o e an?lise dos dados foram realizadas por citometria de fluxo. Os resultados demonstraram um aumento significativo (p < 0,05) no percentual de linf?citos T e sua subpopula??o T CD4 positivos para as citocinas do tipo 1, TNF-? e IFN-?. Essas citocinas s?o respons?veis por ativar o sistema imune para respostas imunoprotetoras frente ?s infec??es virais e processos tumorais. J? os neutr?filos e linf?citos T CD8, nas culturas estimuladas com o l?tex da planta, apresentaram um perfil misto de produ??o de citocinas do tipo 1 e 2. Esses resultados evidenciaram uma resposta do tipo 1 predominante, in vitro, e uma prov?vel ativa??o de mecanismos moduladores, mediados por linf?citos T CD8 e neutr?filos. Este estudo sugere que a utiliza??o popular da planta em infec??es virais e processos tumorais pode ter algum fundamento, tendo em vista a produ??o preferencial de citocinas do tipo 1 em c?lulas estrat?gicas da resposta imune celular, fundamental no combate ?s patologias mencionadas. No entanto, os estudos in vitro aqui realizados n?o s?o suficientes para garantir que a utiliza??o da planta no contexto in vivo possa, de fato, apresentar tais propriedades. Assim, estudos adicionais, incluindo outros ensaios in vitro, bem como ensaios pr?-cl?nicos, s?o fundamentais para o avan?o das pesquisas envolvendo a utiliza??o de E. tirucalli para fins terap?uticos. / ABSTRACT The use of plants in the family Euphorbiaceae, especially of the genus Euphorbia, has been popularly circulated for the treatment of a variety of infectious, tumoral and inflammatory illnesses. Among the species of this genus, Euphorbia tirucalli is a the plant with widespread use in Brasil and in some regions, such as the Vale do Jequitinhonha. There is evidence that the latex of E. tirucalli has antiviral and antitumor activity, but little is known about the mechanisms involved in this action. It is likely that the mechanism of action for such activities involves leukocyte activation and cytokine production for directing an effective antitumor and antiviral response. Thus, the present study evaluated the production of cytokines TNF-?, IFN-?, IL-4 and IL-10 in the populations and subpopulations in peripheral blood leukocytes stimulated with crude latex of this plant. For such, peripheral blood leukocytes of twenty healthy subjects were incubated for 4 hours with crude latex of E. tirucalli diluted in dimethyl sulfoxide (DMSO) and was the latex group (Lt). Cell cultures incubated with saline and DMSO, using the same incubation period, represent the control cultures that were not stimulated (CC) and solvent control (DMSO), respectively. After the incubation period, cells were stained with monoclonal antibodies specific to cell surface receptors CD4, CD8 and CD14, as well as for TNF-? intracytoplasmic cytokines, IFN-?, IL-4 and IL-10. The acquisition and analysis were performed by flow cytometry. The results showed a significant increase (p <0.05) in the percentage of T lymphocytes and their subpopulation T CD4 positive for type 1 cytokines, TNF-? and IFN-?. These cytokines are responsible for activating the immune system to immunoprotective responses against viral infections and tumor processes. However the neutrophils and T CD8 lymphocytes, in cultures stimulated with the latex of the plant, showed a mixed profile of cytokine production of type 1 and 2.These results showed a response of the type 1 predominant, in vitro, and a probable activation of modulatory mechanisms mediated by T CD8 lymphocytes and neutrophils.This study suggests that the popular use of plant in viral infections and tumor processes may have some foundation, in order to preferential production of type 1cytokines in strategic cells of the cellular immune response, that is essential in combating the diseases mentioned. However, in vitro studies conducted here are not sufficient to ensure that the use of the plant in vivo context may, in fact, possesses such properties. / Disserta??o (Mestrado) ? Programa Multic?ntrico de P?s-gradua??o em Ci?ncias Fisiol?gicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:34:44Z No. of bitstreams: 5 jus.pdf: 3394180 bytes, checksum: 71d0ff7761a41fcaa36b37cfba79f687 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T10:26:30Z (GMT) No. of bitstreams: 5 jus.pdf: 3394180 bytes, checksum: 71d0ff7761a41fcaa36b37cfba79f687 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-20T10:26:30Z (GMT). No. of bitstreams: 5 jus.pdf: 3394180 bytes, checksum: 71d0ff7761a41fcaa36b37cfba79f687 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-12-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Atualmente os nanotubos de carbono (NTC) representam um importante grupo de nanomateriais com atrativas propriedades qu?micas, eletr?nicas e mec?nicas. Estes materiais podem ser combinados a pol?meros, de maneira a moldar as propriedades f?sicas desses compostos (nanotubos-pol?mero), aumentando sua condutividade el?trica, estabilidade qu?mica e resist?ncia mec?nica. O presente trabalho apresenta o desenvolvimentoum material comp?sitonanoestruturado ? base de polianilina (PANI) e nanotubos de carbono de paredes m?ltiplas(MWCNT), para tal, os NTC foram funcionalizados com agrupamentos amina (PEG-NH2). A inser??o do grupamento amina junto aos NTC (PEG-MWCNT) foi confirmada atrav?s da caracteriza??o deste material utilizando as t?cnicas de Microscopia Eletr?nica de Varredura (MEV), Espectroscopia de infravermelho com transformada de fourrier(FT-IR) e Espectroscopia de Energia dispersiva de Raios-X. Foi produzido um eletrodo modificado atrav?s da polimeriza??o do mon?mero anilinaem presen?a dos NTC funcionalizados denominado PANI/PEG-MWCNT. Foram analisadas as propriedades do comp?sito PANI/PEG-MWCNT pelas t?cnicas de Voltametria c?clica e Espectroscopia de Imped?ncia Eletroqu?mica, atrav?s das quais foi verificado que a presen?a combinada dos NTC e da PANI favorece forma??o de um material comp?sito com propriedades sin?rgicas. Al?m disso, atrav?s da Microbalan?a Eletroqu?mica de Cristal de Quartzo pode-se constatarque a dopagem da PANI com PEG-MWCNT favorece o transporte de pr?tons significativamente. Atrav?s da t?cnica de microscopia eletroqu?mica de varredura foi poss?vel evidenciar que existem mais s?tios ativos na superf?cie do filme de PANI/PEG-MWCNT do que na superf?cie da PANI devido ? incorpora??o uniforme dos MWCNT. Finalmente, o eletrodo modificado com o material comp?sito foi utilizado como plataforma no desenvolvimento de um biossensor enzim?tico, pela imobiliza??o da enzima HRP (HRP/PANI/PEG-MWCNT). O biossensor HRP/PANI/PEG-MWCNT apresentou um ?timo suporte para aredu??o de per?xido de hidrog?nio a baixo potencial (-100 mV) vs Ag/AgCl, bem como alta sensibilidade (1.01) ?Acm-2 ?M?1e baixo limite de detec??o de 0,5 ?M L-1. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT Currently carbon nanotubes (NTC) represent an important group of nanomaterials with attractive properties (chemical, electronic and mechanical). These materials can be combined with polymers in order to shape their physical properties of these compounds (nanotube-polymer), increasing its electrical conductivity, chemical stability and mechanical strength. In fact, this study shows the production of a nanostructured composite material based on polyaniline (PANI) and carbon nanotubes multi-walled (MWCNT), for this, the NTC were functionalized with amine groups (PEG-NH2). The insertion of the amine grouping together to NTC (MWCNT-PEG) was confirmed by the characterization of this material using the techniques of Scanning Electron Microscopy (SEM) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and energy dispersive X-Ray. A modified electrode was produced through polymerization in the presence of PANI called NTC functionalized PANI/PEG-MWCNT. The properties of the composite PANI/MWCNT-PEG were examined by cyclic voltammetry and Electrochemical Impedance Spectroscopy, it was found that the combined presence of the NTC and polyaniline in the modified electrode provides a formation of a composite material with synergistic properties. Furthermore, through the technique of Electrochemical Microbalance Quartz Crystal can be seen that doping of PANI with PEG-MWCNT facilitates significantly the transport of protons. Finally, through the technique of scanning electrochemical microscopy was possible to show that there are more active sites on the surface of the film of PANI / MWCNT-PEG than on the surface of PANI due to the incorporation of MWCNT uniform. Lastly, the modified electrode composite material was used as a support for a biosensor for immobilization of the enzyme HRP (HRP/PANI/PEG-MWCNT). The biosensor HRP/PANI/MWCNT-PEG showed a great support for the reduction of hydrogen peroxide at a low potential (-100mv) vs Ag / AgCl as well as high sensitivity (1.01) ?A cm-2 mM-1 and low detection limit 0.5 ?m.

Submitted by Nivaldo Melo (nivaldo.melo@ufvjm.edu.br) on 2015-11-30T17:33:07Z No. of bitstreams: 2 poliana_ribeiro_barroso.pdf: 8014555 bytes, checksum: 744e34507fbe1725f369a03ee8c97123 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-01T16:10:43Z (GMT) No. of bitstreams: 2 poliana_ribeiro_barroso.pdf: 8014555 bytes, checksum: 744e34507fbe1725f369a03ee8c97123 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-12-01T16:10:43Z (GMT). No. of bitstreams: 2 poliana_ribeiro_barroso.pdf: 8014555 bytes, checksum: 744e34507fbe1725f369a03ee8c97123 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2015 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / As plantas medicinais s?o fontes promissoras de novas drogas, em que a regi?o do Cerrado se destaca pela sua vasta biodiversidade. Dentre as plantas medicinais utilizadas nesta regi?o se encontra a esp?cie Miconia ferruginata DC. que ? conhecida como pixirica-do-campo ou babaten?o. Esta esp?cie ? utilizada no tratamento de doen?as de pele, e outras de origens inflamat?rias, parasit?rias e infecciosas, por?m os estudos qu?micos s?o escassos. Assim, os objetivos deste trabalho foram avaliar o perfil qu?mico e o potencial biol?gico da M. ferruginata (Melastomataceae). A esp?cie foi coletada no munic?pio de Diamantina, MG do qual foram preparados os extratos aquosos e etan?licos das folhas/flores e do caule. Por meio da triagem fitoqu?mica e das an?lises de CLAE-DAD-EM obteve-se um perfil qu?mico rico em compostos fen?licos. Foi poss?vel a identifica??o da presen?a de taninos, ?cidos fen?licos, flavonoides, e derivados glicosilados da quercetina. Pela an?lise dos constituintes vol?teis por CG-EM foi poss?vel identificar a presen?a de quatorze compostos, sendo eles pertencentes as classes dos sesquiterpenos, hidrocarbonetos, monoterpenos, fenilpropanoides e alco?is. No qual os sesquiterpenos ?-cariofileno e ?-humuleno foram os compostos majorit?rios, os quais s?o associados na literatura a diversas atividades biol?gicas, em especial a a??es anti-inflamat?rias, antif?ngicas e antitumorais. A an?lise das fra??es por CG-EM e CLAE-DAD foi poss?vel identificar catequinas, ?cido g?lico e flavon?is nos extratos aquosos e ?steres graxos e as subclasses das flavonas e flavon?is nos extratos etan?licos. Nas avalia??es biol?gicas os extratos etan?licos e aquosos apresentaram baixa toxicidade em c?lulas de mam?feros, em que a concentra??o selecionada para a avalia??o das demais atividades biol?gicas foi de at? 500 ?g/mL. Para a atividade antitumoral, os extratos apresentaram um grande potencial concentra??o dependente, com valores de IC50 variando de 56,44 a 180,4 ?g/mL. Bem como o tempo de exposi??o aumentou a pot?ncia de todos os extratos. Tamb?m foi observado que os extratos aquosos promoveram a inibi??o da prolifera??o de linf?citos estimulados por mit?genos, apresentando uma efic?cia em rela??o ? dexametasona, de 39,94% para o extrato das folhas a 45 ?g/mL, e de 57,3%, 137,4% e 251,8% para os extratos do caule a 15, 20 e 30 ?g/mL, respectivamente. Este efeito proliferativo pode estar relacionado ao potencial anti-inflamat?rio da M. ferruginata, relatada na medicina popular. Possivelmente, as atividades biol?gicas est?o relacionadas ao rico perfil qu?mico desta esp?cie, principalmente em compostos fen?licos. Para as atividades antimicrobianas, tripanocida 9 e leishmanicida n?o foram observadas a inibi??o do crescimento ou toxicidade frentes as cepas avaliadas. Desta forma este trabalho contribuiu para o conhecimento qu?mico e biol?gico da M. ferruginata. Por?m ensaios futuros utilizando as fra??es e subst?ncias isoladas, se fazem necess?rios para fortalecer os dados aqui apresentados e para demonstrar quais os mecanismos moleculares envolvidos nas atividades biol?gicas investigadas. / Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT Medicinal plants are reassuring sources of new drugs, in which the Cerrado region stands out for its broad biodiversity. Among the medicinal plants used in this same region is the species Miconia ferruginata DC. which is also known as pixirica-docampo or babaten?o. This species is used in the treatment of skin diseases, and other inflammatory, infectious and parasitic sources, despite the chemical studies about it are lacking. Therefore, the objectives of this study were to evaluate the chemical profile and biological potential of the M. ferruginata (Melastomataceae).The species was collected in Diamantina, MG, in which it was prepared for water and ethanol extraction of the leaves/flowers and stem. Through phytochemical screening and analysis of HPLC-MSDAD, we obtained a rich chemical profile in phenolic compounds. The identification of tannins, phenolic acids, flavonoids, glycosides of quercetin and derivativeshas been detected. For the analysis of volatile constituents by GC-MS, it was possible to identify the presence of fourteen compounds, namely belonging classes of sesquiterpenes, hydrocarbons, monoterpenes, phenylpropanoids and alcohols. For that,? and?- caryophyllene, humulenesesquiterpenes were the main components, which are associated in the literature several biological activities, especially anti-inflammatory, antifungal and antitumor activities.In the analysis of the fractions by GC-MS and HPLC-DAD, we identified catechins, gallic acid and flavonols in aqueous extracts,including fatty esters and subclass of flavones and flavonols in ethanol extracts. Within the biological evaluations, aqueous and ethanolic extracts showed low toxicity to mammalian cells, wherein the concentration selected for analysis of other biological activities was up to 500 ?g/mL. For antitumor activity, the extracts showed a great potential concentration-dependent, with IC50 values ranging from 56.44 to 180.4 ?g/mL. Just as the exposure time increased the potency of all extracts, it was also observed that aqueous extracts promoted inhibition of the proliferation of mitogen-stimulated lymphocytes, showing a efficacy when compared to dexamethasone, 39.94% for the extract from the leaves of 45 ?g/mL, and 57.3%, 137.4% and 251.8% for the extracts of stem of 15.20 and 30 ?g/mL, respectively. This proliferative effect may be related to the anti-inflammatory potential of M. ferruginata, reported in folk medicine. Possibly, the biological activities are related to the rich chemical profile of this species, mainly in phenolic compounds. For the antimicrobial, trypanocidal and leishmanicidal activities, we have not observed growth inhibition or toxicity fronts of the evaluated strains. Thus 11 this work contributed to the chemical and biological knowledge of M. ferruginata. Nevertheless, future trials using isolated fractions and substances are needed to strengthen the data presented here and to demonstrate the molecular mechanisms involved in the biological activities investigated. It is likely that the biological activities are related to the rich chemical profile of this species, mainly regarding phenolic compounds. For antimicrobial activity, trypanocidal and leishmanicidal, it was not observed growth inhibition or toxicity regarding the evaluated strains. Thus, this work contributed to the chemical and biological knowledge of the M. ferruginata. However, future trials using isolated fractions and substances are needed to strengthen the data presented here and to demonstrate the molecular mechanisms involved in the biological activities hereby investigated.

Submitted by Raniere Barreto (raniere.barros@ufvjm.edu.br) on 2018-05-04T19:18:26Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) keilla_dayane_silva_oliveira.pdf: 2387961 bytes, checksum: 0dfa7c33888cb64e6956fe93ebe9009a (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-05-14T13:50:45Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) keilla_dayane_silva_oliveira.pdf: 2387961 bytes, checksum: 0dfa7c33888cb64e6956fe93ebe9009a (MD5) / Made available in DSpace on 2018-05-14T13:50:45Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) keilla_dayane_silva_oliveira.pdf: 2387961 bytes, checksum: 0dfa7c33888cb64e6956fe93ebe9009a (MD5) Previous issue date: 2017 / Esta disserta??o, parte da an?lise dos perfis de gest?o adotada pelas incubadoras tecnol?gicas de cooperativas populares (ITCPs) vinculadas ?s universidades federais no Estado de Minas Gerais, durante os anos de 2015 a 2016. Trata-se de um estudo explorat?rio descritivo, de abordagem qualitativa e quantitativa, seu objeto de estudo ? an?lise de multicasos. A proposta de quatro ITCPs resulta da localiza??o das incubadoras no Estado de Minas Gerais, assim como seu hist?rico de funcionamento, al?m das quais ferramentas e processos gerenciais, elementos comuns e divergentes do processo de gest?o das ITCPs, infraestrutura das incubadoras, organiza??o gerencial, identifica??o e an?lise dos principais pontos fortes e fracos, oportunidades e amea?as das Incubadoras Tecnol?gicas de Cooperativas Populares. A coleta dos dados foi realizada por meio de question?rio estruturado, pesquisa bibliogr?fica e documental. Com base na an?lise dos dados, pode-se afirmar que os tipos de gest?o analisadas foram a autogest?o e a gest?o social, ambos os modelos desenvolvem suas atividades e processos similares, as diferen?as encontra-se de acordo com a realidade vivenciada por cada incubadora e a universidade em que est? inserida. Nota-se tamb?m que a atividade mais importante e que influencia na gest?o ? o processo de incuba??o. / Disserta??o (Mestrado Profissional) ? Programa de P?s-Gradua??o em Tecnologia, Sa?de e Sociedade, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / This dissertation, part of the analysis of the management profiles adopted by technological incubators of popular cooperatives (ITCPs) linked to universities in the State of Minas Gerais, during the years 2015 to 2016. This is an exploratory, descriptive, qualitative and quantitative approach, its object of study is multisite analysis. The proposal of four ITCPs results from the location of the incubators in the State of Minas Gerais, as well as their operating history, in addition to which management tools and processes, common and divergent elements of ITCP management process, incubator infrastructure, management organization, identification and analysis of the main strengths and weaknesses, opportunities and threats of Technological Incubators of Popular Cooperatives. The data were collected through a structured questionnaire, bibliographical and documentary research. Based on the analysis of the data, it can be affirmed that the types of management analyzed were self-management and social management, both models develop their activities and similar processes, the differences are in accordance with the reality experienced by each incubator and the university in which it is inserted. It is also noted that the most important activity and that influences the management is the incubation process.

Made available in DSpace on 2014-12-17T14:10:17Z (GMT). No. of bitstreams: 1 ErmetonDN.pdf: 732618 bytes, checksum: 6b2f580e4b65ea3ec17c1996913b4a02 (MD5) Previous issue date: 2008-12-05 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The Human Papillomavirus (HPV) infection is the major sexually transmitted disease all over the world. There are many factors associated to infection and the virus persistency in the organism. This study aims to evaluate the women's knowledge, attitudes and practice about the Papanicolaou test (Pap), as well as analyze the HPV and Chlamydia trachomatis infections prevalences in sexually active women from the city of S?o Jos? do Mipibu/RN/Brazil. This research was divided in two steps (step I and step II), using different methodologies and samples each. The samples collected in each step, even socio-demographic or from uterus cervix, are from different patients e were analyzed separated. In step I was evaluated 267 rural and urban zone women s knowledge, attitudes and practices about the Pap by home interview. In the step II were included 605 women with age ranged from 15 to 71 years old, with mean of 33,5 years old and from each one were collected two cervical samples, one for Pap and other for molecular biology, beside the epidemiological interview to investigate the correlation between prevalence of HPV infection and risk factors. To molecular analyses, the samples were processed using a mammal rapid DNA extraction technique protocol. For C. trachomatis DNA detection were used the CP24/27 primers, and GP5+/GP6+ to HPV. PCR products were analyzed by electrophoresis on 8% polyacrylamide gels, followed by silver staining. The results of the step I showed that, in spite of only 46,1% of the interviewed women they have demonstrated to possess appropriate knowledge on the Pap test, the attitude and practice proportions were significantly larger, 63,3% and 64,4% respectively. The largest education degree presented association with adaptation of the knowledge, attitudes and practice, while neglect, lack of solicitation of the exam for the doctor and shame, came as main barriers for the accomplishment of the exam. In the stage II the HPV general prevalence was 28,9%, being 26,7% in the women with normal cytology or benign alterations, 26,7% in the ones that had atypical squamous cells of undetermined significance (ASC-US) and 80% in those with Low grade squamous intraepithelial lesion (LSIL). the HPV infection prevalence was larger in the patients with up to 30 years of age and in the unmarried women, and those that had more than one sexual partner presented larger infection risk. The results show that the sexual relationship with multiple partners increased the infection risk for HPV and consequently the possibility of the occurrence of lesions uterine cervix / O v?rus do papiloma humano (HPV) representa um dos principais agentes sexualmente transmiss?veis entre as popula??es humanas de todo o mundo. V?rios fatores est?o associados ? infec??o e a persist?ncia desse v?rus no organismo. A infec??o produtiva pelo HPV resulta em altera??es no epit?lio da c?rvice uterina que podem evoluir para les?es de diferentes graus inclusive as malignas. Tais les?es podem ser visualizadas atrav?s do exame citol?gico de Papanicolaou (Pap), que apesar de simples, tem alto valor como teste de triagem na preven??o do c?ncer de colo do ?tero. Este estudo constou de duas etapas, com metodologias e abordagens distintas. O material coletado em cada etapa, sejam dados s?cio-demogr?ficos ou esp?cimes da c?rvice uterina para an?lise, foram obtidas de pacientes diferentes e foram analisados separadamente. Na etapa I, avaliou-se o conhecimento, a atitude e a pr?tica das mulheres em rela??o ao Pap por meio de entrevistas domiciliares de 267 mulheres das zonas rural e urbana do munic?pio de S?o Jos? do Mipibu, utilizando question?rio estruturado. Na etapa II, foram inclu?das 605 mulheres com idade variando de 15 a 71 anos, media de 33,5 anos das quais foram coletados dois esp?cimes cervicais, sendo um para o exame citol?gico e o outro para an?lise molecular. Um question?rio epidemiol?gico foi utilizado para obter informa??es, visando identificar fatores de risco associados ? infec??o. Para a an?lise molecular as amostras foram processadas utilizando protocolo de extra??o r?pida de DNA de mam?feros. Para detec??o do DNA da C. trachomatis e do HPV foi utilizada a t?cnica de PCR com os iniciadores CP24/27, e GP5+/GP6+ respectivamente. Os produtos de PCR foram submetidos ? eletroforese em gel de poliacrilamida e corados pela prata. Do total de mulheres analisadas na etapa I apenas 46,1% demonstram possuir conhecimento adequado sobre o teste de Pap. As propor??es de atitude e pr?tica adequadas foram significativamente maiores, 63,3% e 64,4% respectivamente. As mulheres com maior grau de escolaridade apresentaram melhores ?ndices de adequa??o dos conhecimentos, atitudes e pr?tica. O descuido, a falta de solicita??o do exame pelo m?dico e a vergonha, se apresentaram como principais barreiras para a realiza??o do exame. Na etapa II encontrou-se uma preval?ncia geral do HPV de 28,9%, sendo 26,7% nas mulheres com citologia normal ou altera??es benignas, sendo 26,7% as que tinham atipia de c?lula escamosa de significado indeterminado (ASC-US) e 80% naquelas com les?o intraepitelial escamosa de baixo grau (LSIL). A preval?ncia da infec??o pelo HPV foi maior nas pacientes com at? 30 anos de idade, nas solteiras, e naquelas que tiveram mais de um parceiro sexual e estas apresentaram maior risco de aquisi??o da infec??o pelo HPV e para o desenvolvimento de les?es da c?rvice uterina

Made available in DSpace on 2014-12-17T14:13:52Z (GMT). No. of bitstreams: 1 CarlosRNB_DISSERT.pdf: 3053054 bytes, checksum: 21a986fcce16c387458463b332b62819 (MD5) Previous issue date: 2011-08-01 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Introdu??o: A aus?ncia de dados recentes sobre a infec??o pelo Trypanosoma cruzi na ?rea end?mica do estado do Rio Grande do Norte motivou a realiza??o de um inqu?rito soroepidemiol?gico amostral em moradores da zona rural da mesorregi?o oeste e do munic?pio de Caic?, na mesorregi?o central. M?todos: Dezesseis munic?pios foram sorteados, 15 da mesorregi?o oeste e um da central, com popula??o estimada em 83.852 indiv?duos. Foram coletadas 1.950 amostras de sangue na mesorregi?o oeste e 390 em Caic?. A pesquisa de anticorpos anti-T. cruzi foi realizada usando os kits Chagatest? ELISA, Chagatest? HAI-hemaglutina??o indireta, e a rea??o de imunofluoresc?ncia indireta. Nos soros com resultados indeterminados foi realizado o western blot TESAcruzi? para confirma??o da reatividade. Resultados: A estimativa da soropreval?ncia revelou 6,5% para a mesorregi?o oeste e 3,3% em Caic?. A soropositividade eleva-se progressivamente com a idade at? a quinta d?cada de vida em Caic? e na sexta d?cada no oeste. As preval?ncias mais elevadas foram encontradas em grupos de munic?pios localizados na ?rea central e geograficamente pr?ximos dessa mesorregi?o. Apenas o grau de escolaridade e o conhecimento do triatom?neo evidenciaram associa??o ? soropositividade. N?o foram identificados indiv?duos sororreativos com idade inferior a 18 anos e n?o houve evid?ncia de associa??o quanto ao sexo. Conclus?es: A infec??o pelo T. cruzi persiste mais elevada e concentrada em munic?pios da ?rea central da mesorregi?o oeste, mas sugerem o decl?nio da transmiss?o vetorial nessa mesorregi?o e em Caic?. As vari?veis epidemiol?gicas parecem n?o exercer influ?ncia na soropositividade, ? exce??o da escolaridade e conhecimento do triatom?neo nos sororreativos da mesorregi?o oeste