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1	MOLECULAR GENETIC ANALYSIS OF THE AXIN HOMOLOG PRY-1 IN REGULATING DEVELOPMENTAL AND POST-DEVELOPMENTAL PROCESSES IN CAENORHABDITIS ELEGANS Mallick, Avijit January 2022 (has links) My Ph.D. research project in the Bhagwati Gupta lab focuses on understanding the mechanism by which the Axin family of scaffolding proteins functions to regulate biological processes in multicellular eukaryotes. Towards this, I am using the nematode (worm) Caenorhabditis elegans as an animal model to investigate the role of one of the Axin homologs, PRY-1. Studies in various model systems and humans have shown that the Axin family of proteins plays crucial roles during cell proliferation, cell differentiation, and organ formation. Such a role of Axin depends on the negative regulation of the WNT signaling cascade. Consistent with these, alterations in Axin function are associated with developmental abnormalities and age-associated diseases such as axis duplication, neuroectodermal defect, and muscle degeneration. As a scaffolding protein, Axin family members bind to and recruit multiple protein partners that are both WNT dependent and independent. However, how Axin interacts with these factors to regulate molecular events is not well understood. While some Axin-interacting factors have been identified, many more remain to be discovered. My project deals with the identification and functional characterization of pry-1/Axin interactors in C. elegans. The key findings of my Ph.D. research are published in five peer-reviewed papers. Collectively, the results demonstrate that PRY-1 is necessary to regulate lipid metabolism, stress response, muscle health, and aging. I have shown that PRY-1 utilizes multiple pathways to control these diverse processes. Specifically, PRY-1 functions via the SREBP transcription factor homolog SBP-1 to regulate yolk lipoprotein expression to promote lipid synthesis. The analysis of pry-1’s role in aging and muscle health has revealed its interactions with the energy sensor AMPK homolog AAK-2, thereby affecting the function of the Insulin/IGF1 signaling (IIS) transcriptional regulator DAF-16/FOXO. Moreover, I have identified several mRNA genes and microRNAs that function downstream of PRY-1/Axin signaling to either suppress or enhance pry-1 mutant defects. All these novel interactors have mammalian homologs. Altogether, these findings form the basis to pursue future work to investigate the conserved mechanism of Axin signaling and hold the potential for effective intervention to delay aging and age-associated muscle deterioration. / Thesis / Doctor of Philosophy (PhD)
2	Genetic Analysis of Pry-1/Axin Function in the Nematode Caenorhabditis Briggsae / Genetic Analysis of PRY-1/Axin in Caenorhabditis Briggsae Bojanala, Nagagireesh 12 1900 (has links) Evolutionary variations during vulval development in C. elegans and its related nematode species are well analyzed. The formation of C. elegans vulva involves many complex cell-cell interactions that are mediated through well conserved EGF/EGFR/RAS, LIN12/Notch and WNT signaling pathways. These pathways specify distinct cell fates of the six epidermal vulval precursor cells (VPCs), P(3-8).p. pry-1/Axin in C. elegans is identified as a part of destruction box complex that mediates (beta)-catenin degradation and is known to negatively regulate canonical Wnt signaling pathway during its development. I focused on the genetic analysis of pry-1 I Axin function in C. briggsae, sister species to C. elegans, to study inter-species comparisons of vulva formation. Three alleles, lin(sy5353), lin(sy5411) and lin(sy5270) were genetically mapped to LG I using standard genetic and in del mapping techniques. Interestingly, a unique simultaneous Multi vulva and Vulvaless (Muv-Vul) phenotype was observed during vulva formation in Cbr-pry-1 alleles, resulting from the varying induction potentials of the VPCs along the A/P axis, compared to Cel-pry-1 animals. In order to analyze these phenotypic differences between Cel-pry-1 and Cbr-pry-1 in greater detail, I dissected vulval development in sy5353 animals. VPC competence analysis was done through cell lineages and ablations studies, while the C. briggsae vulval cell fate markers were used for cell fate specification analysis. Cell ablations revealed that P7.p and P8.p in Cbr-pry-1 animals exhibited non-competence towards anchor cell signaling. Additionally, gonad-independent inductions was observed in P(3-8).p cells and they adopted 2° cell fate specifications. Using RNAi approach, Cbr-pry-1 interactions with other vulval pathway genes were dissected and it was observed that Cbr-lin-12 is involved in VPC competence of P7.p and Cbr-pop-1 exhibited different regulatory levels during vulval development compared to C. elegans. Thus, it can be inferred that the mechanisms of vulva formation in C. briggsae has evolved through changes in the competence of VPCs. / Thesis / Master of Science (MS) genetic genetic analysis pry-1/axin nematode caenorhabditis caenorhabditis briggsae
3	Dr. Eleine Mad Jacobsson, Madeleine January 2021 (has links) Dr. Eleine Mad är Madeleine Jacobsssons talesperson för dom vetenskapliga och paranormala upptäckter som uppstår i hennes världar. Hon beskriver innehåll, teknik och estetik utifrån ett kategoriseringssytem där konsten delas upp som olika typer av komponenter och därefter avkodas dessa allteftersom. För att förstå intuitionens inblandning i arbetsprocessen omförvandlas den till tre separata roller av en Sökare, Samlare och Myntare. Med rollerna försöker jag beskriva på vilka sätt som intuitionen är till gagn eller av förödelse för det konstnärliga arbetet. Sagan om M handlar om en grodlik karaktär, Delop, som lämnar sin hemplanet för att uppsöka andra världar. I sitt sökande hittar Delop ett folkslag vars syn och levnadssätt skiljer sig från hennes erfarenheter av “verkligheten” såsom hon lärt sig att överleva i den. / Dr. Eleine Mad is Madeleine Jacobsson's spokesperson for the scientific and paranormal discoveries that arise in her worlds. She describes content, tecniques and aesthetics based on a categorization system where art is divided into different types of components and then decoded as they go. To understand the intuition's involvement in the work process, it is transformed into three separate roles by a Seeker, Collector and a Myntare(In swedish language the one who is a "myntare" -is verbally declaring a concept or term). With these roles I try to describe in what ways intuition is beneficial or devastating to the artistic work. The story of M is about a frog-like character, Delop, who leaves the home planet to seek out other worlds. In her search, Delop finds a world whose views and lifestyles differ from her experiences of "reality" as she learned to survive in it. / <p>Recorded sound and image material of the presentation is available for private use.</p> mjjgkjacb longing desire pang aj worlds shark genital symbol female sexuality worlds content the tale of M Doctor M mad life artifact animals figures emotional states red existentialism epicenter dog mythology folk beer whistle closeness aversions seeker collector myntare stagnation hibernation intuition sausage soup majdalakdemlsmikopa Delop Cow Lice snake hare sheep ödel oktor threat antipathy empathy fear performance function allow belief clitoral heroes monsters pink components situation dual nature euphoria bic-pens reality violence desert sea AQB brain holistics social codes self fairy tale space blue horse hell gotland dizzying flourish anthropomorphic jewels death plaster spiders commit survive frog whale bear purpose communication wild fascination animal incomparable monster ugly disturbed perspective marauder green symbolism who suffer anger reverence gods recast transformation yellow pry entrance interest alternative insignificant rigid position of power as disease real added disgusting wake up doubt mourn pray images horror plants rock stop mjjgkjacb längtan åtrå pang aj världar haj världsallt könssymbol kvinnlig sexualitet världarnas innehåll sagan om M Doktor M mad livet artefakt djur figurer känslomässiga tillstånd röd existentialism epicentrum hund mytologi folköl vissla närhet aversioner sökare samlare myntare stagnation dvala intuition korvsoppa majdalakdemlsmikopa Delop Ko Löss orm hare får Ödel Oktor hot antipati empati rädsla föreställning funktion tillåta tro klitorala hjältar monster rosa världar komponenter situation dubbla natur eufori bic-pennor verkligheten våld öken hav A.Q.B hjärna holistisk sociala koder självet sagorymd blå häst helvete Gotland vimmelkantig florera antropomorf juveler döden plåsterspindlar vägvisare begå överleva groda val björn syfte kommunicering vild fascination djurisk makalös vidunder ful störd perspektiv marodör grön symbolism vem genomlida ilska vördnad gudar omstöpt sammandrabbningar transformation gul bända ingång intresserar alternativ oväsentlig stel maktposition som sjukdom verkligt tillsatt äcklig vakna tvivla sörja be urbilder skräck växter rock stopp Arts Konst

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