Global ETD Search

61	Koevoluce tropických ptačích ektoparazitů / Coevolution of avian ectoparasites in the tropics Gajdošová, Magdalena January 2018 (has links) Host-parasite associations are born by cospeciations or by host switches. Feather lice have traditionally been used as model parasites for studying these events and underlying ecological factors. By now tens of analyses have addressed comparisons of host and parasite phylogenies to study cospeciations and host switches in lice, however, these analyses are strongly biased towards the temperate zone. Tropical environment could provide new insight into the origin of host-parasite interactions, because it is ecologically unique. This work aims to supplement the knowledge of host-parasite associations in lice using coevolution analyses of two feather lice genera and their passerine hosts in tropical rainforest in Cameroon. It shows that lice in the tropics cospeciate rarely. To assess whether host switches are non-random and occur preferentially between hosts with specific traits, this work also analyses relations between parasite genetic distances and hosts' trait similarities. No effect of host morphology and spatial distribution was found. However, genetic distances of the lice strongly correlate with genetic distances of their hosts.
62	Sustentabilidade das empresas associadas ? REDEPETRO-RN Rezende, Julio Francisco Dantas de 28 August 2012 (has links) Made available in DSpace on 2014-12-17T13:51:34Z (GMT). No. of bitstreams: 1 JulioFDR_TESE_capa_ate_pag119.pdf: 6154675 bytes, checksum: 174bd5531b27bc99f78b05dff75d611d (MD5) Previous issue date: 2012-08-28 / The present study investigated the management of social, environmental and economic sustainability practices employed by 72 oil companies in Rio Grande do Norte state in Brazil associated to Joint Business Network for Enhancing the Competitive Ability of Suppliers of Goods and Services to the Gas and Oil Industry in Rio Grande do Norte (REDEPETRO-RN). Thus, our investigation aimed to determine the extent to which sustainability practices of companies associated to REDEPETRO-RN are developed through their own efforts or influenced by the Triple Helix interorganizational arrangement (governamental organizations, education and research institutions, and industries). The research instrument used was a questionnaire in order to map and characterize the sustainability practices. The research subjects were business owners and managers. The data collected supported the descriptive, correspondence and correlation analyzes. Were identified a greater emphasis of the companies surveyed in developing sustainability practices and greater economic influence of business organizations in the construction of sustainability status. It was concluded with confirmation of thesis of the REDEPETRO-RN arrangement exhibits the characteristics and functioning of a common Interorganizational Cooperative Arrangement, not those of a triple helix interorganizational arrangement, given that the influences exerted for the development of social, environmental and economical sustainability on the part of teaching and research institutions, and government and business organizations do not interact with one another sufficiently to create a virtuous circle of cooperation among the associated companies. By contrast, the Interorganizational Cooperative Arrangement plays an important role in strengthening the competitiveness of companies affiliated to REDEPETRO-RN, by combining different competencies in an attempt at supporting the adoption of sustainability practices, a role reinforced by the scope of PETROBRAS, which, due to its economic importance, has considerable weight in the managerial decisions of associated companies / Esta pesquisa explorou a gest?o de pr?ticas de sustentabilidade social, ambiental e econ?mica de 72 empresas associadas ? Rede de Articula??o Empresarial para Amplia??o da Capacidade Competitiva dos Fornecedores de Bens e Servi?os do Setor de Petr?leo e G?s do Rio Grande do Norte (REDEPETRO-RN), procurando responder em que medida as pr?ticas de sustentabilidade das empresas associadas ? rede s?o explicadas por um esfor?o pr?prio ou s?o influenciadas pelo arranjo interorganizacional do tipo H?lice Tripla configurado pela REDEPETRO-RN. (organiza??es governamentais, institui??es de ensino e pesquisa e organiza??es empresariais). O instrumento utilizado foi o question?rio junto aos empres?rios e gestores. Os dados coletados subsidiaram a realiza??o de an?lises descritivas, de correspond?ncia e correla??o. Identificou-se uma maior ?nfase das empresas no desenvolvimento de pr?ticas de sustentabilidade econ?mica e uma maior influ?ncia das organiza??es empresariais na constru??o desse status de sustentabilidade, confirmando a tese de que o arranjo REDEPETRO-RN apresenta caracter?sticas e funcionamento de um Arranjo Cooperativo Interoganizacional (ACI) comum, n?o apresentando caracter?sticas de um arranjo interorganizacional do tipo H?lice Tripla, dado as influ?ncias exercidas para o desenvolvimento de pr?ticas de sustentabilidade social, ambiental e econ?mica por parte das institui??es de ensino e pesquisa, das organiza??es governamentais e empresariais n?o interagirem entre si suficientemente para configurar um c?rculo virtuoso de coopera??o entre as empresas associadas. No entanto, o Arranjo Cooperativo Interorganizacional tem um papel importante no fortalecimento da competitividade das empresas associadas ? REDEPETRORN, ao reunir diferentes compet?ncias e tentar articul?-las para apoiar entre outras a??es a ado??o de pr?ticas de sustentabilidade, papel valorizado pela for?a da PETROBRAS, que em fun??o de sua import?ncia econ?mica tem um peso consider?vel nas decis?es gerenciais das empresas associadas
63	A influ?ncia do modelo de triplice h?lice no grau de maturidade de inova??o: um estudo de caso em empresas participantes do projeto PR?-INOVA/NAGI no estado do RIO GRANDE DO NORTE Nunes, Benilton Medeiros 20 November 2013 (has links) Made available in DSpace on 2014-12-17T14:53:20Z (GMT). No. of bitstreams: 1 BeniltonMN_DISSERT.pdf: 2048130 bytes, checksum: e16a5a6536a18c42327ce9f1122bb943 (MD5) Previous issue date: 2013-11-20 / Public investments in the development of innovation in the country, either through the rigging of public universities, either through public announcements of the promotion, increased dramatically in recent years. To analyze the efficiency and effectiveness of the use of public resources is especially in times of austerity, essential for the development of a country. In this context, this research aims to identify the influence of public investments to promote innovation in the degree of maturity of innovative companies in the state of the RN. Another goal is to identify the regional influence from the installation site - capital or countryside, in the performance of the companies studied in the degree of innovation. The theoretical basis of the understanding of the scope of the concept of innovation and its determination for the purposes of this study. Typology, degree of innovation, evaluation methodologies and mechanisms to support innovation : Still on the theme of innovation additional concepts that help the reader to a greater understanding, such as are presented. Following is approached conceptualization of the triple helix, highlighting the concepts advocated by Etzkowitz and Leydesdorff, Salomon and Silva and Ipiranga, Freitas and Paiva, among others. With regard to methodological aspects, we propose a descriptive, exploratory and explanatory research quantitative and qualitative approach with the target audience group of companies served by INOVA PRO- NAGI design - multi-institutional action from a public resource called FINEP promotes the development of innovative companies in the State of Rio Grande do Norte - in 2013. The research should provide reflection and understanding of the influence of public investment in innovation, which by means of qualitative predictive variables associated with quantitative method to explain which variables are significant variations in the degree of maturity of enterprises studied / Os investimentos p?blicos no desenvolvimento da inova??o no pa?s, quer por meio do aparelhamento das universidades publicas, quer por meio de editais de fomento, cresceram vertiginosamente nos ?ltimos anos. Analisar a efici?ncia e efic?cia da aplica??o dos recursos p?blicos ?, sobretudo em tempos de austeridade, primordial para o desenvolvimento de um pa?s. Neste contexto, a presente pesquisa tem como objetivo identificar a influ?ncia dos investimentos p?blicos de fomento ? inova??o no grau de maturidade de inova??o de empresas instaladas no Estado do RN. Objetiva-se ainda identificar a influ?ncia regional, a partir do local de instala??o capital ou interior, no desempenho das empresas estudadas no grau de inova??o. A fundamenta??o te?rica parte da compreens?o da dimens?o do conceito sobre a inova??o e a sua defini??o para efeitos deste trabalho. Ainda sobre a tem?tica da inova??o s?o apresentadas conceitua??es adicionais que auxiliam o leitor a uma maior compreens?o, tais como: tipologia, graus de inova??o, metodologias de avalia??o e mecanismos de apoio ? inova??o. Na sequ?ncia ? abordada a conceitua??o sobre a tr?plice h?lice, ressaltando os conceitos preconizados por Etzkowitz e Leydesdorff, Salomon e Silva e Ipiranga, Freitas e Paiva, entre outros. No tocante aos aspectos metodol?gicos, prop?e-se uma pesquisa descritiva, explorat?ria e explicativa de abordagem quanti-qualitativa, tendo como p?blico-alvo o grupo de empresas atendidas pelo projeto PR?-INOVA NAGI a??o multiinstitucional que a partir de recursos de uma chamada p?blica da FINEP promove o desenvolvimento da inova??o em empresas do Estado do Rio Grande do Norte no ano de 2013. A pesquisa dever? proporcionar reflex?es e compreens?o da influ?ncia dos investimentos p?blicos em inova??o, que por meio de vari?veis qualitativas preditivas, associada ao m?todo quantitativo explicar quais vari?veis s?o significativas na varia??o do grau de maturidade em inova??o das empresas estudadas
64	The potential role of ABC transporters as factors influencing drug susceptibility in the salmon louse, Lepeophtheirus salmonis (Kroyer, 1837) Heumann, Jan H. January 2014 (has links) Efficient control of sea lice is a major challenge for the sustainable production of farmed Atlantic salmon (Salmo salar (Linnaeus, 1758)). These marine ectoparasites feed on mucus, skin and blood of their hosts, thereby reducing the salmon’s growth rate and overall health. In the northern hemisphere, the most prevalent species is Lepeophtheirus salmonis (Krøyer, 1837). In 2006, global costs of sea lice infections are estimated to have exceeded €300 million, with the majority spent on a limited number of chemical delousing agents. Emamectin benzoate (EMB; SLICE®), an avermectin, has been widely used since its introduction in 2000, due to its convenient administration as an in-feed medication and its high efficacy against all parasitic stages of L. salmonis. However, over-reliance on a single or limited range of medicines favours the emergence of drug resistance and, as a result, the efficacy of this compound in treating L. salmonis has decreased in recent years, as reported from e.g. Chile, Norway, Scotland and Canada. Declining efficacy underlines the need for an improved understanding of the molecular mechanisms underlying EMB drug resistance in L. salmonis. Elucidation of these mechanisms would allow for improved monitoring tools, earlier detection of developing resistance, extended usability of current delousing agents and development of new parasiticides. The work described in this thesis sets out to examine the molecular mechanisms underlying EMB resistance in L. salmonis. In earlier studies, research in nematodes and arthropods has linked drug efflux transporters belonging to the family of ATP-binding cassette (ABC) transporters to ivermectin (IVM) resistance, a parasiticide with high chemical similarity to EMB. ABC transporters such as permeability glycoprotein (P-gp), transport a wide range of substrates, including drugs, and have been suggested to provide a potential molecular mechanism through which EMB resistance might be mediated in sea lice. As an example of such mechanisms, increased expression of P-gp is one of the causative factors for drug resistance in human cancer cells and avermectin resistance in nematode parasites such as Caenorhabditis elegans or Haemonchus contortus. Initial research involved screening for novel salmon lice P-gps that might contribute to EMB resistance. A novel P-gp, SL-PGY1, was discovered using a combined bioinformatic and molecular biological approach. The expression was compared in two well-characterised L. salmonis strains differing in their susceptibility to EMB (S = susceptible, R = resistant). Prior to EMB exposure, mRNA levels did not differ from each other, while, after 24 h exposure, a 2.9-fold increase in SL-PGY1 mRNA expression was observed in the R strain. SL-PGY1 appears not to be a major factor contributing to reduced EMB susceptibility, although it could play a role, as expression levels increased upon exposure to EMB. A further four additional drug transporters (ABC C subfamily) were also discovered showing high homology to multidrug-resistance proteins (MRP). The relative expression levels of each MRP was compared in the strains S and R, before and after exposure to EMB. No significant changes were found in their expression patterns. If ABC drug transporters mediate the efflux of EMB and thereby reduce the intracellular concentrations of the drug in exposed animals, the inhibition of those ABC drug transporters was expected to lead to higher intracellular levels of EMB. This could result in an enhanced toxic effect when EMB is co-administered with an inhibitor. Two known inhibitors of human P-gps and MRPs, cyclosporin A (CSA) and verapamil (VER), were co-administered with EMB. CSA increased the toxic effect of EMB in both tested strains, implying that the targets of CSA are expressed at comparable levels and that they may be part of the mechanism conferring EMB resistance. VER increased the toxic effect of EMB in the R strain, but had no significant effects on the S strain. This implies that the expression of factors inhibited by VER differs between the two L. salmonis strains. It is hypothesised that a number of ABC transporters with distinct, yet overlapping patterns of inhibitor specificity are affected by those inhibitors. The search for drug-resistance conferring genes was complemented with a systematic, genome-wide survey of ABC transporters in L. salmonis to find additional members of this important gene family. Next-generation high-throughput RNA sequencing (RNA-seq) was employed to assemble a reference transcriptome from pooled total RNA of salmon lice at different development stages. The transcriptome was assembled against the L. salmonis genome and annotated. Thirty-nine putative ABC transporters were found. Of further interest were transcripts of the subfamily B, C and G, as they contain drug-transporting ABC proteins. For the ABC B subfamily, one full (SL-PGY1) and three half transporter transcripts were found. Only full transporters are known to transport drugs and SL-PGY1 is apparently not a major factor contributing to EMB resistance. Fourteen ABCC sequences were found – 11 MRPs and 3 homologues to sulfonylurea receptors. Of interest are MRPs, as they contribute to drug detoxification in humans and invertebrates. Four MRPs had been identified previously and their expression ratios did not differ between S and R strain parasites. Seven sequences belonging to ABCG subfamily were found. However, none of the L. salmonis ABCG transcripts identified showed sufficient homology to known drug transporters in other species. With the currently limited understanding of the mechanisms conferring EMB resistance, monitoring the susceptibility of L. salmonis subpopulations is essential. Dose-response bioassays are currently widely used. Tests with pre-adult II or adult parasites requires relatively large numbers of parasites (~150) to conduct this type of bioassay, which may not always be available. Addressing this issue, we tested the feasibility of a single-dose bioassay (requiring fewer test animals than dose-response bioassays) to discriminate between L. salmonis strains with differing EMB susceptibility. This alternative approach uses time-course toxicity analysis, where the toxic effect of EMB is monitored over time. After clearly defining the effect criteria, we found that it is possible to discriminate between those L. salmonis strains. However, while requiring fewer test animals, time course toxicity analysis is more labour-intensive, but the alternative design can be suitable under certain circumstances. The work reported here has provided new knowledge concerning the mechanisms of EMB resistance in sea lice. Several novel putative drug transporters have been identified, an important first step toward unravelling the complex interactions of genes involved in EMB resistance in this commercially important parasite. 639.3
65	Comparison of the Humoral Immune Response following Both Bacterial Challenge and RNAi of Major Factors on Proliferation of Bartonella quintana in the Human Louse Zina, Jake 28 October 2022 (has links) (PDF) Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their feces. In this study, we first investigated this differential immune response by performing RNAi to knockdown defensin 1 by dsRNA injection. B. quintana was orally infected 72 h after injection and proliferation was compared at 2 hours (day 0) and day 4 post-infection. At day 0, bacterial cell numbers increased 1.5-fold in defensin 1 (Def1(-)) knocked down head lice compared with non-knocked down, pQE30-dsRNA injected, head lice control. At day 4, Def1(-) knocked down head lice had 2.55-fold more bacterial cells than control head lice and 1.65-fold greater than body lice, indicating that defensin 1 was active in reducing B. quintana cell number in non-knocked down head lice. Second, the levels of cytotoxic reactive oxygen species (ROS) generated by the epithelial cells of the alimentary tract were measured using two general indictors of ROS in both body and head lice at day 1 and day 4 following B. quintana challenge. Challenged body lice showed a 42% and 34% increase in ROS, whereas head lice showed a 70% and 22% increase at day 1 using CM-H2DCFDA and HPF as general indicators, respectively. On day 4, all challenged lice showed similar ROS levels except for body lice which maintained their ROS levels (40% increase using CM-H2DCFDA). Head lice are likely to have multiple immune and/or non-immune factors that suppress B. quintana proliferation, and the production of sustained ROS levels and/or the single knockdown of Defensin 1 is not enough to increase B. quintana proliferation in head lice to that seen in body lice. lice louse Pediculus humanus RNAi Bartonella quintana immune response Animal Sciences Bacteria Bacterial Infections and Mycoses Bacteriology Biology Biotechnology Entomology Immunity Immunology of Infectious Disease Other Animal Sciences Other Immunology and Infectious Disease Other Veterinary Medicine Pathogenic Microbiology Toxicology Veterinary Infectious Diseases
66	Dr. Eleine Mad Jacobsson, Madeleine January 2021 (has links) Dr. Eleine Mad är Madeleine Jacobsssons talesperson för dom vetenskapliga och paranormala upptäckter som uppstår i hennes världar. Hon beskriver innehåll, teknik och estetik utifrån ett kategoriseringssytem där konsten delas upp som olika typer av komponenter och därefter avkodas dessa allteftersom. För att förstå intuitionens inblandning i arbetsprocessen omförvandlas den till tre separata roller av en Sökare, Samlare och Myntare. Med rollerna försöker jag beskriva på vilka sätt som intuitionen är till gagn eller av förödelse för det konstnärliga arbetet. Sagan om M handlar om en grodlik karaktär, Delop, som lämnar sin hemplanet för att uppsöka andra världar. I sitt sökande hittar Delop ett folkslag vars syn och levnadssätt skiljer sig från hennes erfarenheter av “verkligheten” såsom hon lärt sig att överleva i den. / Dr. Eleine Mad is Madeleine Jacobsson's spokesperson for the scientific and paranormal discoveries that arise in her worlds. She describes content, tecniques and aesthetics based on a categorization system where art is divided into different types of components and then decoded as they go. To understand the intuition's involvement in the work process, it is transformed into three separate roles by a Seeker, Collector and a Myntare(In swedish language the one who is a "myntare" -is verbally declaring a concept or term). With these roles I try to describe in what ways intuition is beneficial or devastating to the artistic work. The story of M is about a frog-like character, Delop, who leaves the home planet to seek out other worlds. In her search, Delop finds a world whose views and lifestyles differ from her experiences of "reality" as she learned to survive in it. / <p>Recorded sound and image material of the presentation is available for private use.</p> mjjgkjacb longing desire pang aj worlds shark genital symbol female sexuality worlds content the tale of M Doctor M mad life artifact animals figures emotional states red existentialism epicenter dog mythology folk beer whistle closeness aversions seeker collector myntare stagnation hibernation intuition sausage soup majdalakdemlsmikopa Delop Cow Lice snake hare sheep ödel oktor threat antipathy empathy fear performance function allow belief clitoral heroes monsters pink components situation dual nature euphoria bic-pens reality violence desert sea AQB brain holistics social codes self fairy tale space blue horse hell gotland dizzying flourish anthropomorphic jewels death plaster spiders commit survive frog whale bear purpose communication wild fascination animal incomparable monster ugly disturbed perspective marauder green symbolism who suffer anger reverence gods recast transformation yellow pry entrance interest alternative insignificant rigid position of power as disease real added disgusting wake up doubt mourn pray images horror plants rock stop mjjgkjacb längtan åtrå pang aj världar haj världsallt könssymbol kvinnlig sexualitet världarnas innehåll sagan om M Doktor M mad livet artefakt djur figurer känslomässiga tillstånd röd existentialism epicentrum hund mytologi folköl vissla närhet aversioner sökare samlare myntare stagnation dvala intuition korvsoppa majdalakdemlsmikopa Delop Ko Löss orm hare får Ödel Oktor hot antipati empati rädsla föreställning funktion tillåta tro klitorala hjältar monster rosa världar komponenter situation dubbla natur eufori bic-pennor verkligheten våld öken hav A.Q.B hjärna holistisk sociala koder självet sagorymd blå häst helvete Gotland vimmelkantig florera antropomorf juveler döden plåsterspindlar vägvisare begå överleva groda val björn syfte kommunicering vild fascination djurisk makalös vidunder ful störd perspektiv marodör grön symbolism vem genomlida ilska vördnad gudar omstöpt sammandrabbningar transformation gul bända ingång intresserar alternativ oväsentlig stel maktposition som sjukdom verkligt tillsatt äcklig vakna tvivla sörja be urbilder skräck växter rock stopp Arts Konst

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